IHC-plus™ SP1 Antibody (Internal) LS-B1647
LifeSpan Biosciences
catalog: LS-B1647
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-SP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Sp1 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
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Nuclei and Chromosomes, Histone specific Antibody LS-C122249
LifeSpan Biosciences
catalog: LS-C122249
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
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SP1 Antibody (phospho-Thr739) LS-C117476
LifeSpan Biosciences
catalog: LS-C117476
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from COS7 cells treated with serum 20% 15', using SP1 (Phospho-Thr739) Antibody. The lane on the right is blocked with the phospho peptide.
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IHC-plus™ SP1 Antibody (clone 1A5) LS-B6148
LifeSpan Biosciences
catalog: LS-B6148
mouse monoclonal (1A5)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

SP1 monoclonal antibody (M02), clone 1A5 Western blot of SP1 expression in HeLa NE.

Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged SP1 is approximately 0.1 ng/ml as a capture antibody.
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SP1 Antibody (aa68-82) LS-C20214
LifeSpan Biosciences
catalog: LS-C20214
domestic goat polyclonal
reactivity: human
application: western blot

SP1 antibody staining (0.3ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
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SP1 Antibody (aa550-599) LS-C31420
LifeSpan Biosciences
catalog: LS-C31420
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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SP1 Antibody (aa347-396) LS-C31115
LifeSpan Biosciences
catalog: LS-C31115
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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SP1 Antibody LS-C41576
LifeSpan Biosciences
catalog: LS-C41576
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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SP1 Antibody (aa501-550) LS-C80591
LifeSpan Biosciences
catalog: LS-C80591
domestic rabbit polyclonal
reactivity: human
application: western blot
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SP1 Antibody (aa706-755) LS-C118246
LifeSpan Biosciences
catalog: LS-C118246
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of HepG2 cells, using SP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HT-29 cells, using SP1 Antibody. The lane on the right is blocked with the synthesized peptide.
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SP3+4 Antibody (aa671-720) LS-C118978
LifeSpan Biosciences
catalog: LS-C118978
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from Jurkat cells, using SP3/4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Prostate Specific Gene-1 (HPG-1) Antibody LS-C131660
LifeSpan Biosciences
catalog: LS-C131660
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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SP1 Antibody (clone 4H6) LS-C133444
LifeSpan Biosciences
catalog: LS-C133444
mouse monoclonal (4H6)
reactivity: human
application: western blot, ELISA, immunocytochemistry

SP1 monoclonal antibody (M01), clone 4H6 Western blot of SP1 expression in IMR-32.

Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]
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SP1 Antibody LS-C136946
LifeSpan Biosciences
catalog: LS-C136946
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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SP1 Antibody LS-C137189
LifeSpan Biosciences
catalog: LS-C137189
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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IHC-plus™ SP1 Antibody (clone 4C8) LS-B6149
LifeSpan Biosciences
catalog: LS-B6149
mouse monoclonal (4C8)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of SP1 expression in HeLa NE.

Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 20 ug/ml]

Detection limit for recombinant GST tagged SP1 is approximately 0.3 ng/ml as a capture antibody.
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IHC-plus™ SP1 Antibody (aa421-470) LS-B7624
LifeSpan Biosciences
catalog: LS-B7624
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-SP1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of A549 cells, using SP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from LOVO cells, using SP1 Antibody. The lane on the right is blocked with the synthesized peptide.
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SP1 Antibody (aa677-707) LS-C161407
LifeSpan Biosciences
catalog: LS-C161407
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

SP1 Antibody (C-term P692) western blot of K562 cell line lysates (35 ug/lane). The SP1 antibody detected the SP1 protein (arrow).

SP1 Antibody (C-term P692) immunohistochemistry of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

SP1 Antibody (C-term P692) flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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SP1 Antibody (Gly447) LS-C176472
LifeSpan Biosciences
catalog: LS-C176472
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of SP1 (G447) pAb in K-562 (A) and A-431 (B) whole cell lysates.

IHC of SP1 (G447) pAb in paraffin-embedded human brain tissue.
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SP1 Antibody (Glu336) LS-C177670
LifeSpan Biosciences
catalog: LS-C177670
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of SP1 (E336) pAb in K-562 (A) and A-431 (B) whole cell lysates.
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SP1 Antibody (clone SP15H09) LS-C184232
LifeSpan Biosciences
catalog: LS-C184232
mouse monoclonal (SP15H09)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
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SP1 Antibody (aa385-433) LS-C187104
LifeSpan Biosciences
catalog: LS-C187104
domestic rabbit polyclonal
reactivity: human
application: western blot
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SP1 Antibody (phospho-Thr453) LS-C199597
LifeSpan Biosciences
catalog: LS-C199597
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HeLa cells, using SP1 (Phospho-Thr453) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using SP1 (Phospho-Thr453) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from A549 cells, using SP1 (Phospho-Thr453) Antibody. The lane on the right is blocked with the phospho peptide.
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SP1 Antibody (aa677-707, AP) LS-C270043
LifeSpan Biosciences
catalog: LS-C270043
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
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SP1 Antibody (aa677-707, APC) LS-C270044
LifeSpan Biosciences
catalog: LS-C270044
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
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SP1 Antibody (aa677-707, Biotin) LS-C270045
LifeSpan Biosciences
catalog: LS-C270045
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
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SP1 Antibody (aa677-707, Azide-free, HRP) LS-C270046
LifeSpan Biosciences
catalog: LS-C270046
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
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SP1 Antibody (aa677-707, FITC) LS-C270047
LifeSpan Biosciences
catalog: LS-C270047
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
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SP1 Antibody (aa677-707, PE) LS-C270049
LifeSpan Biosciences
catalog: LS-C270049
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
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SP1 Antibody (aa750-C-term) LS-C286457
LifeSpan Biosciences
catalog: LS-C286457
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
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SP1 Antibody (aa700-740) LS-C287062
LifeSpan Biosciences
catalog: LS-C287062
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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SP1 Antibody (aa750-C-term) LS-C287063
LifeSpan Biosciences
catalog: LS-C287063
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, ChIP-Seq
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SP1 Antibody (aa572-585) LS-C305884
LifeSpan Biosciences
catalog: LS-C305884
domestic goat polyclonal
reactivity: human, mouse
application: western blot

SP1 antibody (0.3 ug/ml) staining of NIH3T3 nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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SP1 Antibody LS-C332498
LifeSpan Biosciences
catalog: LS-C332498
mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Western blot analysis of extracts of Jurkat cells.
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SP1 Antibody (clone SP15H09) LS-C342298
LifeSpan Biosciences
catalog: LS-C342298
mouse monoclonal (SP15H09)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Western blot analysis of immunized recombinant protein, using anti-SP1 monoclonal antibody.

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SP1 mAb.
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SP1 Antibody (clone 1326CT463.109.176) LS-C344150
LifeSpan Biosciences
catalog: LS-C344150
mouse monoclonal (1326CT463.109.176)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Flow cytometric of HeLa cells with SP1 Antibody (green) compared to an isotype control of mouse IgG1 (blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Fluorescent image of HeLa cells stained with SP1 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot of lysates from HeLa, Ramos cell line (from left to right) with SP1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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SP1 Antibody (Internal) LS-C354082
LifeSpan Biosciences
catalog: LS-C354082
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of SP1 expression in K562 (A); A431 (B) whole cell lysates.

Immunofluorescent analysis of SP1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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SP1 Antibody (phospho-Thr453) LS-C358335
LifeSpan Biosciences
catalog: LS-C358335
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of SP1 (pT453) expression in Jurkat (A); mouse melanoma cancer (B) whole cell lysates.

Immunohistochemical analysis of SP1 (pT453) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of SP1 (pT453) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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SP1 Antibody (C-Terminus) LS-C358868
LifeSpan Biosciences
catalog: LS-C358868
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of SP1 (pT739) expression in A549 (A); K562 (B); rat muscle (C) whole cell lysates.

Immunohistochemical analysis of SP1 (pT739) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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SP1 Antibody (390-470 aa) LS-C380845
LifeSpan Biosciences
catalog: LS-C380845
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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SP1 Antibody (680-760 aa) LS-C380846
LifeSpan Biosciences
catalog: LS-C380846
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-Sp1 (T739) antibody
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SP1 Antibody (390-470 aa) LS-C386148
LifeSpan Biosciences
catalog: LS-C386148
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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SP1 Antibody (phospho-Thr453) LS-C416503
LifeSpan Biosciences
catalog: LS-C416503
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of SP1 phosphorylation expression in A549 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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