PDHX / Protein X / ProX Antibody (aa488-501) LS-C20201
LifeSpan Biosciences
catalog: LS-C20201

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry

(1 ug/ml) Staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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PDHX / Protein X / ProX Antibody (aa429-459) LS-C97695
LifeSpan Biosciences
catalog: LS-C97695

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of lysate from WiDr cell line, using hPDX1R444. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
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IHC-plus™ PDHX / Protein X / ProX Antibody (Thr11) LS-B9012
LifeSpan Biosciences
catalog: LS-B9012

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Fluorescent confocal image of SY5Y cells stained with PDX1 (T11) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated PDX1 (T11) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDX1 immunosignal to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000110435).

Western blot of PDX1 (arrow) using PDX1 Antibody (T11). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PDHX / Protein X / ProX Antibody LS-C334709
LifeSpan Biosciences
catalog: LS-C334709

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of U2OS cells.
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PDHX / Protein X / ProX Antibody (aa220-468) LS-C374911
LifeSpan Biosciences
catalog: LS-C374911

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Lane1: Human A431 Cells; Lane2: Human Hela Cells; Lane3: Porcine Heart Tissue.

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PDHX / Protein X / ProX Antibody (aa222-471) LS-C374914
LifeSpan Biosciences
catalog: LS-C374914

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of PDHX / Protein X / ProX antibody.

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PDHX / Protein X / ProX Antibody (aa1-300) LS-C376290
LifeSpan Biosciences
catalog: LS-C376290

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: PDHX antibody at 6 ug/ml. Lane 1: 293T whole cell lysate. Lane 2: PC-3 whole cell lysate. Lane 3: MCF-7 whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 54 kDa. Observed band size: 54 kDa Immunohistochemistry.

Immunohistochemistry of paraffin-embedded human placenta using antibody at 1:100 dilution.
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