SEMA4A / Semaphorin 4A Antibody (aa501-550) LS-C119263
LifeSpan Biosciences
catalog: LS-C119263

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COS7 and Jurkat cells, using SEMA4A Antibody. The lane on the right is blocked with the synthesized peptide.
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SEMA4A / Semaphorin 4A Antibody (aa238-287) LS-C31767
LifeSpan Biosciences
catalog: LS-C31767

domestic rabbit polyclonal
reactivity: human
application: western blot

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SEMA4A / Semaphorin 4A Antibody LS-C126255
LifeSpan Biosciences
catalog: LS-C126255

mouse polyclonal
reactivity: human
application: western blot, ELISA

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SEMA4A / Semaphorin 4A Antibody (aa151-180) LS-C168954
LifeSpan Biosciences
catalog: LS-C168954

domestic rabbit polyclonal
reactivity: human
application: western blot

SEMA4A Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The SEMA4A antibody detected the SEMA4A protein (arrow).
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SEMA4A / Semaphorin 4A Antibody (aa500-546) LS-C176949
LifeSpan Biosciences
catalog: LS-C176949

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of SEMA4A (N532) pAb in extracts from COS-7 cells.

IHC of SEMA4A (N532) pAb in paraffin-embedded human brain tissue.
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SEMA4A / Semaphorin 4A Antibody (clone 5E3) LS-C179410
LifeSpan Biosciences
catalog: LS-C179410

mouse monoclonal (5E3)
reactivity: human, mouse
application: immunoprecipitation, flow cytometry

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SEMA4A / Semaphorin 4A Antibody (aa151-180, Biotin) LS-C240545
LifeSpan Biosciences
catalog: LS-C240545

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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SEMA4A / Semaphorin 4A Antibody (aa151-180, APC) LS-C240546
LifeSpan Biosciences
catalog: LS-C240546

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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SEMA4A / Semaphorin 4A Antibody (aa151-180, AP) LS-C240547
LifeSpan Biosciences
catalog: LS-C240547

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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SEMA4A / Semaphorin 4A Antibody (aa151-180, FITC) LS-C240548
LifeSpan Biosciences
catalog: LS-C240548

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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SEMA4A / Semaphorin 4A Antibody (aa151-180, PE) LS-C240551
LifeSpan Biosciences
catalog: LS-C240551

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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SEMA4A / Semaphorin 4A Antibody (aa151-180, Azide-free, HRP) LS-C248110
LifeSpan Biosciences
catalog: LS-C248110

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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SEMA4A / Semaphorin 4A Antibody (aa151-180) LS-C324992
LifeSpan Biosciences
catalog: LS-C324992

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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SEMA4A / Semaphorin 4A Antibody (Internal) LS-C353523
LifeSpan Biosciences
catalog: LS-C353523

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of Semaphorin 4A expression in HeLa (A); COS7 (B) whole cell lysates.

Immunofluorescent analysis of Semaphorin 4A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation of Semaphorin 4A from 0.5mg Jurkat whole cell extract lysate using 5ug of Anti-Semaphorin 4A Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-Semaphorin 4A Antibody.
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SEMA4A / Semaphorin 4A Antibody (470-550 aa, Internal) LS-C386002
LifeSpan Biosciences
catalog: LS-C386002

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of SEMA4A antibody
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