CALM1 / Calmodulin Antibody (aa117-149) LS-C97762
LifeSpan Biosciences
catalog: LS-C97762

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-Calmodulin Antibody in HeLa cell line lysates (35 ug/lane). Calmodulin(arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human brain tissue reacted with Calmodulin antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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CALM2 / Calmodulin 2 Antibody (clone 3F4-G5) LS-C196807
LifeSpan Biosciences
catalog: LS-C196807

mouse monoclonal (3F4-G5)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Immunofluorescence of monoclonal antibody to CALM2 on HeLa cell . [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged CALM2 is approximately 0.1 ng/ml as a capture antibody.
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CALM3 / Calmodulin 3 Antibody (clone 1E2) LS-C196808
LifeSpan Biosciences
catalog: LS-C196808

mouse monoclonal (1E2)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged CALM3 is 0.03 ng/ml as a capture antibody.
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CALM1 / Calmodulin Antibody (aa1-149) LS-C293116
LifeSpan Biosciences
catalog: LS-C293116

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant CALM1 / Calmodulin. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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CALM1 / Calmodulin Antibody (aa1-149, Biotin) LS-C299231
LifeSpan Biosciences
catalog: LS-C299231

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western blot of recombinant CALM1 / Calmodulin. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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CALM3 / Calmodulin 3 Antibody LS-C306702
LifeSpan Biosciences
catalog: LS-C306702

mouse polyclonal
reactivity: human
application: western blot

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CALM1 / Calmodulin Antibody LS-C331329
LifeSpan Biosciences
catalog: LS-C331329

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using CALM1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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CALM3 / Calmodulin 3 Antibody LS-C335131
LifeSpan Biosciences
catalog: LS-C335131

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of mouse brain, using CALM3 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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CALM1 / Calmodulin Antibody (Internal) LS-C351892
LifeSpan Biosciences
catalog: LS-C351892

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of Calmodulin expression in HeLa (A); MCF7 (B); Ramos (C); NIH3T3 (D) whole cell lysates.

Immunohistochemical analysis of Calmodulin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Calmodulin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CALM1 / Calmodulin Antibody (phospho-Thr80/Ser82) LS-C354566
LifeSpan Biosciences
catalog: LS-C354566

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Calmodulin (pT80/S82) expression in MCF7 TNF-treated (A); NIH3T3 TNF-treated (B) whole cell lysates.

Immunohistochemical analysis of Calmodulin (pT80/S82) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w

Immunofluorescent analysis of Calmodulin (pT80/S82) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CALM1 / Calmodulin Antibody (aa1-149) LS-C374727
LifeSpan Biosciences
catalog: LS-C374727

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Recombinant CALM1, Human.

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CALM1 / Calmodulin Antibody (HRP) LS-C375371
LifeSpan Biosciences
catalog: LS-C375371

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA

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CALM1 / Calmodulin Antibody (FITC) LS-C375374
LifeSpan Biosciences
catalog: LS-C375374

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA

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CALM1 / Calmodulin Antibody (Biotin) LS-C375376
LifeSpan Biosciences
catalog: LS-C375376

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA

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