SUMO1 / SMT3 Antibody LS-C15472
LifeSpan Biosciences
catalog: LS-C15472

mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

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IHC-plus™ SUMO1 / SMT3 Antibody LS-B594
LifeSpan Biosciences
catalog: LS-B594

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-SUMO1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of Sumo in HL60 cells with Sumo antibody at 5 ug/ml.

Immunofluorescence of Sumo in HL60 cells with Sumo antibody at 20 ug/ml.
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SUMO1 / SMT3 Antibody (clone 66AT1273.94) LS-C96460
LifeSpan Biosciences
catalog: LS-C96460

mouse monoclonal (66AT1273.94)
reactivity: human
application: western blot
citations: 1

The SUMO1 monoclonal antibody is used in Western blot to detect SUMO1 in HL60 cell lysate.
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SUMO1 / SMT3 Antibody (aa55-86) LS-C98764
LifeSpan Biosciences
catalog: LS-C98764

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels: cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB SUMO-1 antibody. Bottom panels: cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with SUMO-2/3 antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

SUMO1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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SUMO1 / SMT3 Antibody LS-C161612
LifeSpan Biosciences
catalog: LS-C161612

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of SUMO1 Antibody in MCF-7 cell line lysates (35 ug/lane). SUMO1 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human brain tissue reacted with SUMO1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

SUMO1 Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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SUMO1 / SMT3 Antibody LS-C39039
LifeSpan Biosciences
catalog: LS-C39039

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

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SUMO1 / SMT3 Antibody (N-Terminus) LS-C47087
LifeSpan Biosciences
catalog: LS-C47087

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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SUMO1 / SMT3 Antibody LS-C75735
LifeSpan Biosciences
catalog: LS-C75735

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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SUMO1 / SMT3 Antibody (N-Terminus) LS-C94882
LifeSpan Biosciences
catalog: LS-C94882

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry

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SUMO1 / SMT3 Antibody LS-C59544
LifeSpan Biosciences
catalog: LS-C59544

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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SUMO1 / SMT3 Antibody (aa6-21) LS-C91290
LifeSpan Biosciences
catalog: LS-C91290

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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SUMO1 / SMT3 Antibody (aa6-21) LS-C91291
LifeSpan Biosciences
catalog: LS-C91291

domestic sheep polyclonal
reactivity: human
application: western blot, immunohistochemistry

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SUMO1 / SMT3 Antibody LS-C98837
LifeSpan Biosciences
catalog: LS-C98837

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunohistochemical of paraffin-embedded H.bladder section using Pan SUMO Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent confocal image of HeLa cell stained with Pan SUMO Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Pan SUMO primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). Pan SUMO immunoreactivity is localized to Nucleus significantly.

SUMO3 Antibody western blot of 293 cell line and rat liver tissue lysates (35 ug/lane). The SUMO3 antibody detected the SUMO3 protein (arrow).
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SUMO1 / SMT3 Antibody LS-C98838
LifeSpan Biosciences
catalog: LS-C98838

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The Pan SUMO Antibody is used in Western blot to detect SUMO1 in GST(26kd)-Sumo1 protein bacterial lysate.
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SUMO1 / SMT3 Antibody LS-C132036
LifeSpan Biosciences
catalog: LS-C132036

mouse monoclonal
reactivity: human
application: western blot, ELISA

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SUMO1 / SMT3 Antibody (aa2-101) LS-C132039
LifeSpan Biosciences
catalog: LS-C132039

domestic goat polyclonal
reactivity: human
application: western blot

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SUMO1 / SMT3 Antibody LS-C140646
LifeSpan Biosciences
catalog: LS-C140646

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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SUMO1 / SMT3 Antibody (N-Terminus) LS-C145684
LifeSpan Biosciences
catalog: LS-C145684

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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SUMO1 / SMT3 Antibody (clone 3D7) LS-C139325
LifeSpan Biosciences
catalog: LS-C139325

mouse monoclonal (3D7)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged SUMO1 is 0.1 ng/ml as a capture antibody.
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IHC-plus™ SUMO1 / SMT3 Antibody (aa1-50) LS-B7650
LifeSpan Biosciences
catalog: LS-B7650

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-SUMO1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of NIH/3T3 cells, using Sumo1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using Sumo1 Antibody. The lane on the right is blocked with the synthesized peptide.
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SUMO1 / SMT3 Antibody (clone 5B12) LS-C179834
LifeSpan Biosciences
catalog: LS-C179834

mouse monoclonal (5B12)
reactivity: human
application: western blot, immunocytochemistry

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SUMO1 / SMT3 Antibody (AP) LS-C270691
LifeSpan Biosciences
catalog: LS-C270691

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (APC) LS-C270692
LifeSpan Biosciences
catalog: LS-C270692

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (Biotin) LS-C270693
LifeSpan Biosciences
catalog: LS-C270693

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (Azide-free, HRP) LS-C270694
LifeSpan Biosciences
catalog: LS-C270694

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (FITC) LS-C270695
LifeSpan Biosciences
catalog: LS-C270695

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (PE) LS-C270696
LifeSpan Biosciences
catalog: LS-C270696

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (aa55-86, AP) LS-C270697
LifeSpan Biosciences
catalog: LS-C270697

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (aa55-86, APC) LS-C270698
LifeSpan Biosciences
catalog: LS-C270698

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (aa55-86, Biotin) LS-C270699
LifeSpan Biosciences
catalog: LS-C270699

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (aa55-86, FITC) LS-C270700
LifeSpan Biosciences
catalog: LS-C270700

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (aa55-86, Azide-free, HRP) LS-C270701
LifeSpan Biosciences
catalog: LS-C270701

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (aa55-86, PE) LS-C270702
LifeSpan Biosciences
catalog: LS-C270702

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody (AP) LS-C282586
LifeSpan Biosciences
catalog: LS-C282586

mouse monoclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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SUMO1 / SMT3 Antibody (APC) LS-C282587
LifeSpan Biosciences
catalog: LS-C282587

mouse monoclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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SUMO1 / SMT3 Antibody (Biotin) LS-C282588
LifeSpan Biosciences
catalog: LS-C282588

mouse monoclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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SUMO1 / SMT3 Antibody (Azide-free, HRP) LS-C282589
LifeSpan Biosciences
catalog: LS-C282589

mouse monoclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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SUMO1 / SMT3 Antibody (FITC) LS-C282590
LifeSpan Biosciences
catalog: LS-C282590

mouse monoclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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SUMO1 / SMT3 Antibody (PE) LS-C282597
LifeSpan Biosciences
catalog: LS-C282597

mouse monoclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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SUMO1 / SMT3 Antibody (aa15-30) LS-C313322
LifeSpan Biosciences
catalog: LS-C313322

domestic rabbit polyclonal
reactivity: human
application: western blot

WB of SUMO1 / SMT3 antibody. Lane 1: Rat Spleen Tissue Lysate. Lane 2: Human Placenta Tissue Lysate.
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SUMO1 / SMT3 Antibody LS-C192924
LifeSpan Biosciences
catalog: LS-C192924

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of A431 cell line, using SUMO1 antibody.
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SUMO1 / SMT3 Antibody (aa1-30) LS-C325498
LifeSpan Biosciences
catalog: LS-C325498

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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SUMO1 / SMT3 Antibody (aa55-86) LS-C325501
LifeSpan Biosciences
catalog: LS-C325501

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody LS-C330191
LifeSpan Biosciences
catalog: LS-C330191

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SUMO1 / SMT3 Antibody LS-C331925
LifeSpan Biosciences
catalog: LS-C331925

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of HeLa cells.

Immunohistochemistry of paraffin-embedded rat testis.

Immunofluorescence analysis of GFP-RNF168 trangenic U2OS cells.
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SUMO1 / SMT3 Antibody (C-Terminus) LS-C343812
LifeSpan Biosciences
catalog: LS-C343812

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot: SUMOylation assay utilising SUMO-1, SUMO E1 (BML-UW9330), SUMO E2 (BML-UW9320) and RANGAP1 as substrate in presence (lane 2) and absence (lane 1) of ATP after SDS-PAGE and blotting to PVDF with subsequent probing with BML-PW9460.
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SUMO1 / SMT3 Antibody (N-Terminus) LS-C352975
LifeSpan Biosciences
catalog: LS-C352975

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of SUMO1 expression in MCF7 (A); A431 (B); HeLa (C); mouse kidney (D); rat kidney (E) whole cell lysates.

Immunohistochemical analysis of SUMO1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of SUMO1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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SUMO1 / SMT3 Antibody (1-80 aa, N-terminal) LS-C386244
LifeSpan Biosciences
catalog: LS-C386244

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of SUMO-1 antibody
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