MAP2K4 / MKK4 Antibody (phospho-Ser257/Thr261) LS-C16099
LifeSpan Biosciences
catalog: LS-C16099

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (clone 2D10D8, 4G11 B2) LS-C47190
LifeSpan Biosciences
catalog: LS-C47190

mouse monoclonal (2D10D8, 4G11 B2)
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (N-Terminus) LS-C16100
LifeSpan Biosciences
catalog: LS-C16100

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Ser80) LS-C16101
LifeSpan Biosciences
catalog: LS-C16101

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunoprecipitation

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Thr261) LS-C16098
LifeSpan Biosciences
catalog: LS-C16098

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Ser80) LS-C48459
LifeSpan Biosciences
catalog: LS-C48459

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Ser257) LS-C99484
LifeSpan Biosciences
catalog: LS-C99484

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of MAP2K4 (arrow) using rabbit polyclonal MAP2K4 Antibody (S257). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K4 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K4 Antibody (S257) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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MAP2K4 / MKK4 Antibody (phospho-Thr261) LS-C117475
LifeSpan Biosciences
catalog: LS-C117475

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SEK1/MKK4 (Phospho-Thr261) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with UV 15', using SEK1/MKK4 (Phospho-Thr261) Antibody. The lane on the right is blocked with the phospho peptide.
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MAP2K4 / MKK4 Antibody (aa223-272) LS-C117990
LifeSpan Biosciences
catalog: LS-C117990

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of A549 cells, using SEK1/MKK4/JNKK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SEK1/MKK4/JNKK1 Antibody. The picture on the right is blocked with the synthesized peptide.
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MAP2K4 / MKK4 Antibody (aa227-276) LS-C118244
LifeSpan Biosciences
catalog: LS-C118244

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SEK1/MKK4 Antibody. The picture on the right is blocked with the synthesized peptide.
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MAP2K4 / MKK4 Antibody (aa316-365) LS-C118898
LifeSpan Biosciences
catalog: LS-C118898

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from 293 andJurkat cells, using JNKK Antibody. The lane on the right is blocked with the synthesized peptide.
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MAP2K4 / MKK4 Antibody LS-C146998
LifeSpan Biosciences
catalog: LS-C146998

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa259-287) LS-C164445
LifeSpan Biosciences
catalog: LS-C164445

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Mouse Map2k4 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The Map2k4 antibody detected the Map2k4 protein (arrow).

Mouse Map2k4 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse cerebellum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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MAP2K4 / MKK4 Antibody (clone 5H4) LS-C169318
LifeSpan Biosciences
catalog: LS-C169318

mouse monoclonal (5H4)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (clone OTI4B4) LS-C114517
LifeSpan Biosciences
catalog: LS-C114517

mouse monoclonal (OTI4B4)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.

IHC of paraffin-embedded breast tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).

Anti-MAP2K4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4.
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price:
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MAP2K4 / MKK4 Antibody (clone OTI2E4) LS-C114518
LifeSpan Biosciences
catalog: LS-C114518

mouse monoclonal (OTI2E4)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.

IHC of paraffin-embedded breast tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).

Anti-MAP2K4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4.
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MAP2K4 / MKK4 Antibody (clone OTI1D6) LS-C114531
LifeSpan Biosciences
catalog: LS-C114531

mouse monoclonal (OTI1D6)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).

Anti-MAP2K4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4.
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price:
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MAP2K4 / MKK4 Antibody (Val255) LS-C176203
LifeSpan Biosciences
catalog: LS-C176203

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of SEK1/MKK4 (V255) pAb in paraffin-embedded human breast carcinoma tissue.
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MAP2K4 / MKK4 Antibody (Glu74) LS-C176204
LifeSpan Biosciences
catalog: LS-C176204

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of SEK1/MKK4 (E74) pAb in extracts from NIH/3T3 cells.
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MAP2K4 / MKK4 Antibody (Gln253) LS-C176557
LifeSpan Biosciences
catalog: LS-C176557

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

IHC of MEK-4 (Q253) pAb in paraffin-embedded human brain tissue.
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MAP2K4 / MKK4 Antibody (Lys350) LS-C176909
LifeSpan Biosciences
catalog: LS-C176909

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of SEK1 (K350) pAb in extracts from 293 cells.
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MAP2K4 / MKK4 Antibody (phospho-Ser80) LS-C177947
LifeSpan Biosciences
catalog: LS-C177947

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-SEK1/MKK4 (S80) pAb in extracts from NIH/3T3 cells.

IHC of p-SEK1/MKK4 (S80) pAb in paraffin-embedded human breast carcinoma tissue.
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MAP2K4 / MKK4 Antibody (phospho-Thr261) LS-C177948
LifeSpan Biosciences
catalog: LS-C177948

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

IHC of p-SEK1 /MKK4 (T261) pAb in paraffin-embedded human breast carcinoma tissue.
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MAP2K4 / MKK4 Antibody (phospho-Thr261) LS-C188821
LifeSpan Biosciences
catalog: LS-C188821

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Thr261) LS-C188822
LifeSpan Biosciences
catalog: LS-C188822

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Ser257) LS-C199559
LifeSpan Biosciences
catalog: LS-C199559

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain, using SEK1/MKK4/JNKK1 (Phospho-Ser257) Antibody. The picture on the right is blocked with the phospho peptide.
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MAP2K4 / MKK4 Antibody (aa222-271) LS-C205632
LifeSpan Biosciences
catalog: LS-C205632

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa259-287, AP) LS-C262524
LifeSpan Biosciences
catalog: LS-C262524

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa259-287, APC) LS-C262525
LifeSpan Biosciences
catalog: LS-C262525

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa259-287, Biotin) LS-C262526
LifeSpan Biosciences
catalog: LS-C262526

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa259-287, Azide-free, HRP) LS-C262527
LifeSpan Biosciences
catalog: LS-C262527

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa259-287, FITC) LS-C262528
LifeSpan Biosciences
catalog: LS-C262528

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa259-287, PE) LS-C262529
LifeSpan Biosciences
catalog: LS-C262529

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Ser257, AP) LS-C262530
LifeSpan Biosciences
catalog: LS-C262530

domestic rabbit polyclonal
reactivity: human
conjugate: AP
modification: phosphorylated
application: ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Ser257, APC) LS-C262531
LifeSpan Biosciences
catalog: LS-C262531

domestic rabbit polyclonal
reactivity: human
conjugate: APC
modification: phosphorylated
application: ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Ser257, Biotin) LS-C262532
LifeSpan Biosciences
catalog: LS-C262532

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
modification: phosphorylated
application: ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Ser257, FITC) LS-C262533
LifeSpan Biosciences
catalog: LS-C262533

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
modification: phosphorylated
application: ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Ser257, PE) LS-C262536
LifeSpan Biosciences
catalog: LS-C262536

domestic rabbit polyclonal
reactivity: human
conjugate: PE
modification: phosphorylated
application: ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Ser257, Azide-free, HRP) LS-C262537
LifeSpan Biosciences
catalog: LS-C262537

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
modification: phosphorylated
application: ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Thr261, AP) LS-C262989
LifeSpan Biosciences
catalog: LS-C262989

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Thr261, Biotin) LS-C262990
LifeSpan Biosciences
catalog: LS-C262990

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Thr261, APC) LS-C262991
LifeSpan Biosciences
catalog: LS-C262991

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Thr261, FITC) LS-C262992
LifeSpan Biosciences
catalog: LS-C262992

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Thr261, Azide-free, HRP) LS-C262993
LifeSpan Biosciences
catalog: LS-C262993

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Thr261, PE) LS-C263001
LifeSpan Biosciences
catalog: LS-C263001

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Ser257, APC) LS-C279152
LifeSpan Biosciences
catalog: LS-C279152

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Ser257, Biotin) LS-C279153
LifeSpan Biosciences
catalog: LS-C279153

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Ser257, AP) LS-C279154
LifeSpan Biosciences
catalog: LS-C279154

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Ser257, FITC) LS-C279155
LifeSpan Biosciences
catalog: LS-C279155

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Ser257, Azide-free, HRP) LS-C279156
LifeSpan Biosciences
catalog: LS-C279156

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Ser257, PE) LS-C279163
LifeSpan Biosciences
catalog: LS-C279163

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa50-100) LS-C289383
LifeSpan Biosciences
catalog: LS-C289383

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

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to the supplier

MAP2K4 / MKK4 Antibody (aa137-394) LS-C295392
LifeSpan Biosciences
catalog: LS-C295392

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant MAP2K4 / MKK4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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MAP2K4 / MKK4 Antibody (aa137-394, Biotin) LS-C298344
LifeSpan Biosciences
catalog: LS-C298344

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western blot of recombinant MAP2K4 / MKK4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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MAP2K4 / MKK4 Antibody LS-C308881
LifeSpan Biosciences
catalog: LS-C308881

mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody LS-C310925
LifeSpan Biosciences
catalog: LS-C310925

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa360-378) LS-C313373
LifeSpan Biosciences
catalog: LS-C313373

domestic rabbit polyclonal
reactivity: human
application: western blot

WB of MAP2K4 / MKK4 antibody. All lanes: Anti-MAP2K4 at 0.5ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: MM231 Whole Cell Lysate at 40ug. Lane 5: CEM Whole Cell Lysate at 40ug. Predicted bind size: 44KD. Observed bind size: 44KD.
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MAP2K4 / MKK4 Antibody (phospho-Ser257) LS-C322829
LifeSpan Biosciences
catalog: LS-C322829

domestic rabbit polyclonal
reactivity: human
application: ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa259-287) LS-C322830
LifeSpan Biosciences
catalog: LS-C322830

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Thr261) LS-C322964
LifeSpan Biosciences
catalog: LS-C322964

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (Ser257) LS-C329160
LifeSpan Biosciences
catalog: LS-C329160

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Ser80) LS-C336027
LifeSpan Biosciences
catalog: LS-C336027

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts from HepG2 cells.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.

Immunofluorescence staining of methanol-fixed Hela cells.
quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Thr261) LS-C336028
LifeSpan Biosciences
catalog: LS-C336028

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.

Immunofluorescence staining of methanol-fixed Hela cells.
quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (phospho-Thr261) LS-C352839
LifeSpan Biosciences
catalog: LS-C352839

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of MKK4 (pT261) expression in NIH3T3 PDGF-treated (A) whole cell lysates.

Immunohistochemical analysis of MKK4 (pT261) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of MKK4 (pT261) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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MAP2K4 / MKK4 Antibody (Internal) LS-C352842
LifeSpan Biosciences
catalog: LS-C352842

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of MKK4 expression in HeLa (A); HepG2 (B); K562 (C); A431 (D); L929 (E) whole cell lysates.
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MAP2K4 / MKK4 Antibody (phospho-Ser80) LS-C352843
LifeSpan Biosciences
catalog: LS-C352843

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of MKK4 (pS80) expression in HEK293 UV-treated (A) whole cell lysates.

Immunohistochemical analysis of MKK4 (pS80) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of MKK4 (pS80) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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price:
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MAP2K4 / MKK4 Antibody (Internal) LS-C368411
LifeSpan Biosciences
catalog: LS-C368411

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of MKK4 expression in HepG2 (A); K562 (B); HeLa (C) whole cell lysates.

Immunohistochemical analysis of MKK4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of MKK4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (C-Terminus) LS-C368842
LifeSpan Biosciences
catalog: LS-C368842

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of MKK4 expression in HeLa (A); HEK293T (B) whole cell lysates.
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MAP2K4 / MKK4 Antibody (aa2-397, FITC) LS-C369965
LifeSpan Biosciences
catalog: LS-C369965

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa2-397, HRP) LS-C369966
LifeSpan Biosciences
catalog: LS-C369966

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa2-397, Biotin) LS-C369967
LifeSpan Biosciences
catalog: LS-C369967

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (aa2-397) LS-C369968
LifeSpan Biosciences
catalog: LS-C369968

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (20-100 aa) LS-C380773
LifeSpan Biosciences
catalog: LS-C380773

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-MEK-4 (S80) antibody
quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (200-280 aa) LS-C380775
LifeSpan Biosciences
catalog: LS-C380775

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-MEK-4 (T261) antibody
quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (200-280 aa) LS-C381610
LifeSpan Biosciences
catalog: LS-C381610

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (200-280 aa) LS-C384503
LifeSpan Biosciences
catalog: LS-C384503

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (20-100 aa) LS-C384504
LifeSpan Biosciences
catalog: LS-C384504

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of MEK-4 antibody
quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (200-280 aa) LS-C384505
LifeSpan Biosciences
catalog: LS-C384505

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

quantity:
price:
to the supplier

MAP2K4 / MKK4 Antibody (290-370 aa, C-terminal) LS-C386021
LifeSpan Biosciences
catalog: LS-C386021

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of SEK1 antibody
quantity:
price:
to the supplier