RPS6KB2 / S6K2 Antibody (aa452-482) LS-C99823
LifeSpan Biosciences
catalog: LS-C99823
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of anti-p70S6Kbeta antibody in A375 cell lysate. p70S6Kbeta (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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RPS6KB2 / S6K2 Antibody (aa243-272) LS-C99824
LifeSpan Biosciences
catalog: LS-C99824
domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot of RPS6KB2 (arrow) using rabbit polyclonal RPS6KB2 Antibody (RB11689). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human RPS6KB2 gene (Lane 2) (Origene Technologies).
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RPS6KB2 / S6K2 Antibody (aa389-438) LS-C118316
LifeSpan Biosciences
catalog: LS-C118316
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from 293 cells, using p70 S6 Kinase beta Antibody. The lane on the right is blocked with the synthesized peptide.
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RPS6KB2 / S6K2 Antibody LS-C162756
LifeSpan Biosciences
catalog: LS-C162756
mouse monoclonal
reactivity: human
application: western blot

RPS6KB2 Antibody western blot of Jurkat, MCF-7, mouse NIH/3T3 cell lysates (35 ug/lane). This demonstrates that the RPS6KB2 antibody detected RPS6KB2 protein (arrow).
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RPS6KB2 / S6K2 Antibody LS-C162757
LifeSpan Biosciences
catalog: LS-C162757
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of RPS6KB2 Antibody in K562 cell line lysates (35 ug/lane). RPS6KB2 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded mouse brain tissue reacted with RPS6KB2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

RPS6KB2 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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RPS6KB2 / S6K2 Antibody (aa318-346) LS-C164570
LifeSpan Biosciences
catalog: LS-C164570
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Mouse Rps6kb2 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The Rps6kb2 antibody detected the Rps6kb2 protein (arrow).
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RPS6KB2 / S6K2 Antibody (aa2-410) LS-C184865
LifeSpan Biosciences
catalog: LS-C184865
domestic goat polyclonal
reactivity: human
application: western blot
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RPS6KB2 / S6K2 Antibody (phospho-Ser423) LS-C199393
LifeSpan Biosciences
catalog: LS-C199393
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 5', using p70 S6 Kinase beta (Phospho-Ser423) Antibody. The lane on the right is blocked with the phospho peptide.
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RPS6KB2 / S6K2 Antibody (aa368-417) LS-C200454
LifeSpan Biosciences
catalog: LS-C200454
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HUVECcells using p70 S6 Kinase beta antibody.
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RPS6KB2 / S6K2 Antibody LS-C211555
LifeSpan Biosciences
catalog: LS-C211555
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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RPS6KB2 / S6K2 Antibody (HRP) LS-C211556
LifeSpan Biosciences
catalog: LS-C211556
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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RPS6KB2 / S6K2 Antibody (FITC) LS-C211557
LifeSpan Biosciences
catalog: LS-C211557
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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RPS6KB2 / S6K2 Antibody (Biotin) LS-C211558
LifeSpan Biosciences
catalog: LS-C211558
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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RPS6KB2 / S6K2 Antibody (clone 164CT21.2.2, AP) LS-C212525
LifeSpan Biosciences
catalog: LS-C212525
mouse monoclonal (164CT21.2.2)
reactivity: human
conjugate: AP
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (clone 164CT21.2.2, APC) LS-C212526
LifeSpan Biosciences
catalog: LS-C212526
mouse monoclonal (164CT21.2.2)
reactivity: human
conjugate: APC
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (clone 164CT21.2.2, Biotin) LS-C212527
LifeSpan Biosciences
catalog: LS-C212527
mouse monoclonal (164CT21.2.2)
reactivity: human
conjugate: biotin
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (clone 164CT21.2.2, Azide-free, HRP) LS-C212528
LifeSpan Biosciences
catalog: LS-C212528
mouse monoclonal (164CT21.2.2)
reactivity: human
conjugate: HRP
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (clone 164CT21.2.2, FITC) LS-C212529
LifeSpan Biosciences
catalog: LS-C212529
mouse monoclonal (164CT21.2.2)
reactivity: human
conjugate: FITC
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (clone 164CT21.2.2, PE) LS-C212530
LifeSpan Biosciences
catalog: LS-C212530
mouse monoclonal (164CT21.2.2)
reactivity: human
conjugate: PE
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (aa452-482, AP) LS-C216581
LifeSpan Biosciences
catalog: LS-C216581
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, flow cytometry
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RPS6KB2 / S6K2 Antibody (aa452-482, APC) LS-C216582
LifeSpan Biosciences
catalog: LS-C216582
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, flow cytometry
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RPS6KB2 / S6K2 Antibody (aa452-482, Biotin) LS-C216583
LifeSpan Biosciences
catalog: LS-C216583
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, flow cytometry
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RPS6KB2 / S6K2 Antibody (aa452-482, FITC) LS-C216584
LifeSpan Biosciences
catalog: LS-C216584
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, flow cytometry
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RPS6KB2 / S6K2 Antibody (aa452-482, PE) LS-C216586
LifeSpan Biosciences
catalog: LS-C216586
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, flow cytometry
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RPS6KB2 / S6K2 Antibody (aa452-482, Azide-free, HRP) LS-C216587
LifeSpan Biosciences
catalog: LS-C216587
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, flow cytometry
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RPS6KB2 / S6K2 Antibody (AP) LS-C217330
LifeSpan Biosciences
catalog: LS-C217330
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, flow cytometry
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RPS6KB2 / S6K2 Antibody (APC) LS-C217331
LifeSpan Biosciences
catalog: LS-C217331
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry
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RPS6KB2 / S6K2 Antibody (Biotin) LS-C217332
LifeSpan Biosciences
catalog: LS-C217332
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, flow cytometry
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RPS6KB2 / S6K2 Antibody (Azide-free, HRP) LS-C217333
LifeSpan Biosciences
catalog: LS-C217333
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, flow cytometry
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RPS6KB2 / S6K2 Antibody (FITC) LS-C217334
LifeSpan Biosciences
catalog: LS-C217334
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry
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RPS6KB2 / S6K2 Antibody (aa318-346, APC) LS-C217337
LifeSpan Biosciences
catalog: LS-C217337
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: APC
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (aa318-346, Biotin) LS-C217338
LifeSpan Biosciences
catalog: LS-C217338
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (aa318-346, FITC) LS-C217339
LifeSpan Biosciences
catalog: LS-C217339
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (aa318-346, Azide-free, HRP) LS-C217340
LifeSpan Biosciences
catalog: LS-C217340
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (aa318-346, PE) LS-C217344
LifeSpan Biosciences
catalog: LS-C217344
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: PE
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (aa430-C-term) LS-C287458
LifeSpan Biosciences
catalog: LS-C287458
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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RPS6KB2 / S6K2 Antibody (aa194-453) LS-C296289
LifeSpan Biosciences
catalog: LS-C296289
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant RPS6KB2 / S6K2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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IHC-plus™ RPS6KB2 / S6K2 Antibody (aa300-350) LS-B11303
LifeSpan Biosciences
catalog: LS-B11303
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-RPS6KB2 / S6K2 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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RPS6KB2 / S6K2 Antibody (clone 164CT21.2.2) LS-C319206
LifeSpan Biosciences
catalog: LS-C319206
mouse monoclonal (164CT21.2.2)
reactivity: human
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (aa452-482) LS-C323747
LifeSpan Biosciences
catalog: LS-C323747
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, flow cytometry
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RPS6KB2 / S6K2 Antibody LS-C324825
LifeSpan Biosciences
catalog: LS-C324825
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry
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RPS6KB2 / S6K2 Antibody (aa318-346) LS-C330084
LifeSpan Biosciences
catalog: LS-C330084
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody LS-C331525
LifeSpan Biosciences
catalog: LS-C331525
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of various cell lines, using RPS6KB2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
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RPS6KB2 / S6K2 Antibody (C-Terminus) LS-C345150
LifeSpan Biosciences
catalog: LS-C345150
domestic rabbit polyclonal
reactivity: human
application: western blot
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RPS6KB2 / S6K2 Antibody (phospho-Ser423) LS-C357280
LifeSpan Biosciences
catalog: LS-C357280
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot of extracts from K562 cells, treated with EGF (200ng/ml, 5mins), using p70 S6 Kinase beta (Phospho-Ser423) antibody.
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IHC-plus™ RPS6KB2 / S6K2 Antibody (clone 4B11) LS-B12852
LifeSpan Biosciences
catalog: LS-B12852
mouse monoclonal (4B11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western Blot analysis of RPS6KB2 expression in transfected 293T cell line by RPS6KB2 monoclonal antibody (M08), clone 4B11.Lane 1: RPS6KB2 transfected lysate(53.4 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to RPS6KB2 on HeLa cell . [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged RPS6KB2 is approximately 0.1 ng/ml as a capture antibody.
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RPS6KB2 / S6K2 Antibody (C-Terminus) LS-C368506
LifeSpan Biosciences
catalog: LS-C368506
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of S6K2 expression in Jurkat (A); K562 (B) whole cell lysates.

Immunohistochemical analysis of S6K2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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RPS6KB2 / S6K2 Antibody (360-440 aa) LS-C381021
LifeSpan Biosciences
catalog: LS-C381021
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
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RPS6KB2 / S6K2 Antibody (360-440 aa) LS-C385320
LifeSpan Biosciences
catalog: LS-C385320
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of p70 S6 kinase beta antibody
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