IHC-plus™ VGF Antibody LS-B2623
LifeSpan Biosciences
catalog: LS-B2623

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of VGF in human brain tissue lysate with VGF antibody at (A) 0.5 and (B) 1 ug/ml.

Anti-VGF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of VGF in Human Brain cells with VGF antibody at 20 ug/ml.
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IHC-plus™ VGF Antibody (aa454-503) LS-B5317
LifeSpan Biosciences
catalog: LS-B5317

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-VGF antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot analysis of lysates from RAW264.7 cells, using VGF Antibody. The lane on the right is blocked with the synthesized peptide.
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VGF Antibody (aa503-552) LS-C110670
LifeSpan Biosciences
catalog: LS-C110670

domestic rabbit polyclonal
reactivity: human
application: western blot

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VGF Antibody (aa330-449, FITC) LS-C302815
LifeSpan Biosciences
catalog: LS-C302815

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot

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VGF Antibody (aa582-600) LS-C313115
LifeSpan Biosciences
catalog: LS-C313115

domestic rabbit polyclonal
reactivity: human
application: western blot

WB of VGF antibody. Lane 1: Rat Brain Tissue. Lane 2: U87 Cell Lysate. Lane 3: U87 Cell Lysate. Lane 4: SHG Cell Lysate. Lane 5: NEURO Cell Lysate.
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VGF Antibody (aa330-449) LS-C314233
LifeSpan Biosciences
catalog: LS-C314233

mouse monoclonal
reactivity: human
application: western blot

Western Blot; Sample: Rat Liver lysate; Primary Ab: 1µg/ml Mouse Anti-Rat ARG Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

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IHC-plus™ VGF Antibody (aa398-447) LS-B11891
LifeSpan Biosciences
catalog: LS-B11891

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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VGF Antibody (C-Terminus) LS-C352987
LifeSpan Biosciences
catalog: LS-C352987

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of VGF expression in human brain (A); RAW264.7 (B); PC12 (C) whole cell lysates.

Immunohistochemical analysis of VGF staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of VGF staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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VGF Antibody (aa431-490) LS-C357025
LifeSpan Biosciences
catalog: LS-C357025

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-VGF Antibody at 1:1000 dilution + human brain lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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VGF Antibody (430-510 aa, C-terminal) LS-C386638
LifeSpan Biosciences
catalog: LS-C386638

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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VGF Antibody (441-490 aa, Internal) LS-C387055
LifeSpan Biosciences
catalog: LS-C387055

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of VGF antibody

Immunohistochemistry of VGF antibody
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