ST3GAL4 / ST3Gal IV Antibody (aa251-300) LS-C102240
LifeSpan Biosciences
catalog: LS-C102240
domestic rabbit polyclonal
reactivity: human
application: western blot
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ST3GAL4 / ST3Gal IV Antibody (clone 1F4) LS-C198197
LifeSpan Biosciences
catalog: LS-C198197
mouse monoclonal (1F4)
reactivity: human
application: western blot, ELISA

ST3GAL4 monoclonal antibody (M01), clone 1F4. Western Blot analysis of ST3GAL4 expression in MCF-7.

Detection limit for recombinant GST tagged ST3GAL4 is 3 ng/ml as a capture antibody.
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ST3GAL4 / ST3Gal IV Antibody LS-C335650
LifeSpan Biosciences
catalog: LS-C335650
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded rat kidney tissue.

Immunofluorescence analysis of HeLa cells.
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IHC-plus™ ST3GAL4 / ST3Gal IV Antibody (aa171-220) LS-B12621
LifeSpan Biosciences
catalog: LS-B12621
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.


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ST3GAL4 / ST3Gal IV Antibody LS-C482564
LifeSpan Biosciences
catalog: LS-C482564
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of ST3GAL4 expression in HT29 (A); rat testis (B); rat liver (C) whole cell lysates.

Immunohistochemical analysis of ST3GAL4 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of ST3GAL4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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