LifeSpan Biosciences BTK antibody

IHC-plus™ BTK Antibody LS-B4855
LifeSpan Biosciences
catalog: LS-B4855

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: Raji. 7.5% SDS PAGE. BTK antibody diluted at 1:5000.

Anti-BTK antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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BTK Antibody (aa188-237) LS-C117550
LifeSpan Biosciences
catalog: LS-C117550

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BTK Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, using BTK Antibody. The lane on the right is blocked with the synthesized peptide.
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BTK Antibody LS-C455
LifeSpan Biosciences
catalog: LS-C455

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of BTK in U937 cell lysate with BTK antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunocytochemistry of BTK in Daudi cells with BTK antibody at 10 ug/ml.

Immunofluorescence of BTK in Daudi cells with BTK antibody at 10 ug/ml.
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BTK Antibody (phospho-Ser180) LS-C4243
LifeSpan Biosciences
catalog: LS-C4243

mouse monoclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunoprecipitation

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BTK Antibody (clone 7F12H4) LS-C47221
LifeSpan Biosciences
catalog: LS-C47221

mouse monoclonal (7F12H4)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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BTK Antibody (aa209-239) LS-C99168
LifeSpan Biosciences
catalog: LS-C99168

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

The anti-BTK antibody is used in Western blot to detect BTK in Ramos cell lysate.

Confocal immunofluorescence of BTK Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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BTK Antibody (aa27-55) LS-C156886
LifeSpan Biosciences
catalog: LS-C156886

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of BTK (arrow) using rabbit polyclonal BTK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BTK gene.

BTK Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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BTK Antibody (aa209-239) LS-C159189
LifeSpan Biosciences
catalog: LS-C159189

mouse monoclonal
reactivity: human
application: western blot

BTK Antibody western blot of Ramos cell line lysates (35 ug/lane). The BTK antibody detected the BTK protein (arrow).
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BTK Antibody (clone OTI12D4) LS-C115131
LifeSpan Biosciences
catalog: LS-C115131

mouse monoclonal (OTI12D4)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.

IHC of paraffin-embedded Kidney tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
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BTK Antibody (clone OTI12H4) LS-C115132
LifeSpan Biosciences
catalog: LS-C115132

mouse monoclonal (OTI12H4)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

IHC of paraffin-embedded liver tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
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BTK Antibody (clone OTI6H9) LS-C115134
LifeSpan Biosciences
catalog: LS-C115134

mouse monoclonal (OTI6H9)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
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BTK Antibody (clone OTI3A2) LS-C115135
LifeSpan Biosciences
catalog: LS-C115135

mouse monoclonal (OTI3A2)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
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BTK Antibody (clone OTI3E9) LS-C115140
LifeSpan Biosciences
catalog: LS-C115140

mouse monoclonal (OTI3E9)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Kidney tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
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BTK Antibody (clone OTI1H7) LS-C115141
LifeSpan Biosciences
catalog: LS-C115141

mouse monoclonal (OTI1H7)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Kidney tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
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BTK Antibody (clone OTI13E5) LS-C115142
LifeSpan Biosciences
catalog: LS-C115142

mouse monoclonal (OTI13E5)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.

HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.
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BTK Antibody (clone OTI10E10) LS-C115157
LifeSpan Biosciences
catalog: LS-C115157

mouse monoclonal (OTI10E10)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Human liver tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
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BTK Antibody (clone OTI15G8) LS-C115158
LifeSpan Biosciences
catalog: LS-C115158

mouse monoclonal (OTI15G8)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

IHC of paraffin-embedded colon tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
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BTK Antibody (phospho-Tyr223) LS-C180161
LifeSpan Biosciences
catalog: LS-C180161

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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BTK Antibody (phospho-Tyr223, Biotin) LS-C180846
LifeSpan Biosciences
catalog: LS-C180846

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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BTK Antibody (phospho-Tyr223, DY650) LS-C180847
LifeSpan Biosciences
catalog: LS-C180847

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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BTK Antibody (phospho-Tyr223, DY488) LS-C180848
LifeSpan Biosciences
catalog: LS-C180848

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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BTK Antibody (phospho-Tyr223, DY550) LS-C180850
LifeSpan Biosciences
catalog: LS-C180850

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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BTK Antibody (phospho-Tyr551) LS-C192176
LifeSpan Biosciences
catalog: LS-C192176

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot of p-Btk (Y551) pAb in extracts from HeLa cells.
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BTK Antibody (phospho-Tyr223) LS-C198702
LifeSpan Biosciences
catalog: LS-C198702

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from HeLa cells treated with Serum 10% 15', using BTK (Phospho-Tyr223) Antibody. The lane on the right is blocked with the phospho peptide.
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BTK Antibody (phospho-Tyr551) LS-C198703
LifeSpan Biosciences
catalog: LS-C198703

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from HeLa cells treated with H2O2 100uM 30', using BTK (Phospho-Tyr551) Antibody. The lane on the right is blocked with the phospho peptide.
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BTK Antibody (aa27-55, AP) LS-C252769
LifeSpan Biosciences
catalog: LS-C252769

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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BTK Antibody (aa27-55, APC) LS-C252770
LifeSpan Biosciences
catalog: LS-C252770

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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BTK Antibody (aa27-55, Biotin) LS-C252771
LifeSpan Biosciences
catalog: LS-C252771

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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BTK Antibody (aa27-55, FITC) LS-C252772
LifeSpan Biosciences
catalog: LS-C252772

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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BTK Antibody (aa27-55, Azide-free, HRP) LS-C252773
LifeSpan Biosciences
catalog: LS-C252773

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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BTK Antibody (aa27-55, PE) LS-C252777
LifeSpan Biosciences
catalog: LS-C252777

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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BTK Antibody (aa209-239, AP) LS-C275701
LifeSpan Biosciences
catalog: LS-C275701

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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BTK Antibody (aa209-239, FITC) LS-C275702
LifeSpan Biosciences
catalog: LS-C275702

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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BTK Antibody (aa209-239, Biotin) LS-C275703
LifeSpan Biosciences
catalog: LS-C275703

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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BTK Antibody (aa209-239, APC) LS-C275704
LifeSpan Biosciences
catalog: LS-C275704

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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BTK Antibody (aa209-239, Azide-free, HRP) LS-C275707
LifeSpan Biosciences
catalog: LS-C275707

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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BTK Antibody (aa209-239, PE) LS-C275709
LifeSpan Biosciences
catalog: LS-C275709

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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BTK Antibody LS-C192707
LifeSpan Biosciences
catalog: LS-C192707

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of Daudi cell lines, using BTK antibody.
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BTK Antibody (aa27-55) LS-C319913
LifeSpan Biosciences
catalog: LS-C319913

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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BTK Antibody LS-C331556
LifeSpan Biosciences
catalog: LS-C331556

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) mouse spleen using BTK antibody at dilution of 1:100 (40x magnification).

Immunofluorescence analysis of HeLa cells using BTK antibody. Blue: DAPI for nuclear staining.
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BTK Antibody (phospho-Tyr223) LS-C335791
LifeSpan Biosciences
catalog: LS-C335791

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of U2OS cells.
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BTK Antibody (aa396-430) LS-C344298
LifeSpan Biosciences
catalog: LS-C344298

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysates from Raji, Ramos, K562, Daudi cell line and mouse spleen tissue lysate (from left to right) with BTK Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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BTK Antibody (aa396-430) LS-C344557
LifeSpan Biosciences
catalog: LS-C344557

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysate from Ramos cell line with BTK Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
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BTK Antibody (phospho-Tyr223) LS-C353912
LifeSpan Biosciences
catalog: LS-C353912

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of BTK (pY223) expression in DLD (A); mouse liver (B); rat liver (C); rat kidney (D) whole cell lysates.
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BTK Antibody (phospho-Tyr551) LS-C353914
LifeSpan Biosciences
catalog: LS-C353914

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of BTK (pY551) expression in Jurkat (A); K562 (B) whole cell lysates.

Immunofluorescent analysis of BTK (pY551) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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BTK Antibody (phospho-Tyr223) LS-C357122
LifeSpan Biosciences
catalog: LS-C357122

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot of extracts from HeLa cells treated with serum (10%, 15mins), using BTK (phospho-Tyr223) antibody.
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BTK Antibody (C-Terminus, clone 7F12H4) LS-C359247
LifeSpan Biosciences
catalog: LS-C359247

mouse monoclonal (7F12H4)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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BTK Antibody (aa1-168) LS-C372840
LifeSpan Biosciences
catalog: LS-C372840

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot of BTK antibody.

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BTK Antibody (490-570 aa) LS-C380885
LifeSpan Biosciences
catalog: LS-C380885

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

Western blot of Phospho-Btk (Y551) antibody
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BTK Antibody (160-240 aa) LS-C380887
LifeSpan Biosciences
catalog: LS-C380887

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

Western blot of Phospho-Btk (Y223) antibody
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BTK Antibody (160-240 aa) LS-C382342
LifeSpan Biosciences
catalog: LS-C382342

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Btk antibody
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BTK Antibody (490-570 aa) LS-C387104
LifeSpan Biosciences
catalog: LS-C387104

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of Btk antibody
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