Requiem / DPF2 Antibody (N-Terminus) LS-C30935
LifeSpan Biosciences
catalog: LS-C30935

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ Requiem / DPF2 Antibody (clone 2F6) LS-B4402
LifeSpan Biosciences
catalog: LS-B4402

mouse monoclonal (2F6)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of DPF2 expression in transfected 293T cell line by DPF2 monoclonal antibody clone 2F6.

Immunofluorescence of monoclonal antibody to DPF2 on HeLa cell (antibody concentration 10 ug/ml).

Anti-DPF2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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Requiem / DPF2 Antibody (C-Terminus) LS-C101707
LifeSpan Biosciences
catalog: LS-C101707

domestic rabbit polyclonal
reactivity: human
application: western blot

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Requiem / DPF2 Antibody (aa215-264) LS-C109757
LifeSpan Biosciences
catalog: LS-C109757

domestic rabbit polyclonal
reactivity: human
application: western blot

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Requiem / DPF2 Antibody (aa179-228) LS-C109758
LifeSpan Biosciences
catalog: LS-C109758

domestic rabbit polyclonal
reactivity: human
application: western blot

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Requiem / DPF2 Antibody (aa151-200) LS-C119185
LifeSpan Biosciences
catalog: LS-C119185

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of A549 cells, using REQU Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HT-29 cells, using REQU Antibody. The lane on the right is blocked with the synthesized peptide.
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Requiem / DPF2 Antibody LS-C125484
LifeSpan Biosciences
catalog: LS-C125484

mouse monoclonal
reactivity: human
application: western blot

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IHC-plus™ Requiem / DPF2 Antibody LS-B7012
LifeSpan Biosciences
catalog: LS-B7012

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-DPF2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of REQUIEM in human kidney tissue with REQUIEM antibody at 20 ug/ml.

Western blot analysis of Requiem in mouse kidney tissue lysate with Requiem antibody at 1 ug/ml.
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Requiem / DPF2 Antibody LS-C167512
LifeSpan Biosciences
catalog: LS-C167512

mouse monoclonal
reactivity: human
application: western blot

DPF2 antibody western blot of MDA-MB435 cell line lysates (35 ug/lane). The DPF2 antibody detected the DPF2 protein (arrow).
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Requiem / DPF2 Antibody (clone 2283C1a) LS-C184696
LifeSpan Biosciences
catalog: LS-C184696

mouse monoclonal (2283C1a)
reactivity: human

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Requiem / DPF2 Antibody (Glu174) LS-C192051
LifeSpan Biosciences
catalog: LS-C192051

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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Requiem / DPF2 Antibody (aa125-175) LS-C290260
LifeSpan Biosciences
catalog: LS-C290260

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

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Requiem / DPF2 Antibody (aa341-391) LS-C290261
LifeSpan Biosciences
catalog: LS-C290261

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

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Requiem / DPF2 Antibody (clone 2283C1a) LS-C342727
LifeSpan Biosciences
catalog: LS-C342727

mouse monoclonal (2283C1a)
reactivity: human
application: western blot

Western blot analysis of immunized recombinant protein, using anti-DPF2 monoclonal antibody.
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Requiem / DPF2 Antibody (aa125-159) LS-C356504
LifeSpan Biosciences
catalog: LS-C356504

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

All lanes : Anti-Dpf2 Antibody at 1:2000 dilution Lane 1: HeLa whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: mouse spleen lysates Lane 4: mouse thymus lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Requiem / DPF2 Antibody (Internal) LS-C368676
LifeSpan Biosciences
catalog: LS-C368676

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of DPF2 expression in Jurkat (A); THP1 (B); K562 (C) whole cell lysates.

Immunohistochemical analysis of DPF2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of DPF2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Requiem / DPF2 Antibody (120-200 aa, Internal) LS-C383197
LifeSpan Biosciences
catalog: LS-C383197

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of DPF2 antibody
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