IHC-plus™ IFIH1 / MDA5 Antibody LS-B2107
LifeSpan Biosciences
catalog: LS-B2107

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-MDA5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/ml.

Western blot of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.
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IHC-plus™ IFIH1 / MDA5 Antibody LS-B2349
LifeSpan Biosciences
catalog: LS-B2349

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-MDA5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/ml.

Western blot of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.
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IHC-plus™ IFIH1 / MDA5 Antibody (aa612-661) LS-B5622
LifeSpan Biosciences
catalog: LS-B5622

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-MDA5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IFIH1 / MDA5 Antibody (N-Terminus) LS-C55097
LifeSpan Biosciences
catalog: LS-C55097

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody (10 ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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IFIH1 / MDA5 Antibody (Internal Region) LS-C55098
LifeSpan Biosciences
catalog: LS-C55098

domestic goat polyclonal
reactivity: human, mouse

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IFIH1 / MDA5 Antibody (aa976-1025) LS-C119245
LifeSpan Biosciences
catalog: LS-C119245

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from Jurkat cells, using IFIH1 Antibody. The lane on the right is blocked with the synthesized peptide.
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IFIH1 / MDA5 Antibody (clone 3F6) LS-C134094
LifeSpan Biosciences
catalog: LS-C134094

mouse monoclonal (3F6)
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunoperoxidase of monoclonal antibody to IFIH1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Detection limit for recombinant GST tagged IFIH1 is 3 ng/ml as a capture antibody.
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IFIH1 / MDA5 Antibody (Arg470) LS-C191022
LifeSpan Biosciences
catalog: LS-C191022

domestic rabbit monoclonal
reactivity: human
application: western blot, immunoprecipitation

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IFIH1 / MDA5 Antibody LS-C308389
LifeSpan Biosciences
catalog: LS-C308389

mouse polyclonal
reactivity: human
application: western blot

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IFIH1 / MDA5 Antibody LS-C310700
LifeSpan Biosciences
catalog: LS-C310700

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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IFIH1 / MDA5 Antibody LS-C310701
LifeSpan Biosciences
catalog: LS-C310701

domestic rabbit polyclonal
reactivity: human
application: western blot

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IFIH1 / MDA5 Antibody LS-C193107
LifeSpan Biosciences
catalog: LS-C193107

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot.
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IFIH1 / MDA5 Antibody LS-C331984
LifeSpan Biosciences
catalog: LS-C331984

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of THP-1 cells.

Immunohistochemistry of paraffin-embedded human colon cancer tissue.

Immunofluorescence analysis of A549 cells.
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IFIH1 / MDA5 Antibody (clone OTI8C10) LS-C338827
LifeSpan Biosciences
catalog: LS-C338827

mouse monoclonal (OTI8C10)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIH1.
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IFIH1 / MDA5 Antibody (clone OTI11C11) LS-C338829
LifeSpan Biosciences
catalog: LS-C338829

mouse monoclonal (OTI11C11)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIH1.
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IFIH1 / MDA5 Antibody (clone OTI3E10) LS-C338830
LifeSpan Biosciences
catalog: LS-C338830

mouse monoclonal (OTI3E10)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIH1.
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IFIH1 / MDA5 Antibody LS-C343697
LifeSpan Biosciences
catalog: LS-C343697

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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IFIH1 / MDA5 Antibody (clone 1B6) LS-C343749
LifeSpan Biosciences
catalog: LS-C343749

mouse monoclonal (1B6)
reactivity: human
application: western blot, ELISA

Western blot of MDA5: Lane 1: MW Marker; Lane 2: HeLa; Lane 3: A431; Lane 4: L929; Lane 5: 3T3
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IFIH1 / MDA5 Antibody (aa78-555, clone Hely-1) LS-C344935
LifeSpan Biosciences
catalog: LS-C344935

mouse monoclonal (Hely-1)
reactivity: human
application: western blot, ELISA, immunoprecipitation

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IFIH1 / MDA5 Antibody (C-Terminus) LS-C353529
LifeSpan Biosciences
catalog: LS-C353529

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of MDA5 expression in HepG2 (A); THP1 (B) whole cell lysates.

Immunohistochemical analysis of MDA5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of MDA5 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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IFIH1 / MDA5 Antibody (aa605-815) LS-C373763
LifeSpan Biosciences
catalog: LS-C373763

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of IFIH1 / MDA5 antibody.

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IFIH1 / MDA5 Antibody (aa1-205) LS-C379837
LifeSpan Biosciences
catalog: LS-C379837

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human pancreas using antibody at 1:100 dilution.
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IFIH1 / MDA5 Antibody (940-1020 aa, C-terminal) LS-C384473
LifeSpan Biosciences
catalog: LS-C384473

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of MDA5 antibody
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