IHC-plus™ SPP1 / Osteopontin Antibody (aa288-299) LS-B2411
LifeSpan Biosciences
catalog: LS-B2411
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

SPP1 / Osteopontin antibody (0.5µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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SPP1 / Osteopontin Antibody (C-Terminus) LS-C31763
LifeSpan Biosciences
catalog: LS-C31763
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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PathPlus™ SPP1 / Osteopontin Antibody LS-B425
LifeSpan Biosciences
catalog: LS-B425
domestic rabbit polyclonal
reactivity: human, dogs
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 1

Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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SPP1 / Osteopontin Antibody (aa75-90) LS-C9410
LifeSpan Biosciences
catalog: LS-C9410
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section
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SPP1 / Osteopontin Antibody LS-C9413
LifeSpan Biosciences
catalog: LS-C9413
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
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SPP1 / Osteopontin Antibody (clone 53) LS-C63081
LifeSpan Biosciences
catalog: LS-C63081
mouse monoclonal (53)
reactivity: human
application: western blot
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SPP1 / Osteopontin Antibody (C-Terminus, clone 87-B) LS-C63082
LifeSpan Biosciences
catalog: LS-C63082
mouse monoclonal (87-B)
reactivity: human
application: western blot
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SPP1 / Osteopontin Antibody (aa17-314) LS-C73947
LifeSpan Biosciences
catalog: LS-C73947
mouse monoclonal
reactivity: human
application: ELISA
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SPP1 / Osteopontin Antibody LS-C88774
LifeSpan Biosciences
catalog: LS-C88774
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed, paraffin-embedded human osteosarcoma stained with peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
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SPP1 / Osteopontin Antibody LS-C96393
LifeSpan Biosciences
catalog: LS-C96393
domestic rabbit polyclonal
reactivity: human, mouse
application: immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
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SPP1 / Osteopontin Antibody (aa273-301) LS-C99283
LifeSpan Biosciences
catalog: LS-C99283
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of SPP1 Antibody in NCI-H460 cell line lysates (35 ug/lane). SPP1 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric of NCI-H292 cells using SPP1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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SPP1 / Osteopontin Antibody (aa59-74) LS-C122259
LifeSpan Biosciences
catalog: LS-C122259
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
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SPP1 / Osteopontin Antibody (aa164-179) LS-C131159
LifeSpan Biosciences
catalog: LS-C131159
domestic rabbit polyclonal
reactivity: human
application: ELISA
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SPP1 / Osteopontin Antibody (PE) LS-C131160
LifeSpan Biosciences
catalog: LS-C131160
domestic goat polyclonal
reactivity: human, mouse
conjugate: PE
application: flow cytometry
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IHC-plus™ SPP1 / Osteopontin Antibody (Internal) LS-B5272
LifeSpan Biosciences
catalog: LS-B5272
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:25.
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IHC-plus™ SPP1 / Osteopontin Antibody (N-Terminus) LS-B5626
LifeSpan Biosciences
catalog: LS-B5626
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-Osteopontin antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC-plus™ SPP1 / Osteopontin Antibody (clone X1) LS-B5940
LifeSpan Biosciences
catalog: LS-B5940
mouse monoclonal (X1)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Detection limit for recombinant GST tagged SPP1 is approximately 0.3 ng/ml as a capture antibody.

Anti-Osteopontin antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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SPP1 / Osteopontin Antibody LS-C136850
LifeSpan Biosciences
catalog: LS-C136850
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (aa17-314) LS-C137501
LifeSpan Biosciences
catalog: LS-C137501
mouse monoclonal
reactivity: human
application: western blot, ELISA
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IHC-plus™ SPP1 / Osteopontin Antibody (C-Terminus, clone 1B20) LS-B8326
LifeSpan Biosciences
catalog: LS-B8326
mouse monoclonal (1B20)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (clone 7C5H12) LS-C169155
LifeSpan Biosciences
catalog: LS-C169155
mouse monoclonal (7C5H12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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SPP1 / Osteopontin Antibody (Leu285) LS-C176152
LifeSpan Biosciences
catalog: LS-C176152
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of Osteopontin/SPP1 (L285) pAb in extracts from LOVO cells.
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SPP1 / Osteopontin Antibody (clone 1B20, Biotin) LS-C180345
LifeSpan Biosciences
catalog: LS-C180345
mouse monoclonal (1B20)
reactivity: human
conjugate: biotin
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (clone 1B20, DY650) LS-C180346
LifeSpan Biosciences
catalog: LS-C180346
mouse monoclonal (1B20)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (clone 1B20, DY488) LS-C180347
LifeSpan Biosciences
catalog: LS-C180347
mouse monoclonal (1B20)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (clone 1B20, DY550) LS-C180349
LifeSpan Biosciences
catalog: LS-C180349
mouse monoclonal (1B20)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (clone 11n145, CF) LS-C184863
LifeSpan Biosciences
catalog: LS-C184863
mouse monoclonal (11n145)
reactivity: human
application: flow cytometry
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SPP1 / Osteopontin Antibody (clone 11n145, PE) LS-C184864
LifeSpan Biosciences
catalog: LS-C184864
mouse monoclonal (11n145)
reactivity: human
conjugate: PE
application: flow cytometry
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SPP1 / Osteopontin Antibody (clone 12K149, APC) LS-C182923
LifeSpan Biosciences
catalog: LS-C182923
mouse monoclonal (12K149)
reactivity: human
conjugate: APC
application: flow cytometry
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IHC-plus™ SPP1 / Osteopontin Antibody (aa261-310) LS-B9080
LifeSpan Biosciences
catalog: LS-B9080
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-SPP1 / Osteopontin antibody IHC staining of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot analysis of lysates from LOVO cells, using Osteopontin Antibody. The lane on the right is blocked with the synthesized peptide.
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SPP1 / Osteopontin Antibody (clone 1F11) LS-C189633
LifeSpan Biosciences
catalog: LS-C189633
mouse monoclonal (1F11)
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (clone 2F10) LS-C189634
LifeSpan Biosciences
catalog: LS-C189634
mouse monoclonal (2F10)
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (clone 2C5) LS-C189635
LifeSpan Biosciences
catalog: LS-C189635
mouse monoclonal (2C5)
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (clone 2H9) LS-C189636
LifeSpan Biosciences
catalog: LS-C189636
mouse monoclonal (2H9)
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (clone 2E11) LS-C189637
LifeSpan Biosciences
catalog: LS-C189637
mouse monoclonal (2E11)
reactivity: human
application: western blot, ELISA
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PathPlus™ SPP1 / Osteopontin Antibody (aa14-40) LS-B9287
LifeSpan Biosciences
catalog: LS-B9287
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-SPP1 / Osteopontin antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot of OPN-a/b (arrow) using rabbit polyclonal OPN-a/b Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OPN-a/b gene.

Confocal immunofluorescence of OPN-a/b Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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SPP1 / Osteopontin Antibody LS-C211985
LifeSpan Biosciences
catalog: LS-C211985
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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SPP1 / Osteopontin Antibody (HRP) LS-C211986
LifeSpan Biosciences
catalog: LS-C211986
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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SPP1 / Osteopontin Antibody (FITC) LS-C211987
LifeSpan Biosciences
catalog: LS-C211987
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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SPP1 / Osteopontin Antibody (aa17-302, Biotin) LS-C211989
LifeSpan Biosciences
catalog: LS-C211989
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA
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SPP1 / Osteopontin Antibody LS-C212311
LifeSpan Biosciences
catalog: LS-C212311
mouse monoclonal
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (aa14-40, AP) LS-C216180
LifeSpan Biosciences
catalog: LS-C216180
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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SPP1 / Osteopontin Antibody (aa14-40, APC) LS-C216181
LifeSpan Biosciences
catalog: LS-C216181
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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SPP1 / Osteopontin Antibody (aa14-40, Biotin) LS-C216182
LifeSpan Biosciences
catalog: LS-C216182
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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SPP1 / Osteopontin Antibody (aa14-40, FITC) LS-C216183
LifeSpan Biosciences
catalog: LS-C216183
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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SPP1 / Osteopontin Antibody (aa14-40, Azide-free, HRP) LS-C216184
LifeSpan Biosciences
catalog: LS-C216184
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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to the supplier
SPP1 / Osteopontin Antibody (aa14-40, PE) LS-C216188
LifeSpan Biosciences
catalog: LS-C216188
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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PathPlus™ SPP1 / Osteopontin Antibody LS-B10122
LifeSpan Biosciences
catalog: LS-B10122
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at (A) 1 and (B) 2 ug/ml.

Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 ug/ml.

Anti-SPP1 / Osteopontin antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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SPP1 / Osteopontin Antibody (aa7-232) LS-C295741
LifeSpan Biosciences
catalog: LS-C295741
domestic rabbit polyclonal
reactivity: human, dogs
application: western blot

Western blot of recombinant SPP1 / Osteopontin. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (aa17-300) LS-C295742
LifeSpan Biosciences
catalog: LS-C295742
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant SPP1 / Osteopontin. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (aa17-300, Biotin) LS-C300677
LifeSpan Biosciences
catalog: LS-C300677
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western blot of recombinant SPP1 / Osteopontin. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (aa7-232, Biotin) LS-C300678
LifeSpan Biosciences
catalog: LS-C300678
domestic rabbit polyclonal
reactivity: human, dogs
conjugate: biotin
application: western blot

Western blot of recombinant SPP1 / Osteopontin. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (aa17-300, FITC) LS-C304285
LifeSpan Biosciences
catalog: LS-C304285
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot

Western blot of recombinant SPP1 / Osteopontin. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (clone 14C08) LS-C305904
LifeSpan Biosciences
catalog: LS-C305904
mouse monoclonal (14C08)
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (clone 2C5) LS-C305906
LifeSpan Biosciences
catalog: LS-C305906
mouse monoclonal (2C5)
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (clone 2F10) LS-C305907
LifeSpan Biosciences
catalog: LS-C305907
mouse monoclonal (2F10)
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (clone 2H9) LS-C305909
LifeSpan Biosciences
catalog: LS-C305909
mouse monoclonal (2H9)
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (clone 2E11) LS-C305910
LifeSpan Biosciences
catalog: LS-C305910
mouse monoclonal (2E11)
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (clone 1F11) LS-C305911
LifeSpan Biosciences
catalog: LS-C305911
mouse monoclonal (1F11)
reactivity: human
application: western blot, ELISA
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SPP1 / Osteopontin Antibody (aa28-47) LS-C312595
LifeSpan Biosciences
catalog: LS-C312595
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

WB of SPP1 / Osteopontin antibody. Lane 1: MM231 Cell Lysate. Lane 2: HELA Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4: COLO320 Cell Lysate.

SPP1 / Osteopontin antibody. IHC(P): Human Breast Cancer Tissue.
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SPP1 / Osteopontin Antibody (aa17-287) LS-C314656
LifeSpan Biosciences
catalog: LS-C314656
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant SPP1 / Osteopontin.

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SPP1 / Osteopontin Antibody (HRP) LS-C317847
LifeSpan Biosciences
catalog: LS-C317847
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA

Western blot of Osteopontin antibody at 2 ug/ml with mouse kidney tissue Secondary Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 32 KDa. Observed band size: 32 KDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (Biotin) LS-C317849
LifeSpan Biosciences
catalog: LS-C317849
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA

Western blot of Osteopontin antibody at 2 ug/ml with mouse kidney tissue Secondary Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 32 KDa. Observed band size: 32 KDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody LS-C318503
LifeSpan Biosciences
catalog: LS-C318503
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

Western blot of Osteopontin antibody at 2 ug/ml with mouse kidney tissue Secondary Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 32 KDa. Observed band size: 32 KDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (FITC) LS-C318965
LifeSpan Biosciences
catalog: LS-C318965
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA

Western blot of Osteopontin antibody at 2 ug/ml with mouse kidney tissue Secondary Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 32 KDa. Observed band size: 32 KDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPP1 / Osteopontin Antibody (aa14-40) LS-C323539
LifeSpan Biosciences
catalog: LS-C323539
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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SPP1 / Osteopontin Antibody LS-C331430
LifeSpan Biosciences
catalog: LS-C331430
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of mouse brain, using SPP1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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SPP1 / Osteopontin Antibody (aa17-314, clone OTI6C12) LS-C339892
LifeSpan Biosciences
catalog: LS-C339892
mouse monoclonal (OTI6C12)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1.
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SPP1 / Osteopontin Antibody (aa17-314, clone OTI6A12) LS-C339909
LifeSpan Biosciences
catalog: LS-C339909
mouse monoclonal (OTI6A12)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1.
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SPP1 / Osteopontin Antibody (aa17-314, clone OTI2F2) LS-C340065
LifeSpan Biosciences
catalog: LS-C340065
mouse monoclonal (OTI2F2)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1.

IHC of paraffin-embedded Human colon tissue using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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SPP1 / Osteopontin Antibody (aa17-314, clone OTI3C4) LS-C340079
LifeSpan Biosciences
catalog: LS-C340079
mouse monoclonal (OTI3C4)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1.
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SPP1 / Osteopontin Antibody (aa17-314, clone OTI5E4) LS-C340085
LifeSpan Biosciences
catalog: LS-C340085
mouse monoclonal (OTI5E4)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1.

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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SPP1 / Osteopontin Antibody (230-310 aa, C-terminal) LS-C385263
LifeSpan Biosciences
catalog: LS-C385263
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of OPN antibody
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