IHC-plus™ SPHK2 Antibody (N-Terminus) LS-B1101
LifeSpan Biosciences
catalog: LS-B1101

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-SPHK2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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SPHK2 Antibody (C-Terminus) LS-C26797
LifeSpan Biosciences
catalog: LS-C26797

domestic rabbit polyclonal
reactivity: human
application: western blot

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SPHK2 Antibody (aa590-620) LS-C100772
LifeSpan Biosciences
catalog: LS-C100772

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

SPHK2 Antibody (A605) western blot of HepG2 cell line lysates (35 ug/lane). The SPHK2 antibody detected the SPHK2 protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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SPHK2 Antibody LS-C26940
LifeSpan Biosciences
catalog: LS-C26940

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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SPHK2 Antibody (aa580-629) LS-C118372
LifeSpan Biosciences
catalog: LS-C118372

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SPHK2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using SPHK2 Antibody. The lane on the right is blocked with the synthesized peptide.
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SPHK2 Antibody LS-C147009
LifeSpan Biosciences
catalog: LS-C147009

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

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SPHK2 Antibody (phospho-Thr614) LS-C199598
LifeSpan Biosciences
catalog: LS-C199598

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SPHK2 (Phospho-Thr614) Antibody. The picture on the right is blocked with the phospho peptide.
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SPHK2 Antibody (N-Terminus, AP) LS-C245951
LifeSpan Biosciences
catalog: LS-C245951

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (N-Terminus, APC) LS-C245952
LifeSpan Biosciences
catalog: LS-C245952

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (N-Terminus, Biotin) LS-C245953
LifeSpan Biosciences
catalog: LS-C245953

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (N-Terminus, FITC) LS-C245954
LifeSpan Biosciences
catalog: LS-C245954

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (N-Terminus, PE) LS-C245955
LifeSpan Biosciences
catalog: LS-C245955

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (aa590-620, AP) LS-C245956
LifeSpan Biosciences
catalog: LS-C245956

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (aa590-620, FITC) LS-C245959
LifeSpan Biosciences
catalog: LS-C245959

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (aa590-620, PE) LS-C245964
LifeSpan Biosciences
catalog: LS-C245964

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (N-Terminus, Azide-free, HRP) LS-C247887
LifeSpan Biosciences
catalog: LS-C247887

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (aa590-620, Azide-free, HRP) LS-C247888
LifeSpan Biosciences
catalog: LS-C247888

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (aa590-620) LS-C328732
LifeSpan Biosciences
catalog: LS-C328732

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (N-Terminus) LS-C330672
LifeSpan Biosciences
catalog: LS-C330672

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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SPHK2 Antibody (N-Terminus) LS-C345740
LifeSpan Biosciences
catalog: LS-C345740

domestic rabbit polyclonal
reactivity: human
application: western blot

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SPHK2 Antibody (aa52-84) LS-C356640
LifeSpan Biosciences
catalog: LS-C356640

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-SPHK2 Antibody at 1:1000 dilution + human pancreas lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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SPHK2 Antibody (C-Terminus) LS-C368517
LifeSpan Biosciences
catalog: LS-C368517

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of SPHK2 (pT614) expression in HEK293T (A); HepG2 (B) whole cell lysates.

Immunohistochemical analysis of SPHK2 (pT614) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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SPHK2 Antibody (C-Terminus) LS-C368753
LifeSpan Biosciences
catalog: LS-C368753

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of SPHK2 expression in HepG2 (A); HEK293T (B) whole cell lysates.

Immunohistochemical analysis of SPHK2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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SPHK2 Antibody (550-630 aa) LS-C381693
LifeSpan Biosciences
catalog: LS-C381693

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry

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SPHK2 Antibody (550-630 aa) LS-C386155
LifeSpan Biosciences
catalog: LS-C386155

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of SphK2 antibody
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