HR6B / UBE2B Antibody (aa117-146) LS-C98024
LifeSpan Biosciences
catalog: LS-C98024
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot of UBE2B (arrow) using rabbit polyclonal UBE2B Antibody (E132). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2B gene.
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HR6B / UBE2B Antibody LS-C109312
LifeSpan Biosciences
catalog: LS-C109312
domestic rabbit polyclonal
reactivity: human
application: western blot

Sample (30 ug of whole cell lysate). A: Hela. 12% SDS PAGE. UBE2B antibody. UBE2B antibody diluted at 1:1000.
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HR6B / UBE2B Antibody (clone 4C3) LS-C198393
LifeSpan Biosciences
catalog: LS-C198393
mouse monoclonal (4C3)
reactivity: human
application: western blot, ELISA

Western Blot analysis of UBE2B expression in transfected 293T cell line by UBE2B monoclonal antibody (M06), clone 4C3.Lane 1: UBE2B transfected lysate(17.3 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged UBE2B is approximately 3 ng/ml as a capture antibody.
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HR6B / UBE2B Antibody (aa117-146, Biotin) LS-C283916
LifeSpan Biosciences
catalog: LS-C283916
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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HR6B / UBE2B Antibody (aa117-146, APC) LS-C283917
LifeSpan Biosciences
catalog: LS-C283917
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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HR6B / UBE2B Antibody (aa117-146, FITC) LS-C283918
LifeSpan Biosciences
catalog: LS-C283918
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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HR6B / UBE2B Antibody (aa117-146, Azide-free, HRP) LS-C283919
LifeSpan Biosciences
catalog: LS-C283919
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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HR6B / UBE2B Antibody (aa117-146, PE) LS-C283920
LifeSpan Biosciences
catalog: LS-C283920
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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HR6B / UBE2B Antibody (aa117-146) LS-C328989
LifeSpan Biosciences
catalog: LS-C328989
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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HR6B / UBE2B Antibody LS-C334634
LifeSpan Biosciences
catalog: LS-C334634
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded rat lung tissue.
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HR6B / UBE2B Antibody (clone OTI1E11) LS-C338792
LifeSpan Biosciences
catalog: LS-C338792
mouse monoclonal (OTI1E11)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2B.
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