SPI1 / PU.1 Antibody (N-Terminus) LS-C38665
LifeSpan Biosciences
catalog: LS-C38665

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

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IHC-plus™ SPI1 / PU.1 Antibody (aa27-37) LS-B3822
LifeSpan Biosciences
catalog: LS-B3822

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-SPI1 / PU.1 antibody IHC of human colon, lymphoid aggregate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

SPI1 / PU.1 antibody (0.03µg/ml) staining of DAUDI lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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IHC-plus™ SPI1 / PU.1 Antibody LS-B4206
LifeSpan Biosciences
catalog: LS-B4206

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: Hela. 12% SDS PAGE. SPI1 antibody diluted at 1:1000.

Anti-SPI1 / PU.1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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SPI1 / PU.1 Antibody (aa61-110) LS-C30679
LifeSpan Biosciences
catalog: LS-C30679

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, reverse phase protein lysate microarray
citations: 1

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SPI1 / PU.1 Antibody (aa104-153) LS-C30035
LifeSpan Biosciences
catalog: LS-C30035

domestic rabbit polyclonal
reactivity: human
application: western blot

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SPI1 / PU.1 Antibody (N-Terminus) LS-C46838
LifeSpan Biosciences
catalog: LS-C46838

domestic rabbit polyclonal
reactivity: human, mouse
application: ELISA

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SPI1 / PU.1 Antibody (C-Terminus) LS-C101683
LifeSpan Biosciences
catalog: LS-C101683

domestic rabbit polyclonal
reactivity: human
application: western blot

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SPI1 / PU.1 Antibody (aa181-230) LS-C118804
LifeSpan Biosciences
catalog: LS-C118804

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from COS7 cells, using SPI1 Antibody. The lane on the right is blocked with the synthesized peptide.
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SPI1 / PU.1 Antibody (PE) LS-C144123
LifeSpan Biosciences
catalog: LS-C144123

domestic sheep polyclonal
reactivity: human
conjugate: PE
application: flow cytometry

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SPI1 / PU.1 Antibody (clone 5C7) LS-C169103
LifeSpan Biosciences
catalog: LS-C169103

mouse monoclonal (5C7)
reactivity: human
application: western blot, ELISA, flow cytometry

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SPI1 / PU.1 Antibody (N-Terminus) LS-C170148
LifeSpan Biosciences
catalog: LS-C170148

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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SPI1 / PU.1 Antibody (Arg212) LS-C177119
LifeSpan Biosciences
catalog: LS-C177119

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of PU.1 (R212) pAb in extracts from COS-7 cells.
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IHC-plus™ SPI1 / PU.1 Antibody (aa217-246) LS-B9322
LifeSpan Biosciences
catalog: LS-B9322

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-SPI1 / PU.1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody. NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SPI1 immunoreactivity is localized to Nucleus significantly.

SPI1 Antibody western blot of WiDr cell line lysates (35 ug/lane). The SPI1 antibody detected the SPI1 protein (arrow).
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SPI1 / PU.1 Antibody (clone 1A3) LS-C198253
LifeSpan Biosciences
catalog: LS-C198253

mouse monoclonal (1A3)
reactivity: human
application: western blot, ELISA

Western Blot analysis of SPI1 expression in transfected 293T cell line by SPI1 monoclonal antibody (M01), clone 1A3.Lane 1: SPI1 transfected lysate(31.083 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged SPI1 is approximately 10 ng/ml as a capture antibody.
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SPI1 / PU.1 Antibody (aa163-212) LS-C200715
LifeSpan Biosciences
catalog: LS-C200715

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HUVECcells using SPI1 antibody.
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SPI1 / PU.1 Antibody (N-Terminus) LS-C201234
LifeSpan Biosciences
catalog: LS-C201234

domestic rabbit polyclonal
reactivity: human
application: western blot

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SPI1 / PU.1 Antibody LS-C203294
LifeSpan Biosciences
catalog: LS-C203294

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of human recombinant PU.1 fusion protein using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 62 kD Observed Size: 62 kD
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SPI1 / PU.1 Antibody (aa217-246, AP) LS-C270188
LifeSpan Biosciences
catalog: LS-C270188

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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SPI1 / PU.1 Antibody (aa217-246, APC) LS-C270191
LifeSpan Biosciences
catalog: LS-C270191

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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SPI1 / PU.1 Antibody (aa217-246, Biotin) LS-C270192
LifeSpan Biosciences
catalog: LS-C270192

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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SPI1 / PU.1 Antibody (aa217-246, Azide-free, HRP) LS-C270193
LifeSpan Biosciences
catalog: LS-C270193

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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SPI1 / PU.1 Antibody (aa217-246, FITC) LS-C270194
LifeSpan Biosciences
catalog: LS-C270194

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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SPI1 / PU.1 Antibody (aa217-246, PE) LS-C270195
LifeSpan Biosciences
catalog: LS-C270195

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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SPI1 / PU.1 Antibody (Biotin) LS-C317497
LifeSpan Biosciences
catalog: LS-C317497

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPI1 / PU.1 Antibody (FITC) LS-C317499
LifeSpan Biosciences
catalog: LS-C317499

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPI1 / PU.1 Antibody (HRP) LS-C317500
LifeSpan Biosciences
catalog: LS-C317500

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPI1 / PU.1 Antibody (aa217-246) LS-C325330
LifeSpan Biosciences
catalog: LS-C325330

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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SPI1 / PU.1 Antibody (Internal) LS-C358867
LifeSpan Biosciences
catalog: LS-C358867

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of PU.1 expression in HepG2 (A); TF1 (B) whole cell lysates.

Immunohistochemical analysis of PU.1 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PU.1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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IHC-plus™ SPI1 / PU.1 Antibody LS-B13006
LifeSpan Biosciences
catalog: LS-B13006

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPI1 / PU.1 Antibody (150-230 aa, Internal) LS-C385679
LifeSpan Biosciences
catalog: LS-C385679

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of PU.1 antibody
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