LifeSpan Biosciences Sox9 antibody

PathPlus™ SOX9 Antibody (clone 3F11) LS-B4421
LifeSpan Biosciences
catalog: LS-B4421

mouse monoclonal (3F11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

SOX9 monoclonal antibody clone 3F11 Western blot of SOX9 expression in HepG2.

Immunofluorescence of monoclonal antibody to SOX9 on HepG2 cell (antibody concentration 10 ug/ml).

Anti-SOX9 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ SOX9 Antibody (aa147-196) LS-B5761
LifeSpan Biosciences
catalog: LS-B5761

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-SOX9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of A549 cells, using SOX9 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with PBS 60', using SOX9 Antibody. The lane on the right is blocked with the synthesized peptide.
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SOX9 Antibody (Internal) LS-C368527
LifeSpan Biosciences
catalog: LS-C368527

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 1

Western blot analysis of SOX9 expression in HepG2 (A); HeLa (B); NIH3T3 (C) whole cell lysates.

Immunohistochemical analysis of SOX9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of SOX9 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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SOX9 Antibody (aa34-83) LS-C31208
LifeSpan Biosciences
catalog: LS-C31208

domestic rabbit polyclonal
reactivity: human
application: western blot

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SOX9 Antibody (aa317-332) LS-C87217
LifeSpan Biosciences
catalog: LS-C87217

domestic goat polyclonal
reactivity: human, zebrafish

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SOX9 Antibody (aa308-320) LS-C87218
LifeSpan Biosciences
catalog: LS-C87218

domestic goat polyclonal
reactivity: human, zebrafish

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SOX9 Antibody (aa41-70) LS-C98107
LifeSpan Biosciences
catalog: LS-C98107

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of SOX9 (arrow) using rabbit polyclonal SOX9 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX9 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with SOX9 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence of anti-SOX9 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
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SOX9 Antibody (aa231-260) LS-C98108
LifeSpan Biosciences
catalog: LS-C98108

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Western blot of anti-SOX9 Antibody (RB13405) in HepG2 cell line lysates (35 ug/lane). SOX9(arrow) was detected using the purified antibody.

Flow cytometric of HepG2 cells using SOX9 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IHC-plus™ SOX9 Antibody (clone 3C10) LS-B4420
LifeSpan Biosciences
catalog: LS-B4420

mouse monoclonal (3C10)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

SOX9 monoclonal antibody clone 3C10 Western blot of SOX9 expression in HepG2.

Immunofluorescence of monoclonal antibody to SOX9 on HepG2 cell (antibody concentration 10 ug/ml).

SOX9 Antibody (C-Terminus) LS-C144931
LifeSpan Biosciences
catalog: LS-C144931

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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SOX9 Antibody (aa41-70) LS-C161405
LifeSpan Biosciences
catalog: LS-C161405

mouse monoclonal
reactivity: human
application: western blot

SOX9 Antibody western blot of K562 cell line lysates (35 ug/lane). The SOX9 antibody detected the SOX9 protein (arrow).
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SOX9 Antibody (aa41-70) LS-C161406
LifeSpan Biosciences
catalog: LS-C161406

mouse monoclonal
reactivity: human
application: western blot

SOX9 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The SOX9 antibody detected the SOX9 protein (arrow).
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SOX9 Antibody (clone 1B11) LS-C169454
LifeSpan Biosciences
catalog: LS-C169454

mouse monoclonal (1B11)
reactivity: human
application: western blot, ELISA, immunocytochemistry

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SOX9 Antibody (Gln175) LS-C176471
LifeSpan Biosciences
catalog: LS-C176471

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of SOX-9 (Q175) pAb in extracts from 293 cells treated with PBS 60'.

IHC of SOX-9 (Q175) pAb in paraffin-embedded human breast carcinoma tissue.
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SOX9 Antibody (phospho-Ser181) LS-C189206
LifeSpan Biosciences
catalog: LS-C189206

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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SOX9 Antibody (phospho-Ser181) LS-C199596
LifeSpan Biosciences
catalog: LS-C199596

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of A549 cells, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with PBS 60', using SOX-9 (Phospho-Ser181) Antibody. The lane on the right is blocked with the phospho peptide.
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SOX9 Antibody (aa231-260, AP) LS-C270031
LifeSpan Biosciences
catalog: LS-C270031

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, flow cytometry

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SOX9 Antibody (aa231-260, APC) LS-C270032
LifeSpan Biosciences
catalog: LS-C270032

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, flow cytometry

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SOX9 Antibody (aa231-260, Biotin) LS-C270033
LifeSpan Biosciences
catalog: LS-C270033

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, flow cytometry

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SOX9 Antibody (aa231-260, FITC) LS-C270034
LifeSpan Biosciences
catalog: LS-C270034

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, flow cytometry

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SOX9 Antibody (aa231-260, Azide-free, HRP) LS-C270035
LifeSpan Biosciences
catalog: LS-C270035

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, flow cytometry

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SOX9 Antibody (aa231-260, PE) LS-C270036
LifeSpan Biosciences
catalog: LS-C270036

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, flow cytometry

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SOX9 Antibody (aa41-70, AP) LS-C270037
LifeSpan Biosciences
catalog: LS-C270037

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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SOX9 Antibody (aa41-70, APC) LS-C270038
LifeSpan Biosciences
catalog: LS-C270038

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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SOX9 Antibody (aa41-70, Biotin) LS-C270039
LifeSpan Biosciences
catalog: LS-C270039

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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SOX9 Antibody (aa41-70, FITC) LS-C270040
LifeSpan Biosciences
catalog: LS-C270040

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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SOX9 Antibody (aa41-70, Azide-free, HRP) LS-C270041
LifeSpan Biosciences
catalog: LS-C270041

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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SOX9 Antibody (aa41-70, PE) LS-C270042
LifeSpan Biosciences
catalog: LS-C270042

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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SOX9 Antibody (phospho-Ser181) LS-C313636
LifeSpan Biosciences
catalog: LS-C313636

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry

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SOX9 Antibody (aa231-260) LS-C325292
LifeSpan Biosciences
catalog: LS-C325292

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, flow cytometry

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SOX9 Antibody (aa41-70) LS-C325294
LifeSpan Biosciences
catalog: LS-C325294

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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SOX9 Antibody (aa1-205, clone OTI3G3) LS-C336541
LifeSpan Biosciences
catalog: LS-C336541

mouse monoclonal (OTI3G3)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX9.
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SOX9 Antibody (aa1-205, clone OTI5G4) LS-C336582
LifeSpan Biosciences
catalog: LS-C336582

mouse monoclonal (OTI5G4)
reactivity: human
application: western blot

SOX9 Antibody (aa1-205, clone OTI2H10) LS-C338555
LifeSpan Biosciences
catalog: LS-C338555

mouse monoclonal (OTI2H10)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min).
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IHC-plus™ SOX9 Antibody (aa225-275) LS-B12075
LifeSpan Biosciences
catalog: LS-B12075

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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SOX9 Antibody (phospho-Ser181) LS-C352869
LifeSpan Biosciences
catalog: LS-C352869

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of SOX9 (pS181) expression in HeLa PMA-treated (A); SW480 (B); mouse brain (C); rat liver (D) whole cell lysates.
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SOX9 Antibody (120-200 aa) LS-C381483
LifeSpan Biosciences
catalog: LS-C381483

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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SOX9 Antibody (120-200 aa) LS-C386144
LifeSpan Biosciences
catalog: LS-C386144

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Sox-9 antibody
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