LifeSpan Biosciences PTCH1 antibody

PTCH1 / Patched 1 Antibody (aa269-279) LS-C40590
LifeSpan Biosciences
catalog: LS-C40590

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

ptch1 Antibody (aa879-891) LS-C54781
LifeSpan Biosciences
catalog: LS-C54781

domestic goat polyclonal
reactivity: human, zebrafish
application: immunocytochemistry

IHC-plus™ PTCH1 / Patched 1 Antibody (aa1431-1442) LS-B3939
LifeSpan Biosciences
catalog: LS-B3939

domestic goat polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Anti-PTCH1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

PTCH1 / Patched 1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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PTCH1 / Patched 1 Antibody (clone OTI5C7) LS-C114391
LifeSpan Biosciences
catalog: LS-C114391

mouse monoclonal (OTI5C7)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1.

IHC of paraffin-embedded Adenocarcinoma of colon using anti-PTCH1 mouse monoclonal antibody.

Anti-PTCH1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTCH1.
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PTCH1 / Patched 1 Antibody (Pro1307) LS-C38104
LifeSpan Biosciences
catalog: LS-C38104

domestic rabbit monoclonal
reactivity: human
modification: phosphorylated
application: western blot, immunoprecipitation

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PTCH1 / Patched 1 Antibody (aa1271-1285) LS-C55339
LifeSpan Biosciences
catalog: LS-C55339

domestic goat polyclonal
reactivity: human
application: western blot, immunocytochemistry

PTCH1 / Patched 1 antibody (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

PTCH1 / Patched 1 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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PTCH1 / Patched 1 Antibody LS-C87098
LifeSpan Biosciences
catalog: LS-C87098

domestic rabbit polyclonal
reactivity: human
application: western blot

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PTCH1 / Patched 1 Antibody (aa269-279) LS-C93081
LifeSpan Biosciences
catalog: LS-C93081

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ PTCH1 / Patched 1 Antibody (aa58-107) LS-B5778
LifeSpan Biosciences
catalog: LS-B5778

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PTCH1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PTCH1 / Patched 1 Antibody (aa1200-1350) LS-C143235
LifeSpan Biosciences
catalog: LS-C143235

domestic rabbit polyclonal
reactivity: human
application: western blot

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PTCH1 / Patched 1 Antibody (N-Terminus) LS-C145062
LifeSpan Biosciences
catalog: LS-C145062

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry

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PTCH1 / Patched 1 Antibody (clone OTI3c2) LS-C114390
LifeSpan Biosciences
catalog: LS-C114390

mouse monoclonal (OTI3C2)
reactivity: human
application: western blot, immunocytochemistry

Anti-Patched1 mouse monoclonal antibody immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY PTCH1.
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PTCH1 / Patched 1 Antibody (Ala2) LS-C176173
LifeSpan Biosciences
catalog: LS-C176173

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of Patched (A2) pAb in extracts from mouse muscle cells.

IHC of Patched (A2) pAb in paraffin-embedded human brain tissue.
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PTCH1 / Patched 1 Antibody (Biotin) LS-C180635
LifeSpan Biosciences
catalog: LS-C180635

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

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PTCH1 / Patched 1 Antibody (DY650) LS-C180636
LifeSpan Biosciences
catalog: LS-C180636

domestic rabbit polyclonal
reactivity: human
application: western blot

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PTCH1 / Patched 1 Antibody (DY488) LS-C180637
LifeSpan Biosciences
catalog: LS-C180637

domestic rabbit polyclonal
reactivity: human
application: western blot

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PTCH1 / Patched 1 Antibody (DY550) LS-C180639
LifeSpan Biosciences
catalog: LS-C180639

domestic rabbit polyclonal
reactivity: human
application: western blot

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PTCH1 / Patched 1 Antibody (aa266-280) LS-C186591
LifeSpan Biosciences
catalog: LS-C186591

domestic goat polyclonal
reactivity: human
application: western blot, immunocytochemistry

PTCH1 antibody (2 ug/ml) staining of Mouse fetal Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PTCH1 / Patched 1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
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PTCH1 / Patched 1 Antibody (clone 1G2) LS-C198026
LifeSpan Biosciences
catalog: LS-C198026

mouse monoclonal (1G2)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged PTCH is approximately 0.03 ng/ml as a capture antibody.
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IHC-plus™ PTCH1 / Patched 1 Antibody (Cytoplasmic Domain) LS-B9983
LifeSpan Biosciences
catalog: LS-B9983

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PTCH1 / Patched antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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PTCH1 / Patched 1 Antibody (aa227-436) LS-C295773
LifeSpan Biosciences
catalog: LS-C295773

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant PTCH1 / Patched. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PTCH1 / Patched 1 Antibody (aa213-422) LS-C295775
LifeSpan Biosciences
catalog: LS-C295775

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant PTCH1 / Patched. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PTCH1 / Patched 1 Antibody (aa770-1027, clone OTI1H10) LS-C338857
LifeSpan Biosciences
catalog: LS-C338857

mouse monoclonal (OTI1H10)
reactivity: human
application: western blot

Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli.
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PTCH1 / Patched 1 Antibody (aa770-1027, clone OTI1H8) LS-C338871
LifeSpan Biosciences
catalog: LS-C338871

mouse monoclonal (OTI1H8)
reactivity: human
application: western blot

PTCH1 / Patched 1 Antibody (aa770-1027, clone OTI5G9) LS-C338892
LifeSpan Biosciences
catalog: LS-C338892

mouse monoclonal (OTI5G9)
reactivity: human
application: western blot

Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli.
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PTCH1 / Patched 1 Antibody (aa770-1027, clone OTI6C10) LS-C338939
LifeSpan Biosciences
catalog: LS-C338939

mouse monoclonal (OTI6C10)
reactivity: human
application: western blot

Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli.
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PTCH1 / Patched 1 Antibody LS-C356968
LifeSpan Biosciences
catalog: LS-C356968

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-Patched Antibody at 1:1000 dilution + MCF-7 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 161 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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PTCH1 / Patched 1 Antibody (aa1219-1447) LS-C357542
LifeSpan Biosciences
catalog: LS-C357542

domestic rabbit polyclonal
reactivity: human
application: western blot

Patched antibody Western blot. All lanes: Anti Patched at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: Mouse Brain Tissue Lysate at 50 ug. Lane 4: Mouse Liver Tissue Lysate at 50 ug. Predicted band size: 160 kD. Observed band size: 160 kD.
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PTCH1 / Patched 1 Antibody (1-80 aa, N-terminal) LS-C385375
LifeSpan Biosciences
catalog: LS-C385375

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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