ACTN4 Antibody (aa28-38) LS-C20281
LifeSpan Biosciences
catalog: LS-C20281

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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IHC-plus™ ACTN4 Antibody (clone 4D10) LS-B5374
LifeSpan Biosciences
catalog: LS-B5374

mouse monoclonal (4D10)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody (M01), clone 4D10.

Immunofluorescence of monoclonal antibody to ACTN4 on HeLa cell. [antibody concentration 20 ug/ml]

Detection limit for recombinant GST tagged ACTN4 is approximately 0.1 ng/ml as a capture antibody.
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ACTN4 Antibody (aa201-250) LS-C40369
LifeSpan Biosciences
catalog: LS-C40369

domestic rabbit polyclonal
reactivity: human
application: western blot

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ACTN4 Antibody (aa800-849) LS-C40370
LifeSpan Biosciences
catalog: LS-C40370

domestic rabbit polyclonal
reactivity: human
application: western blot

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ACTN4 Antibody LS-C96165
LifeSpan Biosciences
catalog: LS-C96165

domestic rabbit polyclonal
reactivity: human
application: ELISA

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ACTN4 Antibody (aa192-220) LS-C96726
LifeSpan Biosciences
catalog: LS-C96726

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of anti-ACTN4 Antibody in HeLa cell line lysates (35 ug/lane). ACTN4 (arrow) was detected using the purified antibody.
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ACTN4 Antibody (aa785-813) LS-C96727
LifeSpan Biosciences
catalog: LS-C96727

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of ACTN4 Antibody in T47D, A2058, A375, HepG2 cell line and mouse bladder tissue lysates (35 ug/lane). ACTN4 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ACTN4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric of MDA-231 cells using ACTN4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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ACTN1+2+3+4 Antibody (aa21-70) LS-C119374
LifeSpan Biosciences
catalog: LS-C119374

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from 293, HeLa, and A549 cells, using ACTN1/2/3/4 Antibody. The lane on the right is blocked with the synthesized peptide.
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ACTN4 Antibody (N-Terminus) LS-C144671
LifeSpan Biosciences
catalog: LS-C144671

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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ACTN4 Antibody (N-Terminus) LS-C153368
LifeSpan Biosciences
catalog: LS-C153368

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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ACTN4 Antibody (aa397-648) LS-C178740
LifeSpan Biosciences
catalog: LS-C178740

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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ACTN4 Antibody (aa206-542) LS-C186290
LifeSpan Biosciences
catalog: LS-C186290

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: A431 , B: Hep G2 . 7.5% SDS PAGE. ACTN4 antibody diluted at 1:1000.

Immunofluorescence of paraformaldehyde-fixed HeLa using alpha Actinin 4 antibody at 1:200 dilution.

IHC of paraffin-embedded A549 xenograft using alpha Actinin 4 antibody at 1:500 dilution.
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ACTN4 Antibody LS-C309314
LifeSpan Biosciences
catalog: LS-C309314

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

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ACTN4 Antibody LS-C332459
LifeSpan Biosciences
catalog: LS-C332459

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry

Western blot analysis of extracts of various cell lines.
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ACTN4 Antibody (aa27-38) LS-C345068
LifeSpan Biosciences
catalog: LS-C345068

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

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ACTN4 Antibody (aa1-269) LS-C372919
LifeSpan Biosciences
catalog: LS-C372919

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Knockout Varification: Lane 1: Wild-type MCF7 cell lysate; Lane 2: ACTN4 knockout MCF7 cell lysate; Predicted MW: 104,79,59kDa ; Observed MW: 104kDa; Primary Ab: 3µg/ml Rabbit Anti-Human ACTN4 Antibody; Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;

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