IHC-plus™ PAX8 Antibody (aa2-14) LS-B2
LifeSpan Biosciences
catalog: LS-B2

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PAX8 antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

PAX8 antibody (1µg/ml) staining of Human Kidney (A) and HeLa cell (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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PAX8 Antibody (aa189-200) LS-C55241
LifeSpan Biosciences
catalog: LS-C55241

domestic goat polyclonal
reactivity: human
application: western blot

HEK293 overexpressing PAX8A (RC200651) and probed with (mock transfection in first lane).
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PAX8 Antibody (C-Terminus) LS-C74178
LifeSpan Biosciences
catalog: LS-C74178

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

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IHC-plus™ PAX8 Antibody (aa215-264) LS-B5611
LifeSpan Biosciences
catalog: LS-B5611

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PAX8 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PAX8 Antibody (aa347-396) LS-C145536
LifeSpan Biosciences
catalog: LS-C145536

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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PAX8 Antibody (clone 2F1) LS-C139507
LifeSpan Biosciences
catalog: LS-C139507

mouse monoclonal (2F1)
reactivity: human
application: western blot, ELISA

Western blot of PAX8 expression in transfected 293T cell line by PAX8 monoclonal antibody (M23), clone 2F1.
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PAX8 Antibody (aa285-313) LS-C163398
LifeSpan Biosciences
catalog: LS-C163398

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of PAX8 Antibody in HL-60 cell line lysates (35 ug/lane). PAX8 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded mouse kidney reacted with PAX8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric of 293 cells using PAX8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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PAX8 Antibody (clone 2D4) LS-C169268
LifeSpan Biosciences
catalog: LS-C169268

mouse monoclonal (2D4)
reactivity: human
application: western blot, ELISA

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PAX8 Antibody (clone PAX8R1) LS-C184295
LifeSpan Biosciences
catalog: LS-C184295

mouse monoclonal (PAX8R1)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

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PAX8 Antibody (aa1-204) LS-C185416
LifeSpan Biosciences
catalog: LS-C185416

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Sample (30 ug whole cell lysate). A:293T. 10% SDS PAGE. PAX8 antibody diluted at 1:1000

Immunofluorescence of paraformaldehyde-fixed A549 using PAX8 antibody at 1:200 dilution.

PAX8 antibody detects PAX8 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. PAX8 antibody dilution:1:500.
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PAX8 Antibody (aa279-328) LS-C186653
LifeSpan Biosciences
catalog: LS-C186653

domestic rabbit polyclonal
reactivity: human
application: western blot

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PAX8 Antibody (aa180-229) LS-C201545
LifeSpan Biosciences
catalog: LS-C201545

domestic rabbit polyclonal
reactivity: human
application: western blot

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PAX8 Antibody (aa285-313, AP) LS-C280580
LifeSpan Biosciences
catalog: LS-C280580

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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PAX8 Antibody (aa285-313, APC) LS-C280582
LifeSpan Biosciences
catalog: LS-C280582

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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PAX8 Antibody (aa285-313, Biotin) LS-C280583
LifeSpan Biosciences
catalog: LS-C280583

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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PAX8 Antibody (aa285-313, FITC) LS-C280584
LifeSpan Biosciences
catalog: LS-C280584

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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PAX8 Antibody (aa285-313, Azide-free, HRP) LS-C280585
LifeSpan Biosciences
catalog: LS-C280585

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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PAX8 Antibody (aa285-313, PE) LS-C280586
LifeSpan Biosciences
catalog: LS-C280586

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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PAX8 Antibody (clone ZR-1) LS-C312140
LifeSpan Biosciences
catalog: LS-C312140

domestic rabbit recombinant (ZR-1)
reactivity: human
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of PAX-8 on FFPE Ovarian Carcinoma tissue.
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PAX8 Antibody (aa285-313) LS-C328158
LifeSpan Biosciences
catalog: LS-C328158

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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PAX8 Antibody LS-C331202
LifeSpan Biosciences
catalog: LS-C331202

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of extracts of SKOV3 cells, using PAX8 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunofluorescence analysis of U2OS cells using PAX8 antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.
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PAX8 Antibody (clone PAX8R1) LS-C342350
LifeSpan Biosciences
catalog: LS-C342350

mouse monoclonal (PAX8R1)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of immunized recombinant protein, using anti-PAX8 monoclonal antibody.

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-PAX8 mAb. Predicted molecular weight: 48 kDa

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PAX8 mAb.
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IHC-plus™ PAX8 Antibody (aa151-200) LS-B11938
LifeSpan Biosciences
catalog: LS-B11938

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PAX8 Antibody (clone ZR-1, Ready-to-Use) LS-C348564
LifeSpan Biosciences
catalog: LS-C348564

domestic rabbit recombinant (ZR-1)
reactivity: human
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of PAX-8 on FFPE Ovarian Carcinoma tissue. This image was taken for the unmodified form of this product. Other forms have not been tested.
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PAX8 Antibody (Internal) LS-C353030
LifeSpan Biosciences
catalog: LS-C353030

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of PAX8 expression in HeLa (A); HEK293T (B) whole cell lysates.

Immunohistochemical analysis of PAX8 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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IHC-plus™ PAX8 Antibody (aa145-194) LS-B12565
LifeSpan Biosciences
catalog: LS-B12565

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot analysis of lysate from HT-29 cells, using Pax-8 antibody.
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PAX8 Antibody (Internal) LS-C385372
LifeSpan Biosciences
catalog: LS-C385372

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Pax-8 antibody
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IHC-plus™ PAX8 Antibody (clone 1F8-3A8) LS-B13466
LifeSpan Biosciences
catalog: LS-B13466

mouse monoclonal (1F8-3A8)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
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