IHC-plus™ TUSC3 Antibody (Internal) LS-B13274
LifeSpan Biosciences
catalog: LS-B13274

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Western blot analysis of TUSC3 expression in SKBR3 (A); HeLa (B); HepG2 (C); MDAMB231 (D) whole cell lysates.

Immunohistochemical analysis of TUSC3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of TUSC3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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TUSC3 Antibody (100-180 aa, Internal) LS-C384735
LifeSpan Biosciences
catalog: LS-C384735

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 1

Western blot of N33 antibody
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TUSC3 Antibody (aa95-106) LS-C61799
LifeSpan Biosciences
catalog: LS-C61799

domestic goat polyclonal
reactivity: human
application: western blot

Antibody (1 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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TUSC3 Antibody (aa131-180) LS-C119019
LifeSpan Biosciences
catalog: LS-C119019

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells using TUSC3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO205 and HeLa cells, using TUSC3 Antibody. The lane on the right is blocked with the synthesized peptide.
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TUSC3 Antibody (His153) LS-C191974
LifeSpan Biosciences
catalog: LS-C191974

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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TUSC3 Antibody (N-Terminus) LS-C346040
LifeSpan Biosciences
catalog: LS-C346040

domestic rabbit polyclonal
reactivity: human
application: western blot

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TUSC3 Antibody LS-C357012
LifeSpan Biosciences
catalog: LS-C357012

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-TUSC3 Antibody at 1:1000 dilution + HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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TUSC3 Antibody LS-C416198
LifeSpan Biosciences
catalog: LS-C416198

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of COLO205 cell lysate using TUSC3 Antibody
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