Osteonectin / SPARC Antibody LS-C25802
LifeSpan Biosciences
catalog: LS-C25802
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section
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Osteonectin / SPARC Antibody (clone ON1-1) LS-C25804
LifeSpan Biosciences
catalog: LS-C25804
mouse monoclonal (ON1-1)
reactivity: human, bovine
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Osteonectin / SPARC Antibody (clone 5031) LS-C25800
LifeSpan Biosciences
catalog: LS-C25800
mouse monoclonal (5031)
reactivity: human
application: western blot, ELISA, immunohistochemistry, radioimmunoassay
citations: 1
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Osteonectin / SPARC Antibody LS-C26929
LifeSpan Biosciences
catalog: LS-C26929
domestic rabbit polyclonal
reactivity: human
application: western blot
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Osteonectin / SPARC Antibody (clone ON1-1) LS-C83590
LifeSpan Biosciences
catalog: LS-C83590
mouse monoclonal (ON1-1)
reactivity: human, bovine
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
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Osteonectin / SPARC Antibody LS-C122256
LifeSpan Biosciences
catalog: LS-C122256
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section
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Osteonectin / SPARC Antibody (aa272-286) LS-C124319
LifeSpan Biosciences
catalog: LS-C124319
domestic rabbit polyclonal
reactivity: human
application: ELISA
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Osteonectin / SPARC Antibody LS-C124320
LifeSpan Biosciences
catalog: LS-C124320
rat monoclonal
reactivity: human, mouse
application: ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
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Osteonectin / SPARC Antibody LS-C131158
LifeSpan Biosciences
catalog: LS-C131158
domestic goat polyclonal
reactivity: human, mouse
application: western blot, ELISA, flow cytometry
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Osteonectin / SPARC Antibody (aa224-251) LS-C156115
LifeSpan Biosciences
catalog: LS-C156115
mouse monoclonal
reactivity: human
application: western blot

SPARC Antibody (Ascites)western blot of HeLa cell line lysates (35 ug/lane). The SPARC antibody detected the SPARC protein (arrow).
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Osteonectin / SPARC Antibody (aa224-251) LS-C161424
LifeSpan Biosciences
catalog: LS-C161424
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of SPARC (arrow) using rabbit polyclonal SPARC Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.

Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric of widr cells using SPARC Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Osteonectin / SPARC Antibody (Gly53) LS-C191122
LifeSpan Biosciences
catalog: LS-C191122
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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Osteonectin / SPARC Antibody (clone 1B2) LS-C198252
LifeSpan Biosciences
catalog: LS-C198252
mouse monoclonal (1B2)
reactivity: human
application: western blot, ELISA

Western Blot analysis of SPARC expression in transfected 293T cell line by SPARC monoclonal antibody (M02), clone 1B2.Lane 1: SPARC transfected lysate(34.6 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged SPARC is approximately 3 ng/ml as a capture antibody.
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Osteonectin / SPARC Antibody (aa224-251, APC) LS-C280445
LifeSpan Biosciences
catalog: LS-C280445
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Osteonectin / SPARC Antibody (aa224-251, AP) LS-C280446
LifeSpan Biosciences
catalog: LS-C280446
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Osteonectin / SPARC Antibody (aa224-251, Biotin) LS-C280447
LifeSpan Biosciences
catalog: LS-C280447
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, immunohistochemistry, flow cytometry
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Osteonectin / SPARC Antibody (aa224-251, FITC) LS-C280448
LifeSpan Biosciences
catalog: LS-C280448
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Osteonectin / SPARC Antibody (aa224-251, Azide-free, HRP) LS-C280449
LifeSpan Biosciences
catalog: LS-C280449
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Osteonectin / SPARC Antibody (aa224-251, PE) LS-C280450
LifeSpan Biosciences
catalog: LS-C280450
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Osteonectin / SPARC Antibody (aa1-304, Biotin) LS-C280453
LifeSpan Biosciences
catalog: LS-C280453
mouse monoclonal
reactivity: human
conjugate: biotin
application: western blot
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Osteonectin / SPARC Antibody (aa18-302) LS-C295737
LifeSpan Biosciences
catalog: LS-C295737
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western Blot; Sample: Mouse Lung lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse ON Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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Osteonectin / SPARC Antibody (aa18-301) LS-C295738
LifeSpan Biosciences
catalog: LS-C295738
domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot of recombinant Osteonectin / SPARC. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Osteonectin / SPARC Antibody (aa18-302, Biotin) LS-C297412
LifeSpan Biosciences
catalog: LS-C297412
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot

Western Blot; Sample: Mouse Lung lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse ON Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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Osteonectin / SPARC Antibody (aa18-301, Biotin) LS-C297413
LifeSpan Biosciences
catalog: LS-C297413
domestic rabbit polyclonal
reactivity: human, rat
conjugate: biotin
application: western blot

Western blot of recombinant Osteonectin / SPARC. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Osteonectin / SPARC Antibody (aa18-302, FITC) LS-C301772
LifeSpan Biosciences
catalog: LS-C301772
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot

Western Blot; Sample: Mouse Lung lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse ON Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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Osteonectin / SPARC Antibody (aa18-301, FITC) LS-C301774
LifeSpan Biosciences
catalog: LS-C301774
domestic rabbit polyclonal
reactivity: human, rat
conjugate: FITC
application: western blot

Western blot of recombinant Osteonectin / SPARC. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Osteonectin / SPARC Antibody (aa284-303) LS-C312741
LifeSpan Biosciences
catalog: LS-C312741
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

WB of Osteonectin / SPARC antibody. All lanes: Anti-SPARC at 0.5ug/ml. WB: HELA Whole Cell Lysate at 40ug. Predicted bind size: 35KD. Observed bind size: 35KD.

Osteonectin / SPARC antibody. IHC(P): Human Intestinal Cancer Tissue.
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Osteonectin / SPARC Antibody LS-C192907
LifeSpan Biosciences
catalog: LS-C192907
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of Y79 cell lines, using SPARC antibody.
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Osteonectin / SPARC Antibody (aa1-304, Azide-free) LS-C328105
LifeSpan Biosciences
catalog: LS-C328105
mouse monoclonal
reactivity: human
application: western blot
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Osteonectin / SPARC Antibody (aa224-251) LS-C328107
LifeSpan Biosciences
catalog: LS-C328107
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry
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PathPlus™ Osteonectin / SPARC Antibody (C-Terminus) LS-B11553
LifeSpan Biosciences
catalog: LS-B11553
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Osteonectin / SPARC Antibody LS-C331587
LifeSpan Biosciences
catalog: LS-C331587
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Immunohistochemistry of paraffin-embedded rat kidney using SPARC antibody at dilution of 1:100 (200x lens).

Western blot analysis of extracts of various cell lines, using SPARC antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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Osteonectin / SPARC Antibody (clone OTI1D6) LS-C338482
LifeSpan Biosciences
catalog: LS-C338482
mouse monoclonal (OTI1D6)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPARC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPARC.
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Osteonectin / SPARC Antibody LS-C340895
LifeSpan Biosciences
catalog: LS-C340895
domestic rabbit polyclonal
reactivity: human
application: western blot
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Osteonectin / SPARC Antibody (Internal) LS-C353788
LifeSpan Biosciences
catalog: LS-C353788
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of SPARC expression in A549 (A); NIH3T3 (B); PC12 (C) whole cell lysates.
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Osteonectin / SPARC Antibody (aa46-63) LS-C359463
LifeSpan Biosciences
catalog: LS-C359463
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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PathPlus™ Osteonectin / SPARC Antibody (clone 2B1) LS-B13205
LifeSpan Biosciences
catalog: LS-B13205
mouse monoclonal (2B1)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPARC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPARC.

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
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