PathPlus™ NANOG Antibody (aa94-123) LS-B3425
LifeSpan Biosciences
catalog: LS-B3425

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-NANOG antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

NANOG Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The NANOG antibody detected the NANOG protein (arrow).

Immunofluorescence of NANOG Antibody with HeLa cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
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NANOG Antibody (Biotin) LS-C37434
LifeSpan Biosciences
catalog: LS-C37434

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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NANOG Antibody (aa144-157) LS-C55142
LifeSpan Biosciences
catalog: LS-C55142

domestic goat polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

NANOG antibody (0.03µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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NANOG Antibody (aa21-70) LS-C81137
LifeSpan Biosciences
catalog: LS-C81137

domestic rabbit polyclonal
reactivity: human
application: western blot

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NANOG Antibody (aa1-154, clone 5A10) LS-C93768
LifeSpan Biosciences
catalog: LS-C93768

mouse monoclonal (5A10)
reactivity: human
application: western blot, ELISA

The cell lysates of NIH3T3 (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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NANOG Antibody (aa1-50) LS-C19389
LifeSpan Biosciences
catalog: LS-C19389

domestic rabbit polyclonal
reactivity: human
application: western blot

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NANOG Antibody (aa24-73) LS-C30739
LifeSpan Biosciences
catalog: LS-C30739

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ NANOG Antibody (aa291-304) LS-B3017
LifeSpan Biosciences
catalog: LS-B3017

domestic goat polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Antibody (0.05 ug/ml) staining of HeLa (nuclear) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-NANOG antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
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NANOG Antibody (aa15-49) LS-C98243
LifeSpan Biosciences
catalog: LS-C98243

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of anti-NANOG Antibody in K562 cell line lysates (35 ug/lane). NANOG (arrow) was detected using the purified antibody.Western blot of NANOG (arrow) using rabbit polyclonal NANOG Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).

Fluorescent confocal image of SY5Y cells stained NANOG antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated NANOG primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.

Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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NANOG Antibody (Ser285) LS-C98246
LifeSpan Biosciences
catalog: LS-C98246

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of NANOG (arrow) using rabbit NANOG Antibody (S285). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).

Fluorescent confocal image of SY5Y cells stained NANOG (S285) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated NANOG (S285) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
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NANOG Antibody (Biotin) LS-C104594
LifeSpan Biosciences
catalog: LS-C104594

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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NANOG Antibody LS-C108536
LifeSpan Biosciences
catalog: LS-C108536

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of NANOG in human spleen tissue lysate with NANOG antibody at (A) 1 and (B) 2 ug/ml.

Immunofluorescence of NANOG in Human Spleen cells with NANOG antibody at 20 ug/ml.

Immunohistochemistry of NANOG in human spleen tissue with NANOG antibody at 5 ug/ml.
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NANOG Antibody (clone 1E6C4) LS-C108845
LifeSpan Biosciences
catalog: LS-C108845

mouse monoclonal (1E6C4)
reactivity: human
application: western blot, ELISA, immunocytochemistry

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NANOG Antibody LS-C109328
LifeSpan Biosciences
catalog: LS-C109328

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Sample (20 ug ). A: HeLa nucleus. 12% SDS PAGE. NANOG antibody diluted at 1:3000.
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NANOG Antibody (aa35-84) LS-C112347
LifeSpan Biosciences
catalog: LS-C112347

domestic rabbit polyclonal
reactivity: human
application: western blot

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NANOG Antibody (aa51-100) LS-C120045
LifeSpan Biosciences
catalog: LS-C120045

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HUVEC, HT-29, HepG2, and Jurkat cells, using NANOGP8 Antibody. The lane on the right is blocked with the synthesized peptide.
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NANOG Antibody (aa1-154) LS-C124221
LifeSpan Biosciences
catalog: LS-C124221

mouse monoclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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NANOG Antibody LS-C130968
LifeSpan Biosciences
catalog: LS-C130968

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry

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NANOG Antibody (aa20-166) LS-C130969
LifeSpan Biosciences
catalog: LS-C130969

mouse monoclonal
reactivity: human
application: western blot, ELISA

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NANOG Antibody LS-C130970
LifeSpan Biosciences
catalog: LS-C130970

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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NANOG Antibody (aa135-184) LS-C134918
LifeSpan Biosciences
catalog: LS-C134918

domestic rabbit polyclonal
reactivity: human
application: western blot

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NANOG Antibody LS-C146885
LifeSpan Biosciences
catalog: LS-C146885

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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NANOG Antibody (C-Terminus) LS-C153465
LifeSpan Biosciences
catalog: LS-C153465

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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IHC-plus™ NANOG Antibody LS-B8225
LifeSpan Biosciences
catalog: LS-B8225

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-NANOG antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
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NANOG Antibody (phospho-Ser71) LS-C163646
LifeSpan Biosciences
catalog: LS-C163646

domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry

Fluorescent confocal image of SY5Y cells stained Phospho-Nanog-S71 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated Phospho-Nanog-S71 primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Phospho-Nanog immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.

Dot blot of anti-Phospho-Nanog-S71 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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NANOG Antibody (phospho-Ser285) LS-C203687
LifeSpan Biosciences
catalog: LS-C203687

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated

Dot blot of anti-Phospho-Nanog-S285 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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NANOG Antibody (Ser285, AP) LS-C263946
LifeSpan Biosciences
catalog: LS-C263946

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunocytochemistry

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NANOG Antibody (Ser285, APC) LS-C263947
LifeSpan Biosciences
catalog: LS-C263947

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunocytochemistry

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NANOG Antibody (Ser285, FITC) LS-C263948
LifeSpan Biosciences
catalog: LS-C263948

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunocytochemistry

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NANOG Antibody (Ser285, Azide-free, HRP) LS-C263949
LifeSpan Biosciences
catalog: LS-C263949

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunocytochemistry

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NANOG Antibody (Ser285, PE) LS-C263950
LifeSpan Biosciences
catalog: LS-C263950

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunocytochemistry

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NANOG Antibody (aa94-123, AP) LS-C263951
LifeSpan Biosciences
catalog: LS-C263951

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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NANOG Antibody (aa94-123, APC) LS-C263952
LifeSpan Biosciences
catalog: LS-C263952

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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NANOG Antibody (aa94-123, FITC) LS-C263953
LifeSpan Biosciences
catalog: LS-C263953

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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NANOG Antibody (aa94-123, Azide-free, HRP) LS-C263954
LifeSpan Biosciences
catalog: LS-C263954

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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NANOG Antibody (aa94-123, PE) LS-C263957
LifeSpan Biosciences
catalog: LS-C263957

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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NANOG Antibody (phospho-Ser71, AP) LS-C279943
LifeSpan Biosciences
catalog: LS-C279943

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA

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NANOG Antibody (phospho-Ser71, APC) LS-C279944
LifeSpan Biosciences
catalog: LS-C279944

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA

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NANOG Antibody (phospho-Ser71, FITC) LS-C279945
LifeSpan Biosciences
catalog: LS-C279945

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA

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NANOG Antibody (phospho-Ser71, Azide-free, HRP) LS-C279946
LifeSpan Biosciences
catalog: LS-C279946

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA

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NANOG Antibody (phospho-Ser71, PE) LS-C279947
LifeSpan Biosciences
catalog: LS-C279947

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA

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NANOG Antibody (phospho-Ser285, AP) LS-C279948
LifeSpan Biosciences
catalog: LS-C279948

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA

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NANOG Antibody (phospho-Ser285, APC) LS-C279949
LifeSpan Biosciences
catalog: LS-C279949

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA

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NANOG Antibody (phospho-Ser285, FITC) LS-C279950
LifeSpan Biosciences
catalog: LS-C279950

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA

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NANOG Antibody (phospho-Ser285, Azide-free, HRP) LS-C279951
LifeSpan Biosciences
catalog: LS-C279951

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA

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NANOG Antibody (phospho-Ser285, PE) LS-C279952
LifeSpan Biosciences
catalog: LS-C279952

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA

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NANOG Antibody (aa1-50) LS-C286456
LifeSpan Biosciences
catalog: LS-C286456

domestic rabbit polyclonal
reactivity: human, mouse
application: immunohistochemistry

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NANOG Antibody (aa250-C-term) LS-C287289
LifeSpan Biosciences
catalog: LS-C287289

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunoprecipitation

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NANOG Antibody (aa1-50) LS-C287290
LifeSpan Biosciences
catalog: LS-C287290

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunoprecipitation

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NANOG Antibody (Ser285) LS-C323224
LifeSpan Biosciences
catalog: LS-C323224

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

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NANOG Antibody (aa94-123) LS-C323228
LifeSpan Biosciences
catalog: LS-C323228

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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NANOG Antibody (phospho-Ser71) LS-C327996
LifeSpan Biosciences
catalog: LS-C327996

domestic rabbit polyclonal
reactivity: human
application: ELISA

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NANOG Antibody (phospho-Ser285) LS-C327997
LifeSpan Biosciences
catalog: LS-C327997

domestic rabbit polyclonal
reactivity: human
application: ELISA

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NANOG Antibody (clone 2C4) LS-C348865
LifeSpan Biosciences
catalog: LS-C348865

mouse monoclonal (2C4)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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NANOG Antibody (aa155-189) LS-C356626
LifeSpan Biosciences
catalog: LS-C356626

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-NANOG Antibody at 1:2000 dilution + human brain lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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NANOG Antibody (aa20-166, clone 1E6C4) LS-C359267
LifeSpan Biosciences
catalog: LS-C359267

mouse monoclonal (1E6C4)
reactivity: human
application: western blot, ELISA, immunocytochemistry

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NANOG Antibody (aa1-305) LS-C374979
LifeSpan Biosciences
catalog: LS-C374979

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Lane1: Human Umbilical Cord Tissue; Lane2: Human Placenta Tissue.

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NANOG Antibody (FITC) LS-C380164
LifeSpan Biosciences
catalog: LS-C380164

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA

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NANOG Antibody LS-C380166
LifeSpan Biosciences
catalog: LS-C380166

domestic rabbit polyclonal
reactivity: human
application: ELISA

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NANOG Antibody (HRP) LS-C380168
LifeSpan Biosciences
catalog: LS-C380168

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA

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NANOG Antibody (Biotin) LS-C380170
LifeSpan Biosciences
catalog: LS-C380170

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA

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