sPLA2 Antibody LS-C17272
LifeSpan Biosciences
catalog: LS-C17272

mouse monoclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
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YWHAZ / 14-3-3 Zeta Antibody (phospho-Ser184) LS-C145103
LifeSpan Biosciences
catalog: LS-C145103

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (C-Terminus) LS-C81147
LifeSpan Biosciences
catalog: LS-C81147

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (phospho-Ser58) LS-C92056
LifeSpan Biosciences
catalog: LS-C92056

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa216-245) LS-C101299
LifeSpan Biosciences
catalog: LS-C101299

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with 14-3-3 protein zeta/delta antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot of hYWHAZ-D231 in mouse brain tissue and MCF-7 cell line lysates (35 ug/lane). YWHAZ (arrow) was detected using the purified antibody.
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YWHAZ / 14-3-3 Zeta Antibody (Thr232) LS-C101300
LifeSpan Biosciences
catalog: LS-C101300

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-14-3-3 protein zeta/delta Antibody (T232) in A375 cell line lysates (35 ug/lane). 14-3-3 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human testis tissue reacted with YWHAZ Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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price:
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YWHAZ / 14-3-3 Zeta Antibody (aa1-10) LS-C121323
LifeSpan Biosciences
catalog: LS-C121323

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody LS-C122565
LifeSpan Biosciences
catalog: LS-C122565

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

quantity:
price:
to the supplier

sPLA2-IB Antibody LS-C131405
LifeSpan Biosciences
catalog: LS-C131405

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

sPLA2-IIA Antibody LS-C131406
LifeSpan Biosciences
catalog: LS-C131406

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

sPLA2-IIE Antibody LS-C131407
LifeSpan Biosciences
catalog: LS-C131407

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

sPLA2-IID Antibody LS-C131408
LifeSpan Biosciences
catalog: LS-C131408

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

sPLA2-XII Antibody LS-C131409
LifeSpan Biosciences
catalog: LS-C131409

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

sPLA2-X Antibody LS-C131410
LifeSpan Biosciences
catalog: LS-C131410

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

IHC-plus™ YWHAZ / 14-3-3 Zeta Antibody (C-Terminus) LS-B5807
LifeSpan Biosciences
catalog: LS-B5807

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-YWHAZ / 14-3-3 Zeta Delta antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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YWHAZ / 14-3-3 Zeta Antibody (aa50-100) LS-C148660
LifeSpan Biosciences
catalog: LS-C148660

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity:
price:
to the supplier

IHC-plus™ YWHAZ / 14-3-3 Zeta Antibody (aa196-245) LS-B7637
LifeSpan Biosciences
catalog: LS-B7637

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-YWHAZ / 14-3-3 Zeta Delta antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of NIH/3T3 cells, using 14-3-3 zeta/delta Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from K562 cells, using 14-3-3 zeta/delta Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ YWHAZ / 14-3-3 Zeta Antibody (aa51-100) LS-B7801
LifeSpan Biosciences
catalog: LS-B7801

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-14-3-3 Zeta antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.

Western blot analysis of lysates from HeLa, Jurkat, and COLO cells, using 14-3-3 zeta Antibody. The lane on the right is blocked with the synthesized peptide.
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YWHAZ / 14-3-3 Zeta Antibody LS-C153411
LifeSpan Biosciences
catalog: LS-C153411

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa65-93) LS-C160705
LifeSpan Biosciences
catalog: LS-C160705

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of YWHAZ Antibody in MDA-MB231, A2058 cell line and mouse cerebellum, brain tissue lysates (35 ug/lane). YWHAZ (arrow) was detected using the purified antibody.

YWHAZ Antibody IHC of formalin-fixed and paraffin-embedded mouse brain followed by peroxidase-conjugated secondary antibody and DAB staining.

YWHAZ Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IHC-plus™ YWHAZ / 14-3-3 Zeta Antibody (aa200-245) LS-B8679
LifeSpan Biosciences
catalog: LS-B8679

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-14-3-3 Zeta antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
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price:
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YWHAZ / 14-3-3 Zeta Antibody (Val52) LS-C175907
LifeSpan Biosciences
catalog: LS-C175907

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot of 14-3-3 (V52) pAb in extracts from 293 Forskolin 40nM 30' NIH/3T3 PMA 250ng/ml 5'' cells.

IHC of 14-3-3 (V52) pAb in paraffin-embedded human breast carcinoma tissue.
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price:
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YWHAZ / 14-3-3 Zeta Antibody (Trp228) LS-C176663
LifeSpan Biosciences
catalog: LS-C176663

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of 14-3-3 / (W228) pAb in extracts from K562 cells.

IHC of 14-3-3 / (W228) pAb in paraffin-embedded human colon carcinoma tissue.
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YWHAZ / 14-3-3 Zeta Antibody (Ala79) LS-C176958
LifeSpan Biosciences
catalog: LS-C176958

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of 14-3-3 (A79) pAb pAb in extracts from HeLa cells.

IHC of 14-3-3 (A79) pAb in paraffin-embedded human brain tissue.
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price:
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YWHAZ / 14-3-3 Zeta Antibody (phospho-Ser58) LS-C192151
LifeSpan Biosciences
catalog: LS-C192151

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-14-3-3 zeta (S58) pAb in extracts from Jurkat cells treated with Insulin.

Immunohistochemistry (IHC) analysis of p-14-3-3 zeta (S58) pAb in paraffin-embedded human breast cancer tissue.
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YWHAZ / 14-3-3 Zeta Antibody (clone 1B3) LS-C198450
LifeSpan Biosciences
catalog: LS-C198450

mouse monoclonal (1B3)
reactivity: human
application: western blot, ELISA

Western Blot analysis of YWHAZ expression in transfected 293T cell line by YWHAZ monoclonal antibody (M04), clone 1B3.Lane 1: YWHAZ transfected lysate(27.7 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged YWHAZ is 0.03 ng/ml as a capture antibody.
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YWHAZ / 14-3-3 Zeta Antibody (aa65-93, AP) LS-C250053
LifeSpan Biosciences
catalog: LS-C250053

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa65-93, APC) LS-C250054
LifeSpan Biosciences
catalog: LS-C250054

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa65-93, Biotin) LS-C250055
LifeSpan Biosciences
catalog: LS-C250055

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa65-93, FITC) LS-C250056
LifeSpan Biosciences
catalog: LS-C250056

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa65-93, Azide-free, HRP) LS-C250057
LifeSpan Biosciences
catalog: LS-C250057

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa65-93, PE) LS-C250058
LifeSpan Biosciences
catalog: LS-C250058

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (Thr232, APC) LS-C250059
LifeSpan Biosciences
catalog: LS-C250059

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (Thr232, AP) LS-C250060
LifeSpan Biosciences
catalog: LS-C250060

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (Thr232, Biotin) LS-C250061
LifeSpan Biosciences
catalog: LS-C250061

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (Thr232, Azide-free, HRP) LS-C250062
LifeSpan Biosciences
catalog: LS-C250062

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (Thr232, FITC) LS-C250063
LifeSpan Biosciences
catalog: LS-C250063

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (Thr232, PE) LS-C250064
LifeSpan Biosciences
catalog: LS-C250064

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa216-245, AP) LS-C250065
LifeSpan Biosciences
catalog: LS-C250065

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa216-245, APC) LS-C250066
LifeSpan Biosciences
catalog: LS-C250066

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa216-245, Biotin) LS-C250067
LifeSpan Biosciences
catalog: LS-C250067

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa216-245, FITC) LS-C250068
LifeSpan Biosciences
catalog: LS-C250068

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa216-245, Azide-free, HRP) LS-C250069
LifeSpan Biosciences
catalog: LS-C250069

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa216-245, PE) LS-C250070
LifeSpan Biosciences
catalog: LS-C250070

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (phospho-Ser58) LS-C291151
LifeSpan Biosciences
catalog: LS-C291151

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.


Western blot analysis of lysates from NIH/3T3 cells treated with UV 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The lane on the right is blocked with the phospho peptide.
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YWHAZ / 14-3-3 Zeta Antibody (phospho-Thr232) LS-C291159
LifeSpan Biosciences
catalog: LS-C291159

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human brain, using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with UV 15', using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The lane on the right is blocked with the phospho peptide.
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YWHAZ / 14-3-3 Zeta Antibody (aa1-245) LS-C296898
LifeSpan Biosciences
catalog: LS-C296898

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant YWHAZ / 14-3-3 Zeta. This image was taken for the unconjugated form of this product. Other forms have not been tested.

quantity:
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YWHAZ / 14-3-3 Zeta Antibody (aa1-245, Biotin) LS-C298070
LifeSpan Biosciences
catalog: LS-C298070

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western blot of recombinant YWHAZ / 14-3-3 Zeta. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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YWHAZ / 14-3-3 Zeta Antibody (aa65-93) LS-C326762
LifeSpan Biosciences
catalog: LS-C326762

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (aa216-245) LS-C326765
LifeSpan Biosciences
catalog: LS-C326765

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (Thr232) LS-C330325
LifeSpan Biosciences
catalog: LS-C330325

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody LS-C330820
LifeSpan Biosciences
catalog: LS-C330820

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry

Western blot analysis of extracts of various cell lines, using YWHAZ antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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YWHAZ / 14-3-3 Zeta Antibody (phospho-Ser58) LS-C335862
LifeSpan Biosciences
catalog: LS-C335862

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts from Hela cells.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.

Immunofluorescence staining of methanol-fixed Hela cells.
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YWHAZ / 14-3-3 Zeta Antibody (clone 1314CT423.108.153.173.140) LS-C344132
LifeSpan Biosciences
catalog: LS-C344132

mouse monoclonal (1314CT423.108.153.173.140)
reactivity: human
application: western blot, immunocytochemistry

Fluorescent image of U251 cells stained with YWHAZ Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot of lysates from A431, HepG2, mouse NIH/3T3, rat PC-12 and C6 cell line, mouse brain tissue (from left to right) with YWHAZ Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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YWHAZ / 14-3-3 Zeta Antibody (phospho-Ser58) LS-C353022
LifeSpan Biosciences
catalog: LS-C353022

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of 14-3-3 zeta (pS58) expression in MCF7 (A); A549 (B); SP2/0 (C); rat liver (D); NIH3T3 (E); A431 (F) whole cell lysates.

Immunohistochemical analysis of 14-3-3 zeta (pS58) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of 14-3-3 zeta (pS58) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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YWHAZ / 14-3-3 Zeta Antibody (Internal) LS-C353025
LifeSpan Biosciences
catalog: LS-C353025

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of 14-3-3 zeta expression in HeLa (A); mouse brain (B); rat kidney (C) whole cell lysates.

Immunohistochemical analysis of 14-3-3 zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of 14-3-3 zeta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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YWHAZ / 14-3-3 Zeta Antibody (Internal) LS-C353026
LifeSpan Biosciences
catalog: LS-C353026

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

Western blot analysis of 14-3-3 zeta expression in KNRK (A); NIH3T3 (B); C4 (C); EOC20 (D); CTLL2 (E) whole cell lysates.
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YWHAZ / 14-3-3 Zeta Antibody (C-Terminus) LS-C354122
LifeSpan Biosciences
catalog: LS-C354122

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of 14-3-3 zeta expression in MCF7 (A); HeLa (B); SP2/0 (C); H9C2 (D) whole cell lysates.

Immunohistochemical analysis of 14-3-3 zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of 14-3-3 zeta staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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YWHAZ / 14-3-3 Zeta Antibody (aa48-245) LS-C358150
LifeSpan Biosciences
catalog: LS-C358150

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (C-Terminus) LS-C368806
LifeSpan Biosciences
catalog: LS-C368806

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of 14-3-3 zeta (pT232) expression in A431 UV-treated (A); K562 UV-treated (B); NIH3T3 UV-treated (C) whole cell lysates.

Immunohistochemical analysis of 14-3-3 zeta (pT232) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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price:
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YWHAZ / 14-3-3 Zeta Antibody (170-250 aa) LS-C381308
LifeSpan Biosciences
catalog: LS-C381308

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (40-120 aa) LS-C381449
LifeSpan Biosciences
catalog: LS-C381449

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Phospho-14-3-3 zeta (S58) antibody
quantity:
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to the supplier

YWHAZ / 14-3-3 Zeta Antibody (30-110 aa, Internal) LS-C381807
LifeSpan Biosciences
catalog: LS-C381807

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of 14-3-3 zeta antibody
quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (40-120 aa) LS-C381810
LifeSpan Biosciences
catalog: LS-C381810

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot of 14-3-3 zeta antibody
quantity:
price:
to the supplier

YWHAZ / 14-3-3 Zeta Antibody (170-250 aa) LS-C381811
LifeSpan Biosciences
catalog: LS-C381811

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

quantity:
price:
to the supplier