IHC-plus™ PTHR / PTHR1 Antibody LS-B2267
LifeSpan Biosciences
catalog: LS-B2267

mouse monoclonal (3D1.1)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PTH1R antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ PTHR / PTHR1 Antibody (aa145-194) LS-B5322
LifeSpan Biosciences
catalog: LS-B5322

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-PTH1R antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Immunofluorescence analysis of MCF7 cells, using PTHR1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using PTHR1 Antibody. The lane on the right is blocked with the synthesized peptide.
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PTHR / PTHR1 Antibody (clone 3D11, Azide-free) LS-C87651
LifeSpan Biosciences
catalog: LS-C87651

mouse monoclonal (3D11)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed, paraffin-embedded human breast carcinoma stained with peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells. This image was taken for the unmodified form of this product. Other forms have not been tested.
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PTHR / PTHR1 Antibody (aa23-189) LS-C124346
LifeSpan Biosciences
catalog: LS-C124346

domestic sheep polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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IHC-plus™ PTHR / PTHR1 Antibody (N-Terminus) LS-A761
LifeSpan Biosciences
catalog: LS-A761

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-PTH1R antibody IHC of human kidney, renal tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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IHC-plus™ PTHR / PTHR1 Antibody (clone 3D1.1) LS-B7767
LifeSpan Biosciences
catalog: LS-B7767

mouse monoclonal (3D1.1)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PTHR / PTHR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
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PTHR / PTHR1 Antibody (clone 4D2) LS-C169236
LifeSpan Biosciences
catalog: LS-C169236

mouse monoclonal (4D2)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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PTHR / PTHR1 Antibody (N-Terminus) LS-C201397
LifeSpan Biosciences
catalog: LS-C201397

domestic rabbit polyclonal
reactivity: human
application: western blot

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PTHR / PTHR1 Antibody (aa388-406) LS-C313515
LifeSpan Biosciences
catalog: LS-C313515

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

WB of PTHR / PTHR1 antibody. Lane 1: SKOV Cell Lysate. Lane 2: U20S Cell Lysate. Lane 3: HELA Cell Lysate. Lane 4: SMMC Cell Lysate.

PTHR / PTHR1 antibody. IHC(P): Human Thyroid Cancer Tissue.
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PTHR / PTHR1 Antibody LS-C331666
LifeSpan Biosciences
catalog: LS-C331666

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using PTH1R antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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PTHR / PTHR1 Antibody (phospho-Thr1) LS-C358256
LifeSpan Biosciences
catalog: LS-C358256

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of PTHR1 expression in HEK293T (A); COLO205 (B) whole cell lysates.

Immunohistochemical analysis of PTHR1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PTHR1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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PTHR / PTHR1 Antibody (aa28-188) LS-C373014
LifeSpan Biosciences
catalog: LS-C373014

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Human Hela cell lysate; Primary Ab: 3µg/ml Rabbit Anti-Human PTHR1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PTHR / PTHR1 Antibody (120-200 aa, Internal) LS-C385666
LifeSpan Biosciences
catalog: LS-C385666

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of PTH/PTHrP-R antibody
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