IHC-plus™ CLDN5 / Claudin 5 Antibody LS-B2331
LifeSpan Biosciences
catalog: LS-B2331

mouse monoclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-CLDN5 / Claudin 5 antibody IHC of rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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PathPlus™ CLDN5 / Claudin 5 Antibody (C-Terminus) LS-B3064
LifeSpan Biosciences
catalog: LS-B3064

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Anti-CLDN5 / Claudin 5 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.

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IHC-plus™ CLDN5 / Claudin 5 Antibody (aa171-220) LS-B11331
LifeSpan Biosciences
catalog: LS-B11331

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Anti-CLDN5 / Claudin 5 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot of Claudin 5 (S201) pAb at a 1:500 dilution. Lane 1: HeLa cell lysate treated with LPS. Lane 2: Mouse liver tissue lysate. Lane 3: Rat lung tissue lysate.
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PathPlus™ CLDN5 / Claudin 5 Antibody (aa169-218) LS-B5971
LifeSpan Biosciences
catalog: LS-B5971

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

Anti-CLDN5 / Claudin 5 antibody IHC of human uterus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot analysis of lysates from A549 cells, using Claudin 5 Antibody. The lane on the right is blocked with the synthesized peptide.
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CLDN5 / Claudin 5 Antibody (C-Terminus) LS-C6157
LifeSpan Biosciences
catalog: LS-C6157

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

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CLDN5 / Claudin 5 Antibody (C-Terminus) LS-C80067
LifeSpan Biosciences
catalog: LS-C80067

domestic rabbit polyclonal
reactivity: human
application: western blot

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CLDN5 / Claudin 5 Antibody (clone 3D8) LS-C198352
LifeSpan Biosciences
catalog: LS-C198352

mouse monoclonal (3D8)
reactivity: human
application: ELISA

Detection limit for recombinant GST tagged CLDN5 is approximately 0.1 ng/ml as a capture antibody.
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CLDN5 / Claudin 5 Antibody (phospho-Tyr217) LS-C198811
LifeSpan Biosciences
catalog: LS-C198811

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain, using Claudin 5 (Phospho-Tyr217) Antibody. The picture on the right is blocked with the phospho peptide.
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CLDN5 / Claudin 5 Antibody (aa169-218) LS-C291250
LifeSpan Biosciences
catalog: LS-C291250

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

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CLDN5 / Claudin 5 Antibody (C-Terminus) LS-C352946
LifeSpan Biosciences
catalog: LS-C352946

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Claudin 5 expression in HeLa (A); mouse liver (B); rat lung (C) whole cell lysates.

Immunohistochemical analysis of Claudin 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Claudin 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CLDN5 / Claudin 5 Antibody (clone 4C3C2, AF488) LS-C354907
LifeSpan Biosciences
catalog: LS-C354907

mouse monoclonal (4C3C2)
reactivity: human, mouse
conjugate: AF488
application: western blot, ELISA, immunocytochemistry, immunoprecipitation

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CLDN5 / Claudin 5 Antibody (aa191-218) LS-C357455
LifeSpan Biosciences
catalog: LS-C357455

domestic rabbit polyclonal
reactivity: human
application: western blot

Claudin 5 antibody Western blot. All lanes: Anti Claudin-5 at 0.5 ug/ml. WB: Rat Liver Tissue Lysate at 50 ug. Predicted band size: 23 kD. Observed band size: 23 kD.
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CLDN5 / Claudin 5 Antibody (150-230 aa) LS-C381676
LifeSpan Biosciences
catalog: LS-C381676

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry

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CLDN5 / Claudin 5 Antibody (150-230 aa) LS-C382739
LifeSpan Biosciences
catalog: LS-C382739

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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CLDN5 / Claudin 5 Antibody (140-220 aa, C-terminal) LS-C382740
LifeSpan Biosciences
catalog: LS-C382740

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Claudin-5 antibody
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