BUB1B / BubR1 Antibody (aa1-350, clone 8G1) LS-C18942
LifeSpan Biosciences
catalog: LS-C18942

mouse monoclonal (8G1)
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation

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IHC-plus™ BUB1B / BubR1 Antibody (clone 2G5) LS-B4289
LifeSpan Biosciences
catalog: LS-B4289

mouse monoclonal (2G5)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, proximity ligation assay

Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.

Western blot of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody clone 2G5. GAPDH ( 36.1 kD ) used as specificity an.

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.
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BUB1B / BubR1 Antibody (aa1-350, clone 8G1) LS-C2771
LifeSpan Biosciences
catalog: LS-C2771

mouse monoclonal (8G1)
reactivity: human
application: western blot, immunocytochemistry
citations: 3

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BUB1B / BubR1 Antibody LS-C26648
LifeSpan Biosciences
catalog: LS-C26648

domestic sheep polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry

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BUB1B / BubR1 Antibody LS-C26649
LifeSpan Biosciences
catalog: LS-C26649

domestic sheep polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry

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BUB1B / BubR1 Antibody LS-C26650
LifeSpan Biosciences
catalog: LS-C26650

domestic sheep polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry

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BUB1B / BubR1 Antibody (N-Terminus) LS-C77252
LifeSpan Biosciences
catalog: LS-C77252

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry

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BUB1B / BubR1 Antibody (aa3-33) LS-C101149
LifeSpan Biosciences
catalog: LS-C101149

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of BUB1B (arrow) using rabbit polyclonal BUB1B Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BUB1B gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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BUB1B / BubR1 Antibody (clone 2G9) LS-C104868
LifeSpan Biosciences
catalog: LS-C104868

mouse monoclonal (2G9)
reactivity: human
application: western blot, ELISA, immunocytochemistry

BUB1B monoclonal antibody (M01), clone 2G9. Western blot of BUB1B expression in human spleen.

Immunofluorescence of monoclonal antibody to BUB1B on HeLa cell (antibody concentration 10 ug/ml).
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IHC-plus™ BUB1B / BubR1 Antibody (clone 3F2) LS-B4291
LifeSpan Biosciences
catalog: LS-B4291

mouse monoclonal (3F2)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

BUB1B monoclonal antibody, clone 3F2 Western blot of BUB1B expression in HeLa NE.

Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.

Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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BUB1B / BubR1 Antibody (aa341-390) LS-C118698
LifeSpan Biosciences
catalog: LS-C118698

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HeLa and HepG2 cells, treated with H2O2 100uM 30', using BUB1B Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ BUB1B / BubR1 Antibody LS-B4854
LifeSpan Biosciences
catalog: LS-B4854

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: A431. A: H1299. 7.5% SDS PAGE. BUB1B antibody diluted at 1:1000.

Anti-BUB1B / BubR1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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BUB1B / BubR1 Antibody LS-C125211
LifeSpan Biosciences
catalog: LS-C125211

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry

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BUB1B / BubR1 Antibody (clone P10.8G1.G11.H12) LS-C137474
LifeSpan Biosciences
catalog: LS-C137474

mouse monoclonal (P10.8G1.G11.H12)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

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BUB1B / BubR1 Antibody (N-Terminus, clone 8G1, Biotin) LS-C141113
LifeSpan Biosciences
catalog: LS-C141113

mouse monoclonal (8G1)
reactivity: human
conjugate: biotin
application: western blot, immunocytochemistry

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BUB1B / BubR1 Antibody (N-Terminus, clone 8G1, DY650) LS-C141114
LifeSpan Biosciences
catalog: LS-C141114

mouse monoclonal (8G1)
reactivity: human
application: western blot, immunocytochemistry

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BUB1B / BubR1 Antibody (N-Terminus, clone 8G1, DY488) LS-C141115
LifeSpan Biosciences
catalog: LS-C141115

mouse monoclonal (8G1)
reactivity: human
application: western blot, immunocytochemistry

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BUB1B / BubR1 Antibody (N-Terminus, clone 8G1, DY550) LS-C141117
LifeSpan Biosciences
catalog: LS-C141117

mouse monoclonal (8G1)
reactivity: human
application: western blot, immunocytochemistry

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BUB1B / BubR1 Antibody (N-Terminus) LS-C144900
LifeSpan Biosciences
catalog: LS-C144900

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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BUB1B / BubR1 Antibody (clone OTI5D9) LS-C114630
LifeSpan Biosciences
catalog: LS-C114630

mouse monoclonal (OTI5D9)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of extracts (35 ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.
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BUB1B / BubR1 Antibody (clone OTI3C7) LS-C114631
LifeSpan Biosciences
catalog: LS-C114631

mouse monoclonal (OTI3C7)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.
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BUB1B / BubR1 Antibody (clone OTI11G2) LS-C114637
LifeSpan Biosciences
catalog: LS-C114637

mouse monoclonal (OTI11G2)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of extracts (35 ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.
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BUB1B / BubR1 Antibody (clone OTI11D7) LS-C114638
LifeSpan Biosciences
catalog: LS-C114638

mouse monoclonal (OTI11D7)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of extracts (35 ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.
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BUB1B / BubR1 Antibody (clone OTI15E11) LS-C114683
LifeSpan Biosciences
catalog: LS-C114683

mouse monoclonal (OTI15E11)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.
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BUB1B / BubR1 Antibody (clone OTI10E7) LS-C114703
LifeSpan Biosciences
catalog: LS-C114703

mouse monoclonal (OTI10E7)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of extracts (35 ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

IHC of paraffin-embedded breast tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.
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BUB1B / BubR1 Antibody (clone OTI6E5) LS-C114704
LifeSpan Biosciences
catalog: LS-C114704

mouse monoclonal (OTI6E5)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.
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BUB1B / BubR1 Antibody (clone OTI3D9) LS-C114705
LifeSpan Biosciences
catalog: LS-C114705

mouse monoclonal (OTI3D9)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of extracts (35 ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

IHC of paraffin-embedded colon tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescent staining of HeLa cells using anti-BUB1B mouse monoclonal antibody.
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BUB1B / BubR1 Antibody (clone OTI6E3) LS-C114772
LifeSpan Biosciences
catalog: LS-C114772

mouse monoclonal (OTI6E3)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Western blot of extracts (35 ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BUB1B antibody, and then analyzed by flow cytometry.
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BUB1B / BubR1 Antibody (Thr368) LS-C176888
LifeSpan Biosciences
catalog: LS-C176888

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of ubR1 (T368) pAb in extracts from HeLa cells treated with H2O2 100uM 30'.
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BUB1B / BubR1 Antibody (clone 8G1) LS-C179698
LifeSpan Biosciences
catalog: LS-C179698

mouse monoclonal (8G1)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

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BUB1B / BubR1 Antibody (clone 2097C2a) LS-C184699
LifeSpan Biosciences
catalog: LS-C184699

mouse monoclonal (2097C2a)
reactivity: human
application: western blot, immunocytochemistry

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BUB1B / BubR1 Antibody (clone 11C1018) LS-C183765
LifeSpan Biosciences
catalog: LS-C183765

domestic rabbit monoclonal (11C1018)
reactivity: human
application: western blot

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BUB1B / BubR1 Antibody (aa3-33, APC) LS-C275683
LifeSpan Biosciences
catalog: LS-C275683

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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BUB1B / BubR1 Antibody (aa3-33, AP) LS-C275684
LifeSpan Biosciences
catalog: LS-C275684

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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BUB1B / BubR1 Antibody (aa3-33, Biotin) LS-C275685
LifeSpan Biosciences
catalog: LS-C275685

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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BUB1B / BubR1 Antibody (aa3-33, FITC) LS-C275686
LifeSpan Biosciences
catalog: LS-C275686

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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BUB1B / BubR1 Antibody (aa3-33, Azide-free, HRP) LS-C275688
LifeSpan Biosciences
catalog: LS-C275688

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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BUB1B / BubR1 Antibody (aa3-33, PE) LS-C275689
LifeSpan Biosciences
catalog: LS-C275689

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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BUB1B / BubR1 Antibody (aa1-50) LS-C286717
LifeSpan Biosciences
catalog: LS-C286717

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

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BUB1B / BubR1 Antibody (aa350-400) LS-C287279
LifeSpan Biosciences
catalog: LS-C287279

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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BUB1B / BubR1 Antibody (aa1-50) LS-C287828
LifeSpan Biosciences
catalog: LS-C287828

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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BUB1B / BubR1 Antibody (aa3-33) LS-C330359
LifeSpan Biosciences
catalog: LS-C330359

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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BUB1B / BubR1 Antibody LS-C331686
LifeSpan Biosciences
catalog: LS-C331686

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using BUB1B antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunohistochemistry of paraffin-embedded human breast cancer using BUB1B antibodyat dilution of 1:200 (40x lens).
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BUB1B / BubR1 Antibody (310-390 aa, Internal) LS-C382344
LifeSpan Biosciences
catalog: LS-C382344

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of BubR1 antibody
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