CAST / Calpastatin Antibody (clone CSL1-5) LS-C83579
LifeSpan Biosciences
catalog: LS-C83579
mouse monoclonal (CSL1-5)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - frozen section
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CAST / Calpastatin Antibody (clone 1F7E3D10) LS-C84821
LifeSpan Biosciences
catalog: LS-C84821
mouse monoclonal (1F7E3D10)
reactivity: human, bovine
application: western blot, immunocytochemistry
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CAST / Calpastatin Antibody (clone 2G11D6) LS-C84822
LifeSpan Biosciences
catalog: LS-C84822
mouse monoclonal (2G11D6)
reactivity: human, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
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CAST / Calpastatin Antibody LS-C96171
LifeSpan Biosciences
catalog: LS-C96171
chicken polyclonal
reactivity: human
application: western blot, ELISA
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CAST / Calpastatin Antibody LS-C155216
LifeSpan Biosciences
catalog: LS-C155216
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE CAST / Calpastatin antibody diluted at 1:2000

IHC of paraffin-embedded Breast ca, using Calpastatin antibody at 1:250 dilution.
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CAST / Calpastatin Antibody (clone 2C5) LS-C196815
LifeSpan Biosciences
catalog: LS-C196815
mouse monoclonal (2C5)
reactivity: human
application: western blot, ELISA, immunocytochemistry

CAST monoclonal antibody (M03), clone 2C5. Western Blot analysis of CAST expression in NIH/3T3.

Immunofluorescence of monoclonal antibody to CAST on HeLa cell . [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged CAST is 0.03 ng/ml as a capture antibody.
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CAST / Calpastatin Antibody LS-C306745
LifeSpan Biosciences
catalog: LS-C306745
mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry
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CAST / Calpastatin Antibody LS-C309725
LifeSpan Biosciences
catalog: LS-C309725
domestic rabbit polyclonal
reactivity: human
application: western blot
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CAST / Calpastatin Antibody (Biotin) LS-C317747
LifeSpan Biosciences
catalog: LS-C317747
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human prostate using CAST / Calpastatin antibody at dilution of 1:10. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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CAST / Calpastatin Antibody (HRP) LS-C317751
LifeSpan Biosciences
catalog: LS-C317751
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human prostate using CAST / Calpastatin antibody at dilution of 1:10. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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CAST / Calpastatin Antibody (FITC) LS-C317754
LifeSpan Biosciences
catalog: LS-C317754
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human prostate using CAST / Calpastatin antibody at dilution of 1:10. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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CAST / Calpastatin Antibody LS-C330790
LifeSpan Biosciences
catalog: LS-C330790
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of H460 cells, using CAST antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunohistochemistry of paraffin-embedded rat heart using CAST antibodyat dilution of 1:100 (40x lens).
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CAST / Calpastatin Antibody (clone OTI1E7) LS-C337646
LifeSpan Biosciences
catalog: LS-C337646
mouse monoclonal (OTI1E7)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAST (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAST.

IHC of paraffin-embedded Human colon tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
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