IHC-plus™ RAC1 Antibody LS-B6306
LifeSpan Biosciences
catalog: LS-B6306

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-RAC1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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RAC1 Antibody (phospho-Ser71) LS-C9854
LifeSpan Biosciences
catalog: LS-C9854

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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RAC1 Antibody LS-C137310
LifeSpan Biosciences
catalog: LS-C137310

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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RAC1 Antibody (phospho-Ser71) LS-C9853
LifeSpan Biosciences
catalog: LS-C9853

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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RAC1 Antibody (clone 23A8) LS-C85408
LifeSpan Biosciences
catalog: LS-C85408

mouse monoclonal (23A8)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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RAC1 Antibody (Ser71) LS-C100208
LifeSpan Biosciences
catalog: LS-C100208

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of RAC1 (arrow) using rabbit polyclonal RAC1 Antibody (S71). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RAC1 gene (Lane 2) (Origene Technologies).
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RAC1 Antibody (aa130-141) LS-C126205
LifeSpan Biosciences
catalog: LS-C126205

domestic rabbit polyclonal
reactivity: human
application: western blot

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RAC1 Antibody (aa1-196) LS-C185382
LifeSpan Biosciences
catalog: LS-C185382

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: Hela, B: Raji. 12% SDS PAGE. RAC1 antibody diluted at 1:1000.

IHC of paraffin-embedded H441 xenograft using RAC1 antibody at 1:100 dilution.
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RAC1 Antibody (Ser71, AP) LS-C281862
LifeSpan Biosciences
catalog: LS-C281862

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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RAC1 Antibody (Ser71, APC) LS-C281863
LifeSpan Biosciences
catalog: LS-C281863

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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RAC1 Antibody (Ser71, Biotin) LS-C281864
LifeSpan Biosciences
catalog: LS-C281864

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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RAC1 Antibody (Ser71, Azide-free, HRP) LS-C281865
LifeSpan Biosciences
catalog: LS-C281865

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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RAC1 Antibody (Ser71, FITC) LS-C281866
LifeSpan Biosciences
catalog: LS-C281866

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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RAC1 Antibody (Ser71, PE) LS-C281868
LifeSpan Biosciences
catalog: LS-C281868

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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RAC1 Antibody (Internal) LS-C118233
LifeSpan Biosciences
catalog: LS-C118233

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Rac1/CDC42 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, treated with EGF 200ng/ml 30', using Rac1/CDC42 Antibody. The lane on the right is blocked with the synthesized peptide.
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RAC1 Antibody (phospho-Ser71) LS-C192597
LifeSpan Biosciences
catalog: LS-C192597

domestic rabbit polyclonal
reactivity: human
application: western blot

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RAC1 Antibody (Ser71) LS-C328443
LifeSpan Biosciences
catalog: LS-C328443

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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IHC-plus™ RAC1 Antibody (C-Terminus) LS-B11533
LifeSpan Biosciences
catalog: LS-B11533

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAC1 Antibody LS-C334122
LifeSpan Biosciences
catalog: LS-C334122

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of extracts of 293 cell line, using RAC1 antibody.
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RAC1 Antibody (phospho-Ser71) LS-C335038
LifeSpan Biosciences
catalog: LS-C335038

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts from 293 cells.
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RAC1 Antibody (phospho-Ser71) LS-C335775
LifeSpan Biosciences
catalog: LS-C335775

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of a431 cells.
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RAC1 Antibody (clone 1301CT276.121.104) LS-C344161
LifeSpan Biosciences
catalog: LS-C344161

mouse monoclonal (1301CT276.121.104)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Flow cytometric of U-87 MG cells with RAC1 (green) compared to an isotype control of mouse IgG2b (blue). Antibody was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Immunohistochemical of paraffin-embedded H.skin section using RAC1. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot of lysates from A431, mouse NIH/3T3, rat C6 cell line (from left to right) with RAC1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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RAC1 Antibody (Internal) LS-C345609
LifeSpan Biosciences
catalog: LS-C345609

domestic rabbit polyclonal
reactivity: human
application: western blot

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RAC1 Antibody (aa1-189) LS-C373799
LifeSpan Biosciences
catalog: LS-C373799

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of RAC1 antibody.

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RAC1 Antibody LS-C482866
LifeSpan Biosciences
catalog: LS-C482866

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Rac 1 expression in BT474 (A); mouse liver (B); rat brain (C) whole cell lysates.

Immunohistochemical analysis of Rac 1 staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Rac 1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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