TDG / Thymine DNA Glycosylase Antibody (aa81-110) LS-C99357
LifeSpan Biosciences
catalog: LS-C99357
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Western blot of TDG antibody in mouse lung tissue lysates (35 ug/lane). TDG (arrow) was detected using the purified antibody.

TDG Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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TDG / Thymine DNA Glycosylase Antibody (aa281-310) LS-C99358
LifeSpan Biosciences
catalog: LS-C99358
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Western blot of TDG antibody in K562 cell line lysates (35 ug/lane). TDG (arrow) was detected using the purified antibody.

Flow cytometric of K562 cells using TDG Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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TDG / Thymine DNA Glycosylase Antibody (aa31-80) LS-C119026
LifeSpan Biosciences
catalog: LS-C119026
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from Jurkat cells, using TDG Antibody. The lane on the right is blocked with the synthesized peptide.
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TDG / Thymine DNA Glycosylase Antibody (Lys90) LS-C176808
LifeSpan Biosciences
catalog: LS-C176808
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of TDG (K90) pAb in extracts from Jurkat cells.
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TDG / Thymine DNA Glycosylase Antibody (clone AT2F7) LS-C200834
LifeSpan Biosciences
catalog: LS-C200834
mouse monoclonal (AT2F7)
reactivity: human
application: western blot, ELISA

Western Blot: The cell lysates of HeLa (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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TDG / Thymine DNA Glycosylase Antibody (aa360-410) LS-C291004
LifeSpan Biosciences
catalog: LS-C291004
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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TDG / Thymine DNA Glycosylase Antibody LS-C305941
LifeSpan Biosciences
catalog: LS-C305941
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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TDG / Thymine DNA Glycosylase Antibody LS-C334266
LifeSpan Biosciences
catalog: LS-C334266
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of A549 cells.
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TDG / Thymine DNA Glycosylase Antibody LS-C335140
LifeSpan Biosciences
catalog: LS-C335140
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of mouse brain, using TDG antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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TDG / Thymine DNA Glycosylase Antibody (Internal) LS-C354097
LifeSpan Biosciences
catalog: LS-C354097
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of Thymine DNA Glycosylase expression in HeLa (A); mouse lung (B); rat lung (C) whole cell lysates.

Immunofluorescent analysis of Thymine DNA Glycosylase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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TDG / Thymine DNA Glycosylase Antibody (aa141-410) LS-C377568
LifeSpan Biosciences
catalog: LS-C377568
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot. All lanes: G/T mismatch-specific thymine DNA glycosylase antibody at 8 ug/ml+mouse brain tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 46 kDa. Observed band size: 46 kDa.
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TDG / Thymine DNA Glycosylase Antibody (aa141-410) LS-C377570
LifeSpan Biosciences
catalog: LS-C377570
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: G/T mismatch-specific thymine DNA glycosylase antibody at 6 ug/ml+mouse brain tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 46 kDa. Observed band size: 46 kDa Immunohistochemistry.

Immunohistochemistry of paraffin-embedded human heart using antibody at 1:100 dilution.
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TDG / Thymine DNA Glycosylase Antibody (1-80 aa, Internal) LS-C386361
LifeSpan Biosciences
catalog: LS-C386361
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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