RET Antibody (aa896-1063, clone 6E4C4) LS-C41514
LifeSpan Biosciences
catalog: LS-C41514
mouse monoclonal (6E4C4)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity:
price:
to the supplier
RET Antibody (phospho-Tyr905) LS-C38815
LifeSpan Biosciences
catalog: LS-C38815
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunoprecipitation
quantity:
price:
to the supplier
RET Antibody (aa896-1063, clone 8D10C9) LS-C41512
LifeSpan Biosciences
catalog: LS-C41512
mouse monoclonal (8D10C9)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity:
price:
to the supplier
RET Antibody (APC) LS-C75423
LifeSpan Biosciences
catalog: LS-C75423
mouse monoclonal
reactivity: human
conjugate: APC
application: flow cytometry
quantity:
price:
to the supplier
RET Antibody LS-C75422
LifeSpan Biosciences
catalog: LS-C75422
mouse monoclonal
reactivity: human
application: western blot, ELISA, flow cytometry

Flow Cytometry Figure - Cell Count vs. Ret: SHSY-5Y human neuroblastoma cells were stained with Ret (filled histogram) or isotype control (open histogram) followed by APC-conjugated anti-mouse antibody.
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RET Antibody (Extracellular Domain, AP) LS-C87108
LifeSpan Biosciences
catalog: LS-C87108
domestic goat polyclonal
reactivity: human, mouse
conjugate: AP
application: immunocytochemistry
quantity:
price:
to the supplier
RET Antibody (aa1063-1093) LS-C99119
LifeSpan Biosciences
catalog: LS-C99119
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of Ret Antibody (C-term T1078) in SKBR3 cell lysate. Ret (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
quantity:
price:
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RET Antibody (aa13-44) LS-C99120
LifeSpan Biosciences
catalog: LS-C99120
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Western blot of hRET-G28 in MCF7 cell line lysates (35 ug/lane). RET (arrow) was detected using the purified antibody.

Flow cytometric of MCF-7 cells using RET Antibody (N-term Q28) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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RET Antibody (phospho-Tyr1062) LS-C117472
LifeSpan Biosciences
catalog: LS-C117472
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from K562 cells, using Ret (Phospho-Tyr1062) Antibody. The lane on the right is blocked with the phospho peptide.
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RET Antibody (aa1041-1090) LS-C118241
LifeSpan Biosciences
catalog: LS-C118241
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from K562 cells, using Ret Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
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IHC-plus™ RET Antibody (aa152-182) LS-B6584
LifeSpan Biosciences
catalog: LS-B6584
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-RET antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot of anti-Ret antibody in SKBR3 cell lysate. Ret (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
quantity:
price:
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PathPlus™ RET Antibody (aa1012-1042) LS-B6923
LifeSpan Biosciences
catalog: LS-B6923
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-RET antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot of RET (arrow) using rabbit polyclonal RET Antibody (C-term L1027). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RET gene (Lane 2) (Origene Technologies).
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RET Antibody (aa867-916) LS-C151180
LifeSpan Biosciences
catalog: LS-C151180
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
RET Antibody LS-C155981
LifeSpan Biosciences
catalog: LS-C155981
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of RET (arrow) using rabbit polyclonal RET Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RET gene.
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RET Antibody LS-C162547
LifeSpan Biosciences
catalog: LS-C162547
mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

RET western blot of A549 cell line lysates (35 ug/lane). The RET antibody detected the RET protein (arrow).

Confocal immunofluorescence of RET Antibody (Ascites) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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RET Antibody (phospho-Tyr1062) LS-C192207
LifeSpan Biosciences
catalog: LS-C192207
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa881-930) LS-C199525
LifeSpan Biosciences
catalog: LS-C199525
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HUVEC cells, using Ret Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using Ret Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using Ret Antibody. The lane on the right is blocked with the synthesized peptide.
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RET Antibody (phospho-Tyr1015) LS-C199526
LifeSpan Biosciences
catalog: LS-C199526
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using Ret (Phospho-Tyr1015) Antibody. The lane on the right is blocked with the phospho peptide.
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RET Antibody (aa1012-1042, AP) LS-C239917
LifeSpan Biosciences
catalog: LS-C239917
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa1012-1042, APC) LS-C239918
LifeSpan Biosciences
catalog: LS-C239918
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa1012-1042, Biotin) LS-C239919
LifeSpan Biosciences
catalog: LS-C239919
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa1012-1042, FITC) LS-C239920
LifeSpan Biosciences
catalog: LS-C239920
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa1012-1042, PE) LS-C239921
LifeSpan Biosciences
catalog: LS-C239921
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa1063-1093, AP) LS-C239922
LifeSpan Biosciences
catalog: LS-C239922
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
quantity:
price:
to the supplier
RET Antibody (aa1063-1093, APC) LS-C239923
LifeSpan Biosciences
catalog: LS-C239923
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
quantity:
price:
to the supplier
RET Antibody (aa1063-1093, Biotin) LS-C239924
LifeSpan Biosciences
catalog: LS-C239924
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
quantity:
price:
to the supplier
RET Antibody (aa1063-1093, FITC) LS-C239925
LifeSpan Biosciences
catalog: LS-C239925
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
quantity:
price:
to the supplier
RET Antibody (aa152-182, AP) LS-C239926
LifeSpan Biosciences
catalog: LS-C239926
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa152-182, APC) LS-C239927
LifeSpan Biosciences
catalog: LS-C239927
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa1063-1093, PE) LS-C239928
LifeSpan Biosciences
catalog: LS-C239928
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
quantity:
price:
to the supplier
RET Antibody (aa152-182, Biotin) LS-C239929
LifeSpan Biosciences
catalog: LS-C239929
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa152-182, FITC) LS-C239930
LifeSpan Biosciences
catalog: LS-C239930
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa152-182, PE) LS-C239931
LifeSpan Biosciences
catalog: LS-C239931
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa13-44, FITC) LS-C239935
LifeSpan Biosciences
catalog: LS-C239935
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity:
price:
to the supplier
RET Antibody (aa1012-1042, Azide-free, HRP) LS-C248648
LifeSpan Biosciences
catalog: LS-C248648
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa1063-1093, Azide-free, HRP) LS-C248649
LifeSpan Biosciences
catalog: LS-C248649
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
quantity:
price:
to the supplier
RET Antibody (aa152-182, Azide-free, HRP) LS-C248650
LifeSpan Biosciences
catalog: LS-C248650
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (phospho-Tyr905) LS-C291164
LifeSpan Biosciences
catalog: LS-C291164
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain, using Anti-RET Antibody (phospho-Tyr905). The picture on the right is blocked with the phospho peptide.
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PathPlus™ RET Antibody (clone 1A5) LS-B10954
LifeSpan Biosciences
catalog: LS-B10954
mouse monoclonal (1A5)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Detection limit for recombinant GST tagged RET is approximately 3 ng/ml as a capture antibody.

Anti-RET antibody IHC staining of human small intestine, myenteric plecus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
quantity:
price:
to the supplier
RET Antibody LS-C298762
LifeSpan Biosciences
catalog: LS-C298762
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
quantity:
price:
to the supplier
RET Antibody (Biotin) LS-C305702
LifeSpan Biosciences
catalog: LS-C305702
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, immunocytochemistry, immunoprecipitation
quantity:
price:
to the supplier
RET Antibody (FITC) LS-C305741
LifeSpan Biosciences
catalog: LS-C305741
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot
quantity:
price:
to the supplier
RET Antibody (aa1012-1042) LS-C324614
LifeSpan Biosciences
catalog: LS-C324614
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa1063-1093) LS-C324616
LifeSpan Biosciences
catalog: LS-C324616
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
price:
to the supplier
RET Antibody (aa152-182) LS-C324619
LifeSpan Biosciences
catalog: LS-C324619
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (aa13-44) LS-C324625
LifeSpan Biosciences
catalog: LS-C324625
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity:
price:
to the supplier
RET Antibody (aa713-1017, clone OTI2G6) LS-C339693
LifeSpan Biosciences
catalog: LS-C339693
mouse monoclonal (OTI2G6)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
quantity:
price:
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RET Antibody (aa713-1017, clone OTI5C7) LS-C339694
LifeSpan Biosciences
catalog: LS-C339694
mouse monoclonal (OTI5C7)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.

IHC of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
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to the supplier
RET Antibody (aa713-1017, clone OTI2B3) LS-C339696
LifeSpan Biosciences
catalog: LS-C339696
mouse monoclonal (OTI2B3)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
quantity:
price:
to the supplier
RET Antibody (aa713-1017, clone OTI4H3) LS-C339708
LifeSpan Biosciences
catalog: LS-C339708
mouse monoclonal (OTI4H3)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.

IHC of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
RET Antibody (aa713-1017, clone OTI5D4) LS-C339709
LifeSpan Biosciences
catalog: LS-C339709
mouse monoclonal (OTI5D4)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
RET Antibody (aa713-1017, clone OTI1G1) LS-C339712
LifeSpan Biosciences
catalog: LS-C339712
mouse monoclonal (OTI1G1)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.

IHC of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
RET Antibody (aa713-1017, clone OTI5C5) LS-C339719
LifeSpan Biosciences
catalog: LS-C339719
mouse monoclonal (OTI5C5)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.

IHC of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
RET Antibody (aa713-1017, clone OTI21H4) LS-C339731
LifeSpan Biosciences
catalog: LS-C339731
mouse monoclonal (OTI21H4)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.

IHC of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
RET Antibody (aa713-1017, clone OTI5F2) LS-C339755
LifeSpan Biosciences
catalog: LS-C339755
mouse monoclonal (OTI5F2)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.

IHC of paraffin-embedded RET positive Human lung carcinoma tissue using anti-RET mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
RET Antibody (aa713-1017, clone OTI20B8) LS-C339769
LifeSpan Biosciences
catalog: LS-C339769
mouse monoclonal (OTI20B8)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.

IHC of paraffin-embedded RET positive Human lung carcinoma tissue using anti-RET mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
RET Antibody (aa713-1017, clone OTI21H5) LS-C339799
LifeSpan Biosciences
catalog: LS-C339799
mouse monoclonal (OTI21H5)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.

IHC of paraffin-embedded RET positive Human lung carcinoma tissue using anti-RET mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
RET Antibody (Extracellular Domain, clone 421R25) LS-C355104
LifeSpan Biosciences
catalog: LS-C355104
mouse monoclonal (421R25)
reactivity: human
application: western blot
quantity:
price:
to the supplier
RET Antibody (C-Terminus) LS-C358277
LifeSpan Biosciences
catalog: LS-C358277
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of RET expression in K562 (A) whole cell lysates.

Immunohistochemical analysis of RET staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of RET staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
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RET Antibody (C-Terminus) LS-C358278
LifeSpan Biosciences
catalog: LS-C358278
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of RET expression in K562 (A); HeLa (B) whole cell lysates.

Immunohistochemical analysis of RET staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of RET staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
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RET Antibody (phospho-Y1062 C-Terminus ) LS-C358281
LifeSpan Biosciences
catalog: LS-C358281
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of RET (pY1062) expression in K562 (A); mouse brain (B) whole cell lysates.

Immunohistochemical analysis of RET (pY1062) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of RET (pY1062) staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier
RET Antibody (aa29-300) LS-C376494
LifeSpan Biosciences
catalog: LS-C376494
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: RET antibody at 6 ug/ml+HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 124 kDa. Observed band size: 124 kDa Immunohistochemistry.

Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
quantity:
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RET Antibody (960-1040 aa) LS-C380920
LifeSpan Biosciences
catalog: LS-C380920
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
quantity:
price:
to the supplier
RET Antibody (1010-1090 aa) LS-C381115
LifeSpan Biosciences
catalog: LS-C381115
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
RET Antibody (850-930 aa) LS-C381741
LifeSpan Biosciences
catalog: LS-C381741
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
RET Antibody (1010-1090 aa) LS-C385848
LifeSpan Biosciences
catalog: LS-C385848
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of Ret antibody
quantity:
price:
to the supplier
RET Antibody (850-930 aa) LS-C385853
LifeSpan Biosciences
catalog: LS-C385853
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Ret antibody
quantity:
price:
to the supplier