MAPK12 / ERK6 / SAPK3 Antibody (aa339-367) LS-C100751
LifeSpan Biosciences
catalog: LS-C100751
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of MAPK12 (arrow) using rabbit polyclonal hMAPK12-P354. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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MAPK12 / ERK6 / SAPK3 Antibody (clone 1G4) LS-C133423
LifeSpan Biosciences
catalog: LS-C133423
mouse monoclonal (1G4)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged MAPK12 is approximately 0.3 ng/ml as a capture antibody.
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MAPK12 / ERK6 / SAPK3 Antibody (aa336-367) LS-C99857
LifeSpan Biosciences
catalog: LS-C99857
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-P38gamma C-term antibody in mouse muscle tissue lysate. P38gamma (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with the P38 gamma antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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MAPK12 / ERK6 / SAPK3 Antibody (aa35-84) LS-C107534
LifeSpan Biosciences
catalog: LS-C107534
domestic rabbit polyclonal
reactivity: human
application: western blot
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MAPK12 / ERK6 / SAPK3 Antibody (aa289-338) LS-C135387
LifeSpan Biosciences
catalog: LS-C135387
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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MAPK12 / ERK6 / SAPK3 Antibody (clone OTI10E1) LS-C114599
LifeSpan Biosciences
catalog: LS-C114599
mouse monoclonal (OTI10E1)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK12.

IHC of paraffin-embedded breast tissue using anti-MAPK12 mouse monoclonal antibody. (Dilution 1:50).

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK12 antibody, and then analyzed by flow cytometry.
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MAPK12 / ERK6 / SAPK3 Antibody (clone OTI2E2) LS-C172494
LifeSpan Biosciences
catalog: LS-C172494
mouse monoclonal (OTI2E2)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPK7 monoclonal antibody.

Anti-MAPK7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK7 antibody, and then analyzed by flow cytometry.
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MAPK12 / ERK6 / SAPK3 Antibody (clone OTI3D4) LS-C172495
LifeSpan Biosciences
catalog: LS-C172495
mouse monoclonal (OTI3D4)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPK7 monoclonal antibody.

Anti-MAPK7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK7 antibody, and then analyzed by flow cytometry.
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MAPK12 / ERK6 / SAPK3 Antibody (clone OTI2F1) LS-C172516
LifeSpan Biosciences
catalog: LS-C172516
mouse monoclonal (OTI2F1)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK7.

Anti-MAPK7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK7 antibody, and then analyzed by flow cytometry.
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MAPK12 / ERK6 / SAPK3 Antibody (clone OTI4C1) LS-C172517
LifeSpan Biosciences
catalog: LS-C172517
mouse monoclonal (OTI4C1)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPK7 monoclonal antibody.

Anti-MAPK7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK7 antibody, and then analyzed by flow cytometry.
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MAPK12 / ERK6 / SAPK3 Antibody (clone OTI4D6) LS-C172518
LifeSpan Biosciences
catalog: LS-C172518
mouse monoclonal (OTI4D6)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPK7 monoclonal antibody.

Anti-MAPK7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK7 antibody, and then analyzed by flow cytometry.
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MAPK12 / ERK6 / SAPK3 Antibody (clone OTI5C7) LS-C172525
LifeSpan Biosciences
catalog: LS-C172525
mouse monoclonal (OTI5C7)
reactivity: human
application: western blot, immunocytochemistry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK7.

Anti-MAPK7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7.
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MAPK12 / ERK6 / SAPK3 Antibody (C-Terminus) LS-C188809
LifeSpan Biosciences
catalog: LS-C188809
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation
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MAPK12 / ERK6 / SAPK3 Antibody (aa314-363) LS-C206173
LifeSpan Biosciences
catalog: LS-C206173
domestic rabbit polyclonal
reactivity: human
application: western blot
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MAPK12 / ERK6 / SAPK3 Antibody (aa339-367, AP) LS-C219993
LifeSpan Biosciences
catalog: LS-C219993
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa339-367, APC) LS-C219994
LifeSpan Biosciences
catalog: LS-C219994
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa339-367, Biotin) LS-C219995
LifeSpan Biosciences
catalog: LS-C219995
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa339-367, FITC) LS-C219996
LifeSpan Biosciences
catalog: LS-C219996
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa339-367, Azide-free, HRP) LS-C219997
LifeSpan Biosciences
catalog: LS-C219997
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa339-367, PE) LS-C219998
LifeSpan Biosciences
catalog: LS-C219998
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa336-367, AP) LS-C219999
LifeSpan Biosciences
catalog: LS-C219999
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa336-367, Biotin) LS-C220000
LifeSpan Biosciences
catalog: LS-C220000
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa336-367, APC) LS-C220001
LifeSpan Biosciences
catalog: LS-C220001
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa336-367, FITC) LS-C220002
LifeSpan Biosciences
catalog: LS-C220002
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa336-367, Azide-free, HRP) LS-C220003
LifeSpan Biosciences
catalog: LS-C220003
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa336-367, PE) LS-C220004
LifeSpan Biosciences
catalog: LS-C220004
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (clone 4F4-3D2) LS-C308883
LifeSpan Biosciences
catalog: LS-C308883
mouse monoclonal (4F4-3D2)
reactivity: human
application: western blot, ELISA, immunocytochemistry
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MAPK12 / ERK6 / SAPK3 Antibody LS-C310934
LifeSpan Biosciences
catalog: LS-C310934
domestic rabbit polyclonal
reactivity: human
application: western blot
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MAPK12 / ERK6 / SAPK3 Antibody LS-C310935
LifeSpan Biosciences
catalog: LS-C310935
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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MAPK12 / ERK6 / SAPK3 Antibody LS-C314011
LifeSpan Biosciences
catalog: LS-C314011
domestic rabbit polyclonal
reactivity: human
application: western blot
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MAPK12 / ERK6 / SAPK3 Antibody (aa336-367) LS-C327831
LifeSpan Biosciences
catalog: LS-C327831
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody (aa339-367) LS-C330531
LifeSpan Biosciences
catalog: LS-C330531
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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MAPK12 / ERK6 / SAPK3 Antibody LS-C331007
LifeSpan Biosciences
catalog: LS-C331007
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of mouse heart, using MAPK12 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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MAPK12 / ERK6 / SAPK3 Antibody (aa319-353) LS-C344708
LifeSpan Biosciences
catalog: LS-C344708
domestic rabbit polyclonal (RB47232)
reactivity: human, zebrafish
application: western blot

Western blot of lysates from zebra fish brain, Zebrafish tissue lysate (from left to right) with (DANRE) mapk12 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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MAPK12 / ERK6 / SAPK3 Antibody (Internal) LS-C352817
LifeSpan Biosciences
catalog: LS-C352817
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of SAPK3 expression in Jurkat (A); HeLa (B); NIH3T3 (C); H9C2 (D) whole cell lysates.
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MAPK12 / ERK6 / SAPK3 Antibody (aa27-311) LS-C373169
LifeSpan Biosciences
catalog: LS-C373169
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of MAPK12 / ERK6 / SAPK3 antibody.

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