LifeSpan Biosciences TBP antibody

TBP / GTF2D Antibody (aa214-263) LS-C30808
LifeSpan Biosciences
catalog: LS-C30808

domestic rabbit polyclonal
reactivity: human
application: western blot

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TBP / GTF2D Antibody LS-C39506
LifeSpan Biosciences
catalog: LS-C39506

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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TBP / GTF2D Antibody LS-C76218
LifeSpan Biosciences
catalog: LS-C76218

mouse monoclonal
reactivity: human
application: western blot

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TBP / GTF2D Antibody (N-Terminus, clone 1TBP18) LS-C83594
LifeSpan Biosciences
catalog: LS-C83594

mouse monoclonal (1TBP18)
reactivity: human
application: western blot, immunoprecipitation

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TBP / GTF2D Antibody (aa151-200) LS-C118815
LifeSpan Biosciences
catalog: LS-C118815

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from 293 cells, using TBP Antibody. The lane on the right is blocked with the synthesized peptide.
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TBP / GTF2D Antibody (aa2-12) LS-C154976
LifeSpan Biosciences
catalog: LS-C154976

domestic goat polyclonal
reactivity: human
application: western blot

TBP antibody (0.3 ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35 ug protein in commercial extraction buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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TBP / GTF2D Antibody (aa39-50) LS-C154977
LifeSpan Biosciences
catalog: LS-C154977

domestic goat polyclonal
reactivity: human
application: western blot

TBP antibody (1 ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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TBP / GTF2D Antibody (aa301-330) LS-C161748
LifeSpan Biosciences
catalog: LS-C161748

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of TBP antibody in HL60 cell line lysates (35 ug/lane). TBP (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human testis tissue reacted with TBP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescence of TBP Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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TBP / GTF2D Antibody (clone TBPB8A9) LS-C184316
LifeSpan Biosciences
catalog: LS-C184316

mouse monoclonal (TBPB8A9)
reactivity: human
application: western blot, immunocytochemistry

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TBP / GTF2D Antibody (aa210-239) LS-C98417
LifeSpan Biosciences
catalog: LS-C98417

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

TBP Antibody western blot of K562,U251 cell line lysates (35 ug/lane). The TBP antibody detected the TBP protein (arrow).

Fluorescent confocal image of U251 cell stained with TBP Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBP primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBP immunoreactivity is localized to Nucleus significantly.

TBP / GTF2D Antibody (clone 830CT4.3.3) LS-C203916
LifeSpan Biosciences
catalog: LS-C203916

mouse monoclonal (830CT4.3.3)
reactivity: human
application: western blot

TBP Antibody western blot of HeLa,HepG2,mouse NIH/3T3 cell line lysates (35 ug/lane). The TBP antibody detected the TBP protein (arrow).
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TBP / GTF2D Antibody (aa1-50) LS-C288042
LifeSpan Biosciences
catalog: LS-C288042

domestic rabbit polyclonal
reactivity: human
application: western blot

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TBP / GTF2D Antibody (aa8-316) LS-C296575
LifeSpan Biosciences
catalog: LS-C296575

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant TBP.

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TBP / GTF2D Antibody (aa227-246) LS-C312691
LifeSpan Biosciences
catalog: LS-C312691

domestic rabbit polyclonal
reactivity: human
application: western blot

WB of TBP antibody. All lanes: Anti-TBP at 0.5ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40ug. Lane 2: MM231 Whole Cell Lysate at 40ug. Lane 3: MM231 Whole Cell Lysate at 40ug. Predicted bind size: 38KD. Observed bind size: 38KD.
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TBP / GTF2D Antibody LS-C193099
LifeSpan Biosciences
catalog: LS-C193099

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot.
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TBP / GTF2D Antibody LS-C331458
LifeSpan Biosciences
catalog: LS-C331458

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot analysis of extracts of COLO320 cells, using TBP antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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TBP / GTF2D Antibody LS-C331971
LifeSpan Biosciences
catalog: LS-C331971

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of Jurkat cell lines.
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TBP / GTF2D Antibody (Internal) LS-C358352
LifeSpan Biosciences
catalog: LS-C358352

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of TBP expression in HEK293T (A); NIH3T3 (B) whole cell lysates.

Immunohistochemical analysis of TBP staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of TBP staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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TBP / GTF2D Antibody (120-200 aa, Internal) LS-C386381
LifeSpan Biosciences
catalog: LS-C386381

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of TFIID antibody
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TBP / GTF2D Antibody LS-C387862
LifeSpan Biosciences
catalog: LS-C387862

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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