BRD3 Antibody (N-Terminus) LS-C31695
LifeSpan Biosciences
catalog: LS-C31695
domestic rabbit polyclonal
reactivity: human
application: western blot
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BRD3 Antibody LS-C77294
LifeSpan Biosciences
catalog: LS-C77294
mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
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BRD3 Antibody (aa647-696) LS-C110336
LifeSpan Biosciences
catalog: LS-C110336
domestic rabbit polyclonal
reactivity: human
application: western blot
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BRD3 Antibody (aa611-660) LS-C119051
LifeSpan Biosciences
catalog: LS-C119051
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from A549 cells, using BRD3 Antibody. The lane on the right is blocked with the synthesized peptide.
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BRD3 Antibody (clone 6E7) LS-C133529
LifeSpan Biosciences
catalog: LS-C133529
mouse monoclonal (6E7)
reactivity: human
application: western blot, ELISA

BRD3 monoclonal antibody (M01), clone 6E7 Western blot of BRD3 expression in HeLa NE.

Detection limit for recombinant GST tagged BRD3 is approximately 0.3 ng/ml as a capture antibody.
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BRD3 Antibody (clone 7G6) LS-C133530
LifeSpan Biosciences
catalog: LS-C133530
mouse monoclonal (7G6)
reactivity: human
application: western blot, ELISA

BRD3 monoclonal antibody (M02), clone 7G6 Western blot of BRD3 expression in HeLa.

Detection limit for recombinant GST tagged BRD3 is approximately 0.3 ng/ml as a capture antibody.
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BRD3 Antibody (clone 6C10) LS-C133531
LifeSpan Biosciences
catalog: LS-C133531
mouse monoclonal (6C10)
reactivity: human
application: western blot, ELISA

BRD3 monoclonal antibody (M03), clone 6C10. Western blot of BRD3 expression in human kidney.
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BRD3 Antibody (clone 6F3) LS-C133532
LifeSpan Biosciences
catalog: LS-C133532
mouse monoclonal (6F3)
reactivity: human
application: western blot, ELISA

BRD3 monoclonal antibody (M04), clone 6F3 Western blot of BRD3 expression in HeLa NE.

Detection limit for recombinant GST tagged BRD3 is approximately 0.1 ng/ml as a capture antibody.
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IHC-plus™ BRD3 Antibody (aa648-697) LS-B6601
LifeSpan Biosciences
catalog: LS-B6601
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-BRD3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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BRD3 Antibody (clone 2088C3a) LS-C184334
LifeSpan Biosciences
catalog: LS-C184334
mouse monoclonal (2088C3a)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
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BRD3 Antibody (Phe645) LS-C191980
LifeSpan Biosciences
catalog: LS-C191980
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry
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BRD3 Antibody (aa451-500) LS-C201275
LifeSpan Biosciences
catalog: LS-C201275
domestic rabbit polyclonal
reactivity: human
application: western blot
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BRD3 Antibody (aa672-702, AP) LS-C275687
LifeSpan Biosciences
catalog: LS-C275687
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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BRD3 Antibody (aa672-702, APC) LS-C275690
LifeSpan Biosciences
catalog: LS-C275690
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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BRD3 Antibody (aa672-702, FITC) LS-C275691
LifeSpan Biosciences
catalog: LS-C275691
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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BRD3 Antibody (aa672-702, Biotin) LS-C275692
LifeSpan Biosciences
catalog: LS-C275692
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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BRD3 Antibody (aa672-702, Azide-free, HRP) LS-C275693
LifeSpan Biosciences
catalog: LS-C275693
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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BRD3 Antibody (aa672-702, PE) LS-C275694
LifeSpan Biosciences
catalog: LS-C275694
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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IHC-plus™ BRD3 Antibody (aa672-702) LS-B10710
LifeSpan Biosciences
catalog: LS-B10710
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-BRD3 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

The anti-BRD3 antibody is used in Western blot to detect BRD3 in mouse brain tissue lysate (Lane 1) and Saos-2 cell lysate (Lane 2).
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BRD3 Antibody (aa676-726) LS-C286872
LifeSpan Biosciences
catalog: LS-C286872
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section
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BRD3 Antibody (aa275-325) LS-C289118
LifeSpan Biosciences
catalog: LS-C289118
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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BRD3 Antibody (aa676-726) LS-C289119
LifeSpan Biosciences
catalog: LS-C289119
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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BRD3 Antibody (N-Terminus) LS-C305978
LifeSpan Biosciences
catalog: LS-C305978
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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BRD3 Antibody LS-C309629
LifeSpan Biosciences
catalog: LS-C309629
domestic rabbit polyclonal
reactivity: human
application: western blot
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BRD3 Antibody (aa672-702) LS-C326966
LifeSpan Biosciences
catalog: LS-C326966
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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BRD3 Antibody (clone 2088C3a) LS-C342388
LifeSpan Biosciences
catalog: LS-C342388
mouse monoclonal (2088C3a)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of immunized recombinant protein, using anti-BRD3 monoclonal antibody.

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-BRD3 mAb. Predicted molecular weight: 79 kDa

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BRD3 mAb.
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BRD3 Antibody LS-C347575
LifeSpan Biosciences
catalog: LS-C347575
domestic rabbit polyclonal
reactivity: human
application: western blot
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BRD3 Antibody LS-C347577
LifeSpan Biosciences
catalog: LS-C347577
domestic rabbit polyclonal
reactivity: human
application: western blot
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BRD3 Antibody (C-Terminus) LS-C353038
LifeSpan Biosciences
catalog: LS-C353038
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of BRD3 expression in Saos2 (A); HepG2 (B) whole cell lysates.

Immunohistochemical analysis of BRD3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of BRD3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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BRD3 Antibody LS-C356836
LifeSpan Biosciences
catalog: LS-C356836
domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-BRD3 Antibody at 1:1000 dilution + A549 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 80 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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BRD3 Antibody (580-660 aa, C-terminal) LS-C382317
LifeSpan Biosciences
catalog: LS-C382317
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of BRD3 antibody
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