IHC-plus™ AURKA / Aurora-A Antibody (aa394-403) LS-B191
LifeSpan Biosciences
catalog: LS-B191

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-AURKA / Aurora-A antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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AURKA / Aurora-A Antibody (N-Terminus) LS-C3864
LifeSpan Biosciences
catalog: LS-C3864

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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AURKA / Aurora-A Antibody (N-Terminus, clone 35C1) LS-C44482
LifeSpan Biosciences
catalog: LS-C44482

mouse monoclonal (35C1)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

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AURKA / Aurora-A Antibody LS-C83024
LifeSpan Biosciences
catalog: LS-C83024

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

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AURKA / Aurora-A Antibody LS-C83025
LifeSpan Biosciences
catalog: LS-C83025

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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AURKA / Aurora-A Antibody (aa90-120) LS-C99458
LifeSpan Biosciences
catalog: LS-C99458

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of AIK (arrow) using rabbit polyclonal hAIK-H105. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AIK gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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IHC-plus™ AURKA / Aurora-A Antibody (clone 7A3) LS-B3912
LifeSpan Biosciences
catalog: LS-B3912

mouse monoclonal (7A3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Panel A Hela cells stained with anti-Aurora A (clone 7A3) and FITC-conjugate goat anti-mouse antibody. Panel B: Hela cells stained with DAPI to indicate nuclei.

Anti-AURKA / Aurora-A antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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AURKA / Aurora-A Antibody (aa256-305) LS-C117630
LifeSpan Biosciences
catalog: LS-C117630

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from 293 cells, treated with serum 20% 15', using Aurora Kinase Antibody. The lane on the right is blocked with the synthesized peptide.
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AURKA / Aurora-A Antibody (aa311-360) LS-C117995
LifeSpan Biosciences
catalog: LS-C117995

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from COS7 cells, using AurA Antibody. The lane on the right is blocked with the synthesized peptide.
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AURKA / Aurora-A Antibody (phospho-Thr288) LS-C122706
LifeSpan Biosciences
catalog: LS-C122706

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry

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AURKA / Aurora-A Antibody (aa1-111, clone 5F8) LS-C133953
LifeSpan Biosciences
catalog: LS-C133953

mouse monoclonal (5F8)
reactivity: human
application: western blot, ELISA, immunocytochemistry

STK6 monoclonal antibody (M01), clone 5F8 Western blot of STK6 expression in HeLa NE.

Immunofluorescence of monoclonal antibody to STK6 on HeLa cell. [antibody concentration 30 ug/ml]

Detection limit for recombinant GST tagged STK6 is approximately 0.1 ng/ml as a capture antibody.
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AURKA / Aurora-A Antibody LS-C137035
LifeSpan Biosciences
catalog: LS-C137035

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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IHC-plus™ AURKA / Aurora-A Antibody (aa100-150) LS-B6517
LifeSpan Biosciences
catalog: LS-B6517

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-AURKA / Aurora-A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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IHC-plus™ AURKA / Aurora-A Antibody (C-Terminus) LS-B6732
LifeSpan Biosciences
catalog: LS-B6732

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-AURKA / Aurora-A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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AURKA / Aurora-A Antibody (aa93-120) LS-C164298
LifeSpan Biosciences
catalog: LS-C164298

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Mouse Aurka Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The Aurka antibody detected the Aurka protein (arrow).
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AURKA / Aurora-A Antibody (clone 1F8) LS-C169310
LifeSpan Biosciences
catalog: LS-C169310

mouse monoclonal (1F8)
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry

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AURKA / Aurora-A Antibody (clone 4G10) LS-C169311
LifeSpan Biosciences
catalog: LS-C169311

mouse monoclonal (4G10)
reactivity: human
application: western blot, ELISA, immunohistochemistry

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AURKA / Aurora-A Antibody (Pro282) LS-C176486
LifeSpan Biosciences
catalog: LS-C176486

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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AURKA / Aurora-A Antibody LS-C180105
LifeSpan Biosciences
catalog: LS-C180105

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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AURKA / Aurora-A Antibody (phospho-Ser342) LS-C198659
LifeSpan Biosciences
catalog: LS-C198659

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using AurA (Phospho-Ser342) Antibody. The picture on the right is blocked with the phospho peptide.
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AURKA / Aurora-A Antibody (phospho-Thr288) LS-C198663
LifeSpan Biosciences
catalog: LS-C198663

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Aurora Kinase (Phospho-Thr288) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with serum 20% 15', using Aurora Kinase (Phospho-Thr288) Antibody. The lane on the right is blocked with the phospho peptide.
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AURKA / Aurora-A Antibody (aa151-200) LS-C201624
LifeSpan Biosciences
catalog: LS-C201624

domestic rabbit polyclonal
reactivity: human
application: western blot

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AURKA / Aurora-A Antibody (clone 3H1) LS-C204509
LifeSpan Biosciences
catalog: LS-C204509

mouse monoclonal (3H1)
reactivity: human
application: western blot, immunocytochemistry

Western analysis of AURKA / Aurora-A antibody. Blot of HeLa cells treated with 100 ng/mL of nocodazole for 18 hours was probed with AURKA / Aurora-A antibody. Nocodazole is a microtubule inhibitor which induces cells G2/M phase and induces Aurora A expression. The AURKA / Aurora-A antibody monoclonal binds strongly and cleanly to a band at about 46 kDa.

Shows HeLa cell cultures stained with AURKA / Aurora-A antibody antibody (green). Aurora A localizes in spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. Counterstained is a chicken polyclonal antibody against Vimentin (red). Blue is a DNA stain.
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AURKA / Aurora-A Antibody (aa93-120, AP) LS-C212873
LifeSpan Biosciences
catalog: LS-C212873

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: AP
application: western blot, ELISA

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AURKA / Aurora-A Antibody (aa93-120, APC) LS-C212874
LifeSpan Biosciences
catalog: LS-C212874

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: APC
application: western blot, ELISA

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AURKA / Aurora-A Antibody (aa93-120, Biotin) LS-C212875
LifeSpan Biosciences
catalog: LS-C212875

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA

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AURKA / Aurora-A Antibody (aa93-120, Azide-free, HRP) LS-C212876
LifeSpan Biosciences
catalog: LS-C212876

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA

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AURKA / Aurora-A Antibody (aa93-120, FITC) LS-C212877
LifeSpan Biosciences
catalog: LS-C212877

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA

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AURKA / Aurora-A Antibody (aa93-120, PE) LS-C212883
LifeSpan Biosciences
catalog: LS-C212883

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: PE
application: western blot, ELISA

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AURKA / Aurora-A Antibody (aa90-120, AP) LS-C218676
LifeSpan Biosciences
catalog: LS-C218676

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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AURKA / Aurora-A Antibody (aa90-120, APC) LS-C218677
LifeSpan Biosciences
catalog: LS-C218677

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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AURKA / Aurora-A Antibody (aa90-120, FITC) LS-C218678
LifeSpan Biosciences
catalog: LS-C218678

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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price:
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AURKA / Aurora-A Antibody (aa90-120, Azide-free, HRP) LS-C218679
LifeSpan Biosciences
catalog: LS-C218679

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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AURKA / Aurora-A Antibody (aa90-120, Biotin) LS-C218680
LifeSpan Biosciences
catalog: LS-C218680

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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AURKA / Aurora-A Antibody (aa90-120, PE) LS-C218682
LifeSpan Biosciences
catalog: LS-C218682

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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AURKA / Aurora-A Antibody (aa75-125) LS-C286339
LifeSpan Biosciences
catalog: LS-C286339

domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry

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AURKA / Aurora-A Antibody (phospho-Thr288) LS-C286340
LifeSpan Biosciences
catalog: LS-C286340

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunocytochemistry

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AURKA / Aurora-A Antibody (aa50-100) LS-C287008
LifeSpan Biosciences
catalog: LS-C287008

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

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AURKA / Aurora-A Antibody (aa75-125) LS-C287010
LifeSpan Biosciences
catalog: LS-C287010

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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AURKA / Aurora-A Antibody (aa109-125) LS-C312934
LifeSpan Biosciences
catalog: LS-C312934

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

WB of AURKA / Aurora-A antibody. Lane 1: SKOV Cell Lysate. Lane 2: 22RV1 Cell Lysate. Lane 3: HELA Cell Lysate . Lane 4: JURKAT Cell Lysate.
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AURKA / Aurora-A Antibody LS-C193071
LifeSpan Biosciences
catalog: LS-C193071

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of various cell lines, using AURKA antibody.
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AURKA / Aurora-A Antibody (aa90-120) LS-C326899
LifeSpan Biosciences
catalog: LS-C326899

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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AURKA / Aurora-A Antibody (aa93-120) LS-C329244
LifeSpan Biosciences
catalog: LS-C329244

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

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AURKA / Aurora-A Antibody LS-C331913
LifeSpan Biosciences
catalog: LS-C331913

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines.
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AURKA / Aurora-A Antibody (phospho-Thr288) LS-C335956
LifeSpan Biosciences
catalog: LS-C335956

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
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AURKA / Aurora-A Antibody (phospho-Thr288) LS-C340882
LifeSpan Biosciences
catalog: LS-C340882

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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AURKA / Aurora-A Antibody (N-Terminus) LS-C342006
LifeSpan Biosciences
catalog: LS-C342006

mouse monoclonal
reactivity: human
application: western blot

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AURKA / Aurora-A Antibody (clone 1364CT291.108.155) LS-C344157
LifeSpan Biosciences
catalog: LS-C344157

mouse monoclonal (1364CT291.108.155)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemical of paraffin-embedded H. brain section using AURKA Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot of lysate from HT-29 cell line with AURKA Antibody. Antibody was diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35 ug.
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AURKA / Aurora-A Antibody (C-Terminus) LS-C354094
LifeSpan Biosciences
catalog: LS-C354094

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Aurora A expression in MCF7 (A); SP2/0 (B); H9C2 (C) whole cell lysates.

Immunohistochemical analysis of Aurora A staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Aurora A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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AURKA / Aurora-A Antibody (phospho-Thr288) LS-C358422
LifeSpan Biosciences
catalog: LS-C358422

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Aurora A (pT288) expression in DLD (A); mouse brain (B); rat brain (C) whole cell lysates.

Immunohistochemical analysis of Aurora A (pT288) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Aurora A (pT288) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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AURKA / Aurora-A Antibody (clone 1F8) LS-C359318
LifeSpan Biosciences
catalog: LS-C359318

mouse monoclonal (1F8)
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry

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AURKA / Aurora-A Antibody (230-310 aa) LS-C381244
LifeSpan Biosciences
catalog: LS-C381244

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-ARK-1 (T288) antibody
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AURKA / Aurora-A Antibody (280-360 aa) LS-C381614
LifeSpan Biosciences
catalog: LS-C381614

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry

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AURKA / Aurora-A Antibody (230-310 aa) LS-C382138
LifeSpan Biosciences
catalog: LS-C382138

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of ARK-1 antibody
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AURKA / Aurora-A Antibody (280-360 aa) LS-C382140
LifeSpan Biosciences
catalog: LS-C382140

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of ARK-1 antibody
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