LIN28A / LIN28 Antibody (clone 6D1F9) LS-C108834
LifeSpan Biosciences
catalog: LS-C108834

mouse monoclonal (6D1F9)
reactivity: human
application: western blot, ELISA, immunocytochemistry

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IHC-plus™ LIN28A / LIN28 Antibody (N-Terminus) LS-B3510
LifeSpan Biosciences
catalog: LS-B3510

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-LIN28A / LIN28 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


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IHC-plus™ LIN28A / LIN28 Antibody LS-B5073
LifeSpan Biosciences
catalog: LS-B5073

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-LIN28A / LIN28 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of LIN28 in human spleen tissue with LIN28 antibody at 20 ug/ml.

Western blot analysis of Lin28 in Raji cell lysate with Lin28 antibody at (A) 1 and (B) 2 ug/ml.
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IHC-plus™ LIN28A / LIN28 Antibody (aa108-138) LS-B11566
LifeSpan Biosciences
catalog: LS-B11566

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 1

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Fluorescent confocal image of SY5Y cells stained LIN28A antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated LIN28A primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Lin28a immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.

LIN28A Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The LIN28A antibody detected the LIN28A protein (arrow).
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LIN28A / LIN28 Antibody (clone 55CT58.12.1) LS-C96465
LifeSpan Biosciences
catalog: LS-C96465

mouse monoclonal (55CT58.12.1)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of LIN28 recombinant protein by anti-LIN28A Monoclonal Antibody. LIN28 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28A Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence of LIN28A Monoclonal Antibody with paraffin-embedded human testis tissue. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.
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LIN28A / LIN28 Antibody LS-C125874
LifeSpan Biosciences
catalog: LS-C125874

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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LIN28A / LIN28 Antibody LS-C146851
LifeSpan Biosciences
catalog: LS-C146851

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

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LIN28A / LIN28 Antibody (aa100-150) LS-C148680
LifeSpan Biosciences
catalog: LS-C148680

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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LIN28A / LIN28 Antibody (phospho-Ser134) LS-C163637
LifeSpan Biosciences
catalog: LS-C163637

domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry

Fluorescent confocal image of SY5Y cells stained with phospho- LIN28- S134 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho- LIN28- S134 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho- LIN28 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000131914).

Dot blot of anti-Phospho-LIN28-S134 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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LIN28A / LIN28 Antibody (aa1-209) LS-C178747
LifeSpan Biosciences
catalog: LS-C178747

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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LIN28A / LIN28 Antibody LS-C180090
LifeSpan Biosciences
catalog: LS-C180090

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

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LIN28A / LIN28 Antibody LS-C203674
LifeSpan Biosciences
catalog: LS-C203674

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

LIN28 Antibody western blot of MDA-MB435 cell line lysates (35 ug/lane). The LIN28 antibody detected the LIN28 protein (arrow).

LIN28 Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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LIN28A / LIN28 Antibody (clone 55CT58.12.1, AP) LS-C221300
LifeSpan Biosciences
catalog: LS-C221300

mouse monoclonal (55CT58.12.1)
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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LIN28A / LIN28 Antibody (clone 55CT58.12.1, APC) LS-C221301
LifeSpan Biosciences
catalog: LS-C221301

mouse monoclonal (55CT58.12.1)
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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LIN28A / LIN28 Antibody (clone 55CT58.12.1, FITC) LS-C221302
LifeSpan Biosciences
catalog: LS-C221302

mouse monoclonal (55CT58.12.1)
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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LIN28A / LIN28 Antibody (clone 55CT58.12.1, Biotin) LS-C221303
LifeSpan Biosciences
catalog: LS-C221303

mouse monoclonal (55CT58.12.1)
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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LIN28A / LIN28 Antibody (clone 55CT58.12.1, Azide-free, HRP) LS-C221305
LifeSpan Biosciences
catalog: LS-C221305

mouse monoclonal (55CT58.12.1)
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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LIN28A / LIN28 Antibody (clone 55CT58.12.1, PE) LS-C221306
LifeSpan Biosciences
catalog: LS-C221306

mouse monoclonal (55CT58.12.1)
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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LIN28A / LIN28 Antibody (phospho-Ser134, AP) LS-C226832
LifeSpan Biosciences
catalog: LS-C226832

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunocytochemistry

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LIN28A / LIN28 Antibody (phospho-Ser134, APC) LS-C226833
LifeSpan Biosciences
catalog: LS-C226833

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunocytochemistry

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LIN28A / LIN28 Antibody (phospho-Ser134, Biotin) LS-C226834
LifeSpan Biosciences
catalog: LS-C226834

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunocytochemistry

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LIN28A / LIN28 Antibody (phospho-Ser134, FITC) LS-C226835
LifeSpan Biosciences
catalog: LS-C226835

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunocytochemistry

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LIN28A / LIN28 Antibody (phospho-Ser134, Azide-free, HRP) LS-C226837
LifeSpan Biosciences
catalog: LS-C226837

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunocytochemistry

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LIN28A / LIN28 Antibody (phospho-Ser134, PE) LS-C226839
LifeSpan Biosciences
catalog: LS-C226839

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunocytochemistry

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LIN28A / LIN28 Antibody LS-C308759
LifeSpan Biosciences
catalog: LS-C308759

mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry

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LIN28A / LIN28 Antibody LS-C310877
LifeSpan Biosciences
catalog: LS-C310877

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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LIN28A / LIN28 Antibody LS-C310881
LifeSpan Biosciences
catalog: LS-C310881

domestic rabbit polyclonal
reactivity: human
application: western blot

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LIN28A / LIN28 Antibody (clone 55CT58.12.1) LS-C319169
LifeSpan Biosciences
catalog: LS-C319169

mouse monoclonal (55CT58.12.1)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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LIN28A / LIN28 Antibody (phospho-Ser134) LS-C322664
LifeSpan Biosciences
catalog: LS-C322664

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunocytochemistry

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LIN28A / LIN28 Antibody LS-C334475
LifeSpan Biosciences
catalog: LS-C334475

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of various cell lines.
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LIN28A / LIN28 Antibody LS-C342036
LifeSpan Biosciences
catalog: LS-C342036

mouse monoclonal
reactivity: human
application: western blot

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LIN28A / LIN28 Antibody (aa22-56) LS-C344461
LifeSpan Biosciences
catalog: LS-C344461

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysate from NCCIT cell line with LIN28A Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
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LIN28A / LIN28 Antibody (clone 2F11) LS-C348862
LifeSpan Biosciences
catalog: LS-C348862

mouse monoclonal (2F11)
reactivity: human
application: western blot, ELISA, immunohistochemistry

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LIN28A / LIN28 Antibody (aa177-210) LS-C356536
LifeSpan Biosciences
catalog: LS-C356536

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Anti-Lin28a Antibody at 1:2000 dilution + F9 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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