ARID1B / BAF250B Antibody (clone 2F2) LS-C133798
LifeSpan Biosciences
catalog: LS-C133798

mouse monoclonal (2f2)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

ARID1B monoclonal antibody (M02), clone 2F2 Western blot of ARID1B expression in HeLa NE.

Immunoperoxidase of monoclonal antibody to ARID1B on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Immunofluorescence of monoclonal antibody to ARID1B on HeLa cell. [antibody concentration 10 ug/ml]
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ARID1B / BAF250B Antibody (1340-1420 aa, Internal) LS-C382223
LifeSpan Biosciences
catalog: LS-C382223

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 1

Western blot of BAF250b antibody
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ARID1B / BAF250B Antibody (aa1371-1420) LS-C119154
LifeSpan Biosciences
catalog: LS-C119154

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of HUVEC cells, using BAF250B Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from LOVO cells, using BAF250B Antibody. The lane on the right is blocked with the synthesized peptide.
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ARID1B / BAF250B Antibody (aa1850-1900) LS-C286482
LifeSpan Biosciences
catalog: LS-C286482

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

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ARID1B / BAF250B Antibody (aa1300-1350) LS-C287875
LifeSpan Biosciences
catalog: LS-C287875

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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ARID1B / BAF250B Antibody (aa1850-1900) LS-C287877
LifeSpan Biosciences
catalog: LS-C287877

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunoprecipitation

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IHC-plus™ ARID1B / BAF250B Antibody (clone 2D2) LS-B10983
LifeSpan Biosciences
catalog: LS-B10983

mouse monoclonal (2D2)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

ARID1B monoclonal antibody (M01), clone 2D2 Western blot of ARID1B expression in HeLa NE.

Immunofluorescence of monoclonal antibody to ARID1B on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged ARID1B is approximately 0.03 ng/ml as a capture antibody.
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ARID1B / BAF250B Antibody (Internal) LS-C354185
LifeSpan Biosciences
catalog: LS-C354185

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of BAF250B expression in HeLa (A); HepG2 (B) whole cell lysates.

Immunohistochemical analysis of BAF250B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of BAF250B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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