RAN Antibody (aa143-192) LS-C107810
LifeSpan Biosciences
catalog: LS-C107810

domestic rabbit polyclonal
reactivity: human
application: western blot

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RAN Antibody LS-C38721
LifeSpan Biosciences
catalog: LS-C38721

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

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RAN Antibody (Arg28/Asn143) LS-C93619
LifeSpan Biosciences
catalog: LS-C93619

domestic rabbit polyclonal
reactivity: human
application: western blot

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RAN Antibody LS-C109538
LifeSpan Biosciences
catalog: LS-C109538

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence of paraformaldehyde-fixed HeLa, using RAN antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate). A: Hela. 12% SDS PAGE. RAN antibody diluted at 1:1000.

Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
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RAN Antibody LS-C136853
LifeSpan Biosciences
catalog: LS-C136853

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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RAN Antibody (aa197-210) LS-C139726
LifeSpan Biosciences
catalog: LS-C139726

domestic goat polyclonal
reactivity: human
application: western blot

RAN antibody (1 ug/ml) staining of Human Testis lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC-plus™ RAN Antibody (aa167-216) LS-B7616
LifeSpan Biosciences
catalog: LS-B7616

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-RAN antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

Western blot analysis of lysates from LOVO cells, using RAN Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ RAN Antibody (aa2-14) LS-B8294
LifeSpan Biosciences
catalog: LS-B8294

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Staining (1 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Anti-RAN antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
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RAN Antibody (aa112-140) LS-C162426
LifeSpan Biosciences
catalog: LS-C162426

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

RAN Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The RAN antibody detected the RAN protein (arrow).

RAN antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

RAN Antibody flow cytometry of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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RAN Antibody (aa12-39) LS-C162428
LifeSpan Biosciences
catalog: LS-C162428

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.

Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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RAN Antibody (Glu202) LS-C176192
LifeSpan Biosciences
catalog: LS-C176192

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of RAN (E202) pAb in extracts from LOVO cells.

IHC of RAN (E202) pAb in paraffin-embedded human breast carcinoma tissue.
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RAN Antibody (clone ARAN1) LS-C179422
LifeSpan Biosciences
catalog: LS-C179422

mouse monoclonal (ARAN1)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

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RAN Antibody (aa112-140, AP) LS-C239742
LifeSpan Biosciences
catalog: LS-C239742

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, flow cytometry

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RAN Antibody (aa112-140, APC) LS-C239743
LifeSpan Biosciences
catalog: LS-C239743

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry

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RAN Antibody (aa112-140, Biotin) LS-C239744
LifeSpan Biosciences
catalog: LS-C239744

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, flow cytometry

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RAN Antibody (aa112-140, FITC) LS-C239745
LifeSpan Biosciences
catalog: LS-C239745

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry

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RAN Antibody (aa112-140, PE) LS-C239749
LifeSpan Biosciences
catalog: LS-C239749

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, flow cytometry

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RAN Antibody (aa112-140, Azide-free, HRP) LS-C248613
LifeSpan Biosciences
catalog: LS-C248613

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, flow cytometry

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RAN Antibody (aa175-225) LS-C290939
LifeSpan Biosciences
catalog: LS-C290939

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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RAN Antibody (aa166-216) LS-C290940
LifeSpan Biosciences
catalog: LS-C290940

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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RAN Antibody LS-C192300
LifeSpan Biosciences
catalog: LS-C192300

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of RAN pAb in extracts from NIH3T3, COS1, COS7, Jurkat, 293T cells and mouse testis tissue.
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RAN Antibody (aa112-140) LS-C324519
LifeSpan Biosciences
catalog: LS-C324519

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry

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RAN Antibody LS-C331176
LifeSpan Biosciences
catalog: LS-C331176

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Immunofluorescence analysis of MCF-7 cells.

Western blot analysis of extracts of various cell lines, using RAN antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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RAN Antibody (C-Terminus) LS-C352757
LifeSpan Biosciences
catalog: LS-C352757

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of RAN expression in HeLa (A); A431 (B) whole cell lysates.

Immunohistochemical analysis of RAN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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RAN Antibody LS-C356974
LifeSpan Biosciences
catalog: LS-C356974

domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-RAN Antibody at 1:8000 dilution Lane 1: HeLa whole cell lysates Lane 2: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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RAN Antibody (aa1-216) LS-C376438
LifeSpan Biosciences
catalog: LS-C376438

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: GTP-binding nuclear protein Ran antibody at 2 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: Jurkat whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 24 kDa. Observed band size: 24 kDa Immunohistochemistry.

Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
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RAN Antibody (140-220 aa, C-terminal) LS-C385773
LifeSpan Biosciences
catalog: LS-C385773

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Ran antibody
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