IHC-plus™ BMI1 / PCGF4 Antibody LS-B346
LifeSpan Biosciences
catalog: LS-B346

domestic goat polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-BMI-1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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BMI1 / PCGF4 Antibody (aa1-202) LS-C4191
LifeSpan Biosciences
catalog: LS-C4191

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

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BMI1 / PCGF4 Antibody LS-C19577
LifeSpan Biosciences
catalog: LS-C19577

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of BMI-1 in K562 cell lysate with BMI-1 Antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

Immunocytochemistry of BMI-1 in K562 cells with BMI-1 Antibody at 10 ?/ml.

Immunofluorescence of BMI-1 in K562 cells with BMI-1 antibody at 20 ug/ml.
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BMI1 / PCGF4 Antibody (aa257-286) LS-C98479
LifeSpan Biosciences
catalog: LS-C98479

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

Western blot of BMI1 Antibody antibody in 293 cell line lysates (35 ug/lane). BMI1(arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with BMI1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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BMI1 / PCGF4 Antibody LS-C4192
LifeSpan Biosciences
catalog: LS-C4192

mouse monoclonal
reactivity: human
application: western blot, ELISA

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BMI1 / PCGF4 Antibody LS-C4194
LifeSpan Biosciences
catalog: LS-C4194

domestic rabbit polyclonal
reactivity: human
application: western blot

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BMI1 / PCGF4 Antibody (aa1-100) LS-C49129
LifeSpan Biosciences
catalog: LS-C49129

domestic rabbit polyclonal
reactivity: human
application: western blot

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BMI1 / PCGF4 Antibody LS-C77105
LifeSpan Biosciences
catalog: LS-C77105

mouse monoclonal
reactivity: human
application: western blot

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BMI1 / PCGF4 Antibody (C-Terminus) LS-C30108
LifeSpan Biosciences
catalog: LS-C30108

domestic rabbit polyclonal
reactivity: human
application: western blot

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BMI1 / PCGF4 Antibody (Internal, Biotin) LS-C141367
LifeSpan Biosciences
catalog: LS-C141367

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

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BMI1 / PCGF4 Antibody (Internal, DY650) LS-C141368
LifeSpan Biosciences
catalog: LS-C141368

domestic rabbit polyclonal
reactivity: human
application: western blot

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BMI1 / PCGF4 Antibody (Internal, DY488) LS-C141369
LifeSpan Biosciences
catalog: LS-C141369

domestic rabbit polyclonal
reactivity: human
application: western blot

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BMI1 / PCGF4 Antibody (Internal, DY550) LS-C141371
LifeSpan Biosciences
catalog: LS-C141371

domestic rabbit polyclonal
reactivity: human
application: western blot

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BMI1 / PCGF4 Antibody (aa181-230) LS-C146455
LifeSpan Biosciences
catalog: LS-C146455

domestic rabbit polyclonal
reactivity: human
application: western blot

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BMI1 / PCGF4 Antibody LS-C147042
LifeSpan Biosciences
catalog: LS-C147042

domestic rabbit polyclonal
reactivity: human
application: western blot

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BMI1 / PCGF4 Antibody (aa270-320) LS-C148527
LifeSpan Biosciences
catalog: LS-C148527

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ BMI1 / PCGF4 Antibody (Internal, clone EPR3745(2)) LS-B8010
LifeSpan Biosciences
catalog: LS-B8010

domestic rabbit monoclonal (EPR3745(2))
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-BMI-1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
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BMI1 / PCGF4 Antibody LS-C156830
LifeSpan Biosciences
catalog: LS-C156830

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of BMI1 Antibody in MCF7 cell line lysates (35 ug/lane). BMI1 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with BMI1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescence of BMI1 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
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BMI1 / PCGF4 Antibody LS-C156831
LifeSpan Biosciences
catalog: LS-C156831

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry

BMI1 Antibody western blot of K562 cell line lysates (35 ug/lane). The BMI1 antibody detected the BMI1 protein (arrow).

Confocal immunofluorescence of BMI1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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BMI1 / PCGF4 Antibody (clone 3E3) LS-C169434
LifeSpan Biosciences
catalog: LS-C169434

mouse monoclonal (3E3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

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BMI1 / PCGF4 Antibody (clone LLBmi1-1) LS-C180218
LifeSpan Biosciences
catalog: LS-C180218

mouse monoclonal (LLBmi1-1)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

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BMI1 / PCGF4 Antibody (clone LLBmi1-1, Biotin) LS-C181005
LifeSpan Biosciences
catalog: LS-C181005

mouse monoclonal (LLBmi1-1)
reactivity: human
conjugate: biotin
application: western blot, immunocytochemistry, immunoprecipitation

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BMI1 / PCGF4 Antibody (clone LLBmi1-1, DY650) LS-C181006
LifeSpan Biosciences
catalog: LS-C181006

mouse monoclonal (LLBmi1-1)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

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BMI1 / PCGF4 Antibody (clone LLBmi1-1, DY488) LS-C181007
LifeSpan Biosciences
catalog: LS-C181007

mouse monoclonal (LLBmi1-1)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

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BMI1 / PCGF4 Antibody (clone LLBmi1-1, DY550) LS-C181009
LifeSpan Biosciences
catalog: LS-C181009

mouse monoclonal (LLBmi1-1)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

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BMI1 / PCGF4 Antibody (clone 2704C2a) LS-C184479
LifeSpan Biosciences
catalog: LS-C184479

mouse monoclonal (2704C2a)
reactivity: human

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BMI1 / PCGF4 Antibody (clone 4E10-1C5) LS-C196758
LifeSpan Biosciences
catalog: LS-C196758

mouse monoclonal (4E10-1C5)
reactivity: human
application: western blot, ELISA

Western Blot analysis of PCGF4 expression in transfected 293T cell line by PCGF4 monoclonal antibody (M02), clone 4E10-1C5.Lane 1: PCGF4 transfected lysate(36.9 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged BMI1 is 1 ng/ml as a capture antibody.
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BMI1 / PCGF4 Antibody LS-C211559
LifeSpan Biosciences
catalog: LS-C211559

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (HRP) LS-C211561
LifeSpan Biosciences
catalog: LS-C211561

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (FITC) LS-C211562
LifeSpan Biosciences
catalog: LS-C211562

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (Biotin) LS-C211563
LifeSpan Biosciences
catalog: LS-C211563

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (clone 282CT3.7.6, AP) LS-C221255
LifeSpan Biosciences
catalog: LS-C221255

mouse monoclonal (282CT3.7.6)
reactivity: human
conjugate: AP
application: western blot, ELISA, immunocytochemistry

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BMI1 / PCGF4 Antibody (clone 282CT3.7.6, APC) LS-C221257
LifeSpan Biosciences
catalog: LS-C221257

mouse monoclonal (282CT3.7.6)
reactivity: human
conjugate: APC
application: western blot, ELISA, immunocytochemistry

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BMI1 / PCGF4 Antibody (clone 282CT3.7.6, FITC) LS-C221258
LifeSpan Biosciences
catalog: LS-C221258

mouse monoclonal (282CT3.7.6)
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunocytochemistry

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BMI1 / PCGF4 Antibody (clone 282CT3.7.6, PE) LS-C221259
LifeSpan Biosciences
catalog: LS-C221259

mouse monoclonal (282CT3.7.6)
reactivity: human
conjugate: PE
application: western blot, ELISA, immunocytochemistry

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BMI1 / PCGF4 Antibody (Internal, AP) LS-C221537
LifeSpan Biosciences
catalog: LS-C221537

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (Internal, APC) LS-C221538
LifeSpan Biosciences
catalog: LS-C221538

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (Internal, FITC) LS-C221539
LifeSpan Biosciences
catalog: LS-C221539

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (Internal, PE) LS-C221541
LifeSpan Biosciences
catalog: LS-C221541

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (aa257-286, AP) LS-C224910
LifeSpan Biosciences
catalog: LS-C224910

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (aa257-286, APC) LS-C224911
LifeSpan Biosciences
catalog: LS-C224911

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (aa257-286, FITC) LS-C224912
LifeSpan Biosciences
catalog: LS-C224912

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (aa257-286, PE) LS-C224913
LifeSpan Biosciences
catalog: LS-C224913

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (AP) LS-C224914
LifeSpan Biosciences
catalog: LS-C224914

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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BMI1 / PCGF4 Antibody (APC) LS-C224915
LifeSpan Biosciences
catalog: LS-C224915

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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BMI1 / PCGF4 Antibody (FITC) LS-C224916
LifeSpan Biosciences
catalog: LS-C224916

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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BMI1 / PCGF4 Antibody (PE) LS-C224918
LifeSpan Biosciences
catalog: LS-C224918

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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BMI1 / PCGF4 Antibody (aa276-326) LS-C286819
LifeSpan Biosciences
catalog: LS-C286819

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

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BMI1 / PCGF4 Antibody (aa152-289) LS-C296000
LifeSpan Biosciences
catalog: LS-C296000

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Recombinant PCGF4, Human.

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BMI1 / PCGF4 Antibody (aa152-289, Biotin) LS-C298487
LifeSpan Biosciences
catalog: LS-C298487

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western Blot; Sample: Recombinant PCGF4, Human.

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BMI1 / PCGF4 Antibody LS-C305935
LifeSpan Biosciences
catalog: LS-C305935

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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BMI1 / PCGF4 Antibody LS-C311026
LifeSpan Biosciences
catalog: LS-C311026

domestic rabbit polyclonal
reactivity: human
application: western blot

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BMI1 / PCGF4 Antibody (aa136-152) LS-C312950
LifeSpan Biosciences
catalog: LS-C312950

domestic rabbit polyclonal
reactivity: human
application: western blot

WB of BMI1 / PCGF4 antibody. Lane 1: HELA Cell Lysate. Lane 2: HT1080 Cell Lysate. Lane 3: COLO320 Cell Lysate. Lane 4: MCF-7 Cell Lysate.
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BMI1 / PCGF4 Antibody LS-C192237
LifeSpan Biosciences
catalog: LS-C192237

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of BMI1 pAb in extracts from K562 cells.
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BMI1 / PCGF4 Antibody (Internal) LS-C319855
LifeSpan Biosciences
catalog: LS-C319855

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody LS-C326932
LifeSpan Biosciences
catalog: LS-C326932

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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BMI1 / PCGF4 Antibody (aa257-286) LS-C326933
LifeSpan Biosciences
catalog: LS-C326933

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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BMI1 / PCGF4 Antibody (clone 282CT3.7.6) LS-C329159
LifeSpan Biosciences
catalog: LS-C329159

mouse monoclonal (282CT3.7.6)
reactivity: human
application: western blot, ELISA, immunocytochemistry

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BMI1 / PCGF4 Antibody LS-C330834
LifeSpan Biosciences
catalog: LS-C330834

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines, using BMI1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunohistochemistry of paraffin-embedded human esophageal cancer using BMI1 antibody at dilution of 1:200 (x400 lens).

Immunofluorescence analysis of HeLa cell using BMI1 antibody. Blue: DAPI for nuclear staining.
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BMI1 / PCGF4 Antibody LS-C332018
LifeSpan Biosciences
catalog: LS-C332018

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of extracts of U-937 cell line, using BMI1 antibody.
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BMI1 / PCGF4 Antibody LS-C342007
LifeSpan Biosciences
catalog: LS-C342007

mouse monoclonal
reactivity: human
application: western blot

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BMI1 / PCGF4 Antibody (aa135-165) LS-C343996
LifeSpan Biosciences
catalog: LS-C343996

domestic rabbit polyclonal
reactivity: human
application: western blot

Bmi1 antibody Western blot. All lanes: Anti BMI1 at 0.5 ug/ml. Lane 1: Rat Spleen Tissue Lysate at 50 ug. Lane 2: HT1080 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 37 kD . Observed band size: 37 kD.
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BMI1 / PCGF4 Antibody (C-Terminus) LS-C347795
LifeSpan Biosciences
catalog: LS-C347795

domestic rabbit polyclonal
reactivity: human
application: ELISA

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BMI1 / PCGF4 Antibody (aa230-265) LS-C356650
LifeSpan Biosciences
catalog: LS-C356650

domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-BMI1 Antibody at 1:2000 dilution Lane 1: SW480 whole cell lysates Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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