SYT1 / Synaptotagmin Antibody LS-C26317
LifeSpan Biosciences
catalog: LS-C26317
mouse monoclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
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SYT1 / Synaptotagmin Antibody LS-C39394
LifeSpan Biosciences
catalog: LS-C39394
mouse monoclonal
reactivity: human, rat
application: western blot, immunohistochemistry
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IHC-plus™ SYT1 / Synaptotagmin Antibody (clone 1A6) LS-B5543
LifeSpan Biosciences
catalog: LS-B5543
mouse monoclonal (1A6)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Detection limit for recombinant GST tagged SYT1 is approximately 1 ng/ml as a capture antibody.

Anti-SYT1 / Synaptotagmin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Synaptotagmin Antibody (aa176-225) LS-C117542
LifeSpan Biosciences
catalog: LS-C117542
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Synaptotagmin Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, using Synaptotagmin Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ SYT1 / Synaptotagmin Antibody (aa261-273) LS-B5899
LifeSpan Biosciences
catalog: LS-B5899
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

SYT1 antibody (1 ug/ml) staining of human hippocampus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-SYT1 / Synaptotagmin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
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SYT1 / Synaptotagmin Antibody (phospho-Thr202) LS-C234
LifeSpan Biosciences
catalog: LS-C234
domestic rabbit polyclonal
reactivity: human, rat
modification: phosphorylated
application: western blot
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SYT1 / Synaptotagmin Antibody (aa1-23) LS-C26314
LifeSpan Biosciences
catalog: LS-C26314
domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry, immunoprecipitation
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SYT1 / Synaptotagmin Antibody LS-C39393
LifeSpan Biosciences
catalog: LS-C39393
mouse monoclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
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SYT1 / Synaptotagmin Antibody LS-C61837
LifeSpan Biosciences
catalog: LS-C61837
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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SYT1 / Synaptotagmin Antibody (aa10-60) LS-C94909
LifeSpan Biosciences
catalog: LS-C94909
domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry
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SYT1 / Synaptotagmin Antibody (clone 8G11B10) LS-C109115
LifeSpan Biosciences
catalog: LS-C109115
mouse monoclonal (8G11B10)
reactivity: human
application: western blot, ELISA
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SYT1 / Synaptotagmin Antibody LS-C124629
LifeSpan Biosciences
catalog: LS-C124629
chicken polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
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SYT1 / Synaptotagmin Antibody LS-C132335
LifeSpan Biosciences
catalog: LS-C132335
domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry
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SYT1 / Synaptotagmin Antibody (aa231-280) LS-C135523
LifeSpan Biosciences
catalog: LS-C135523
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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SYT1 / Synaptotagmin Antibody LS-C149139
LifeSpan Biosciences
catalog: LS-C149139
chicken polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
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SYT1 / Synaptotagmin Antibody (aa349-377) LS-C161650
LifeSpan Biosciences
catalog: LS-C161650
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

SYT1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The SYT1 antibody detected the SYT1 protein (arrow).

SYT1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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SYT1 / Synaptotagmin Antibody (aa104-153) LS-C170312
LifeSpan Biosciences
catalog: LS-C170312
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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SYT1 / Synaptotagmin Antibody (Thr196) LS-C176229
LifeSpan Biosciences
catalog: LS-C176229
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of Synaptotagmin (T196) pAb in extracts from HeLa cells.

IHC of Synaptotagmin (T196) pAb in paraffin-embedded human breast carcinoma tissue.
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SYT1 / Synaptotagmin Antibody (Met303) LS-C176230
LifeSpan Biosciences
catalog: LS-C176230
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of Synaptotagmin (M303) pAb in extracts from 293 cells Forskolin 40nM 30' or 293 cells Sorbitol 0.4M 30'.

IHC of Synaptotagmin (M303) pAb in paraffin-embedded human breast carcinoma tissue.
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SYT1 / Synaptotagmin Antibody (clone 3F10) LS-C179415
LifeSpan Biosciences
catalog: LS-C179415
mouse monoclonal (3F10)
reactivity: human, rat
application: western blot
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SYT1 / Synaptotagmin Antibody LS-C195147
LifeSpan Biosciences
catalog: LS-C195147
chicken polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry
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SYT1 / Synaptotagmin Antibody (phospho-Ser309) LS-C199627
LifeSpan Biosciences
catalog: LS-C199627
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Synaptotagmin (Phospho-Ser309) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with Sobital 0.4M 30', using Synaptotagmin (Phospho-Ser309) Antibody. The lane on the right is blocked with the phospho peptide.
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SYT1 / Synaptotagmin Antibody (phospho-Thr202) LS-C199628
LifeSpan Biosciences
catalog: LS-C199628
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Synaptotagmin (Phospho-Thr202) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with Forskolin 40nM 30', using Synaptotagmin (Phospho-Thr202) Antibody. The lane on the left is blocked with the phospho peptide.
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SYT1 / Synaptotagmin Antibody (aa349-377, AP) LS-C224215
LifeSpan Biosciences
catalog: LS-C224215
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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SYT1 / Synaptotagmin Antibody (aa349-377, APC) LS-C224216
LifeSpan Biosciences
catalog: LS-C224216
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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SYT1 / Synaptotagmin Antibody (aa349-377, Biotin) LS-C224217
LifeSpan Biosciences
catalog: LS-C224217
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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SYT1 / Synaptotagmin Antibody (aa349-377, FITC) LS-C224218
LifeSpan Biosciences
catalog: LS-C224218
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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SYT1 / Synaptotagmin Antibody (aa349-377, Azide-free, HRP) LS-C224219
LifeSpan Biosciences
catalog: LS-C224219
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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SYT1 / Synaptotagmin Antibody (aa349-377, PE) LS-C224223
LifeSpan Biosciences
catalog: LS-C224223
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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SYT1 / Synaptotagmin Antibody LS-C285621
LifeSpan Biosciences
catalog: LS-C285621
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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SYT1 / Synaptotagmin Antibody (aa298) LS-C305894
LifeSpan Biosciences
catalog: LS-C305894
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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SYT1 / Synaptotagmin Antibody (Biotin) LS-C316767
LifeSpan Biosciences
catalog: LS-C316767
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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SYT1 / Synaptotagmin Antibody (HRP) LS-C316769
LifeSpan Biosciences
catalog: LS-C316769
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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SYT1 / Synaptotagmin Antibody (FITC) LS-C316770
LifeSpan Biosciences
catalog: LS-C316770
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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SYT1 / Synaptotagmin Antibody (aa349-377) LS-C325531
LifeSpan Biosciences
catalog: LS-C325531
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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SYT1 / Synaptotagmin Antibody (phospho-Ser309) LS-C336090
LifeSpan Biosciences
catalog: LS-C336090
domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry

Immunofluorescence staining of methanol-fixed HeLa cells.
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SYT1 / Synaptotagmin Antibody (clone ASV48) LS-C343669
LifeSpan Biosciences
catalog: LS-C343669
mouse monoclonal (ASV48)
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot of rat brain tissue extract, probed with Synaptotagmin monoclonal antibody (ASV48).
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SYT1 / Synaptotagmin Antibody LS-C348221
LifeSpan Biosciences
catalog: LS-C348221
domestic rabbit polyclonal
reactivity: human
application: western blot
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SYT1 / Synaptotagmin Antibody (Internal) LS-C354267
LifeSpan Biosciences
catalog: LS-C354267
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of Synaptotagmin expression in HEK293T (A); Jurkat (B) whole cell lysates.

Immunohistochemical analysis of Synaptotagmin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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IHC-plus™ SYT1 / Synaptotagmin Antibody LS-B12889
LifeSpan Biosciences
catalog: LS-B12889
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of extracts of various cell lines, using SYT1 antibody.

Immunofluorescence analysis of HeLa cell using SYT1 antibody. Blue: DAPI for nuclear staining.

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
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SYT1 / Synaptotagmin Antibody (Internal) LS-C358873
LifeSpan Biosciences
catalog: LS-C358873
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Synaptotagmin expression in HEK293T (A); mouse brain (B) whole cell lysates.

Immunohistochemical analysis of Synaptotagmin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Synaptotagmin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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