RORA / ROR Alpha Antibody (aa239-288) LS-C40524
LifeSpan Biosciences
catalog: LS-C40524
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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RORA / ROR Alpha Antibody (aa101-150) LS-C40522
LifeSpan Biosciences
catalog: LS-C40522
domestic rabbit polyclonal
reactivity: human
application: western blot
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RORA / ROR Alpha Antibody (aa50-99) LS-C31274
LifeSpan Biosciences
catalog: LS-C31274
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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RORA / ROR Alpha Antibody (aa201-250) LS-C40523
LifeSpan Biosciences
catalog: LS-C40523
domestic rabbit polyclonal
reactivity: human
application: western blot
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RORA / ROR Alpha Antibody (aa288-337) LS-C109966
LifeSpan Biosciences
catalog: LS-C109966
domestic rabbit polyclonal
reactivity: human
application: western blot
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RORA / ROR Alpha Antibody (aa36-85) LS-C111052
LifeSpan Biosciences
catalog: LS-C111052
domestic rabbit polyclonal
reactivity: human
application: western blot
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RORA / ROR Alpha Antibody (aa36-85) LS-C112353
LifeSpan Biosciences
catalog: LS-C112353
domestic rabbit polyclonal
reactivity: human
application: western blot
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RORA / ROR Alpha Antibody (aa220-270) LS-C148467
LifeSpan Biosciences
catalog: LS-C148467
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ RORA / ROR Alpha Antibody (aa36-85) LS-B6839
LifeSpan Biosciences
catalog: LS-B6839
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ROR Alpha antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC-plus™ RORA / ROR Alpha Antibody (aa6-55) LS-B7055
LifeSpan Biosciences
catalog: LS-B7055
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ROR Alpha antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot analysis of lysates from HeLa and COS7 cells, using RORA Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ RORA / ROR Alpha Antibody (aa80-120) LS-B7230
LifeSpan Biosciences
catalog: LS-B7230
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ROR Alpha antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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PathPlus™ RORA / ROR Alpha Antibody (Ligand-binding Domain) LS-A5539
LifeSpan Biosciences
catalog: LS-A5539
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-RORA / ROR Alpha antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.


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RORA / ROR Alpha Antibody (aa201-250) LS-C170125
LifeSpan Biosciences
catalog: LS-C170125
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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RORA / ROR Alpha Antibody (Gly41) LS-C176905
LifeSpan Biosciences
catalog: LS-C176905
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of ROR (G41) pAb in extracts from HeLa cells.

IHC of ROR (G41) pAb in paraffin-embedded human breast carcinoma tissue.
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IHC-plus™ RORA / ROR Alpha Antibody (Thr216) LS-B8962
LifeSpan Biosciences
catalog: LS-B8962
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of anti-RORA Antibody (T216) in A2058 cell line lysates (35 ug/lane). RORA (arrow) was detected using the purified antibody.

Fluorescent confocal image of HeLa cell stained with RORA Antibody (T216). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RORA primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RORA immunoreactivity is localized to Nucleus significantly.

Prostate, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
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RORA / ROR Alpha Antibody (aa293-523) LS-C191248
LifeSpan Biosciences
catalog: LS-C191248
domestic rabbit polyclonal
reactivity: human
application: ELISA
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RORA / ROR Alpha Antibody (clone 4E3) LS-C198100
LifeSpan Biosciences
catalog: LS-C198100
mouse monoclonal (4E3)
reactivity: human
application: ELISA

Detection limit for recombinant GST tagged RORA is approximately 0.3 ng/ml as a capture antibody.
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RORA / ROR Alpha Antibody (Thr216, AP) LS-C282024
LifeSpan Biosciences
catalog: LS-C282024
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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RORA / ROR Alpha Antibody (Thr216, APC) LS-C282028
LifeSpan Biosciences
catalog: LS-C282028
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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RORA / ROR Alpha Antibody (Thr216, Biotin) LS-C282029
LifeSpan Biosciences
catalog: LS-C282029
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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RORA / ROR Alpha Antibody (Thr216, Azide-free, HRP) LS-C282031
LifeSpan Biosciences
catalog: LS-C282031
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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RORA / ROR Alpha Antibody (Thr216, FITC) LS-C282032
LifeSpan Biosciences
catalog: LS-C282032
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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RORA / ROR Alpha Antibody (Thr216, PE) LS-C282033
LifeSpan Biosciences
catalog: LS-C282033
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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RORA / ROR Alpha Antibody (aa164-401) LS-C296183
LifeSpan Biosciences
catalog: LS-C296183
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Knockout Varification: Lane 1: Wild-type PC3 cell lysate; Lane 2: RORa knockout PC3 cell lysate; Predicted MW: 63kd Observed MW: 54kd Primary Ab: 1µg/ml Rabbit Anti-Human RORa Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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RORA / ROR Alpha Antibody (Thr216) LS-C330630
LifeSpan Biosciences
catalog: LS-C330630
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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RORA / ROR Alpha Antibody (aa98-345, clone OTI2C4) LS-C336806
LifeSpan Biosciences
catalog: LS-C336806
mouse monoclonal (OTI2C4)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RORA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RORA.
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RORA / ROR Alpha Antibody (aa98-345, clone OTI2E8) LS-C338678
LifeSpan Biosciences
catalog: LS-C338678
mouse monoclonal (OTI2E8)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RORA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RORA.
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RORA / ROR Alpha Antibody (N-Terminus) LS-C345875
LifeSpan Biosciences
catalog: LS-C345875
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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RORA / ROR Alpha Antibody LS-C346166
LifeSpan Biosciences
catalog: LS-C346166
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of extracts of H460 cells.

Immunofluorescence analysis of MCF7 cells.
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RORA / ROR Alpha Antibody (N-Terminus) LS-C368508
LifeSpan Biosciences
catalog: LS-C368508
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of ROR alpha expression in HeLa (A); HEK293T (B); HepG2 (C); U266 (D); OPM2 (E); PC12 (F) whole cell lysates.

Immunohistochemical analysis of ROR alpha staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of ROR alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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RORA / ROR Alpha Antibody (20-100 aa, N-terminal) LS-C385949
LifeSpan Biosciences
catalog: LS-C385949
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of RORalpha antibody
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