YWHAG / 14-3-3 Gamma Antibody (N-Terminus, clone HS23) LS-C2587
LifeSpan Biosciences
catalog: LS-C2587
mouse monoclonal (HS23)
reactivity: human
application: western blot, immunocytochemistry
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IHC-plus™ YWHAG / 14-3-3 Gamma Antibody (N-Terminus, clone KC21) LS-B213
LifeSpan Biosciences
catalog: LS-B213
mouse monoclonal (KC21)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-YWHAG / 14-3-3 Gamma antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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YWHAG / 14-3-3 Gamma Antibody (N-Terminus) LS-C63345
LifeSpan Biosciences
catalog: LS-C63345
mouse monoclonal
reactivity: human
application: western blot
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YWHAG / 14-3-3 Gamma Antibody (N-Terminus) LS-C63346
LifeSpan Biosciences
catalog: LS-C63346
mouse monoclonal
reactivity: human
application: western blot
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YWHAG / 14-3-3 Gamma Antibody LS-C109510
LifeSpan Biosciences
catalog: LS-C109510
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence of methanol-fixed HeLa, using 14-3-3 gamma antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate). A: 293T. B: A431. 12% SDS PAGE. YWHAG antibody diluted at 1:1000.
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YWHAG / 14-3-3 Gamma Antibody (aa51-100) LS-C119362
LifeSpan Biosciences
catalog: LS-C119362
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of COS7 cells, using 14-3-3 gamma Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using 14-3-3 gamma Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from K562 cells, treated with insulin 0.01U/ml 15', using 14-3-3 gamma Antibody. The lane on the right is blocked with the synthesized peptide.
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YWHAG / 14-3-3 Gamma Antibody (N-Terminus) LS-C124836
LifeSpan Biosciences
catalog: LS-C124836
mouse monoclonal
reactivity: human
application: western blot
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YWHAG / 14-3-3 Gamma Antibody (aa40-89) LS-C146074
LifeSpan Biosciences
catalog: LS-C146074
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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YWHAG / 14-3-3 Gamma Antibody (aa136-164) LS-C160699
LifeSpan Biosciences
catalog: LS-C160699
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of lysate from HeLa cell line, using YWHAG Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with YWHAG Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric of widr cells using YWHAG Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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YWHAG / 14-3-3 Gamma Antibody (Met81) LS-C177031
LifeSpan Biosciences
catalog: LS-C177031
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of 14-3-3 (M81) pAb in extracts from HeLa cells.

IHC of 14-3-3 (M81) pAb in paraffin-embedded human brain tissue.
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YWHAG / 14-3-3 Gamma Antibody (clone KC21) LS-C179727
LifeSpan Biosciences
catalog: LS-C179727
mouse monoclonal (KC21)
reactivity: human
application: western blot, immunoprecipitation
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YWHAG / 14-3-3 Gamma Antibody (clone 3G3) LS-C198448
LifeSpan Biosciences
catalog: LS-C198448
mouse monoclonal (3G3)
reactivity: human
application: ELISA

Detection limit for recombinant GST tagged YWHAG is approximately 0.3 ng/ml as a capture antibody.
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YWHAG / 14-3-3 Gamma Antibody (aa137-162) LS-C204047
LifeSpan Biosciences
catalog: LS-C204047
domestic rabbit polyclonal
reactivity: human
application: western blot

DANRE ywhag1 Antibody western blot of zebra fish brain tissue lysates (35 ug/lane). The DANRE ywhag1 antibody detected the DANRE ywhag1 protein (arrow).
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YWHAG / 14-3-3 Gamma Antibody (aa63-92, AP) LS-C232182
LifeSpan Biosciences
catalog: LS-C232182
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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YWHAG / 14-3-3 Gamma Antibody (aa63-92, APC) LS-C232183
LifeSpan Biosciences
catalog: LS-C232183
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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YWHAG / 14-3-3 Gamma Antibody (aa63-92, Biotin) LS-C232184
LifeSpan Biosciences
catalog: LS-C232184
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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YWHAG / 14-3-3 Gamma Antibody (aa63-92, PE) LS-C232185
LifeSpan Biosciences
catalog: LS-C232185
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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YWHAG / 14-3-3 Gamma Antibody (aa63-92, FITC) LS-C232187
LifeSpan Biosciences
catalog: LS-C232187
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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YWHAG / 14-3-3 Gamma Antibody (aa63-92, Azide-free, HRP) LS-C248984
LifeSpan Biosciences
catalog: LS-C248984
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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YWHAG / 14-3-3 Gamma Antibody (aa63-92) LS-C330323
LifeSpan Biosciences
catalog: LS-C330323
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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YWHAG / 14-3-3 Gamma Antibody LS-C332336
LifeSpan Biosciences
catalog: LS-C332336
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using YWHAG antibody.

Immunohistochemistry of paraffin-embedded Human lung cancer tissue using YWHAG antibody.
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YWHAG / 14-3-3 Gamma Antibody (Internal) LS-C353011
LifeSpan Biosciences
catalog: LS-C353011
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of 14-3-3 gamma expression in A549 (A); A431 (B); K562 (C); NIH3T3 (D); PC12 (E); rat liver (F) whole cell lysates.

Immunofluorescent analysis of 14-3-3 gamma staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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YWHAG / 14-3-3 Gamma Antibody LS-C357029
LifeSpan Biosciences
catalog: LS-C357029
domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-14-3-3 gamma Antibody at 1:1000 dilution Lane 1: HeLa whole cell lysates Lane 2: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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YWHAG / 14-3-3 Gamma Antibody (30-110 aa, Internal) LS-C381795
LifeSpan Biosciences
catalog: LS-C381795
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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