IHC-plus™ EIF2AK2 / PKR Antibody LS-B563
LifeSpan Biosciences
catalog: LS-B563

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-EIF2AK2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of PKR in Rat Lung cells with PKR antibody at 20 ug/ml.

Western blot of PKR in A431 whole cell lysate with PKR antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
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IHC-plus™ EIF2AK2 / PKR Antibody LS-B1352
LifeSpan Biosciences
catalog: LS-B1352

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-EIF2AK2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot of PKR in Caco-2 whole cell lysate with PKR antibody at (A) 1 and (B) 2 ug/ml.
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EIF2AK2 / PKR Antibody (aa500-550) LS-C729
LifeSpan Biosciences
catalog: LS-C729

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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EIF2AK2 / PKR Antibody (phospho-Thr446) LS-C17480
LifeSpan Biosciences
catalog: LS-C17480

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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EIF2AK2 / PKR Antibody (C-Terminus) LS-C29859
LifeSpan Biosciences
catalog: LS-C29859

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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EIF2AK2 / PKR Antibody (phospho-Thr451) LS-C17477
LifeSpan Biosciences
catalog: LS-C17477

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunocytochemistry, immunoprecipitation

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EIF2AK2 / PKR Antibody (phospho-Thr451) LS-C75195
LifeSpan Biosciences
catalog: LS-C75195

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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EIF2AK2 / PKR Antibody (phospho-Thr451) LS-C17287
LifeSpan Biosciences
catalog: LS-C17287

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry

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EIF2AK2 / PKR Antibody (aa11-42) LS-C101295
LifeSpan Biosciences
catalog: LS-C101295

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of EIF2AK2 (arrow) using PRKR Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF2AK2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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EIF2AK2 / PKR Antibody (aa519-550) LS-C101296
LifeSpan Biosciences
catalog: LS-C101296

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of EIF2AK2 (arrow) using PRKR Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF2AK2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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EIF2AK2 / PKR Antibody (aa280-329) LS-C117004
LifeSpan Biosciences
catalog: LS-C117004

domestic rabbit polyclonal
reactivity: human
application: western blot

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EIF2AK2 / PKR Antibody (phospho-Thr446) LS-C117460
LifeSpan Biosciences
catalog: LS-C117460

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from NIH/3T3 cells treated with IFN 2500U/ml 30', using PKR (Phospho-Thr446) Antibody. The lane on the right is blocked with the phospho peptide.
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EIF2AK2 / PKR Antibody (aa413-462) LS-C118226
LifeSpan Biosciences
catalog: LS-C118226

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PKR Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa, using PKR Antibody. The lane on the right is blocked with the synthesized peptide.
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EIF2AK2 / PKR Antibody (aa226-275) LS-C118322
LifeSpan Biosciences
catalog: LS-C118322

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from 293 cells, using PKR Antibody. The lane on the right is blocked with the synthesized peptide.
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EIF2AK2 / PKR Antibody (clone 1B9) LS-C133408
LifeSpan Biosciences
catalog: LS-C133408

mouse monoclonal (1B9)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M01), clone 1B9.

Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.1 ng/ml as a capture antibody.
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EIF2AK2 / PKR Antibody (clone 1D11) LS-C133409
LifeSpan Biosciences
catalog: LS-C133409

mouse monoclonal (1D11)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M02), clone 1D11.

Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 30 ug/ml]

Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.03 ng/ml as a capture antibody.
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IHC-plus™ EIF2AK2 / PKR Antibody (aa418-467) LS-B7578
LifeSpan Biosciences
catalog: LS-B7578

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-EIF2AK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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EIF2AK2 / PKR Antibody (Arg445) LS-C177695
LifeSpan Biosciences
catalog: LS-C177695

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of PKR (R445) pAb in paraffin-embedded human colon carcinoma tissue.
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EIF2AK2 / PKR Antibody (Lys440) LS-C177696
LifeSpan Biosciences
catalog: LS-C177696

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of PKR (K440) pAb in extracts from K562 cells.

IHC of PKR (K440) pAb in paraffin-embedded human breast carcinoma tissue.
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EIF2AK2 / PKR Antibody (phospho-Thr258) LS-C199468
LifeSpan Biosciences
catalog: LS-C199468

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKR (Phospho-Thr258) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with starved 24h, using PKR (Phospho-Thr258) Antibody. The lane on the right is blocked with the phospho peptide.
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EIF2AK2 / PKR Antibody (aa203-252) LS-C200494
LifeSpan Biosciences
catalog: LS-C200494

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from COLO205 cells using PKR antibody.
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EIF2AK2 / PKR Antibody (aa279-506) LS-C296128
LifeSpan Biosciences
catalog: LS-C296128

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western Blot; Sample: Human Hela cell lysate; Primary Ab: 2µg/ml Rabbit Anti-Rat PKR Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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EIF2AK2 / PKR Antibody (aa2-18) LS-C313154
LifeSpan Biosciences
catalog: LS-C313154

domestic rabbit polyclonal
reactivity: human
application: western blot

WB of EIF2AK2 / PKR antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant Human EIF2AK2, 35.0KD. (162aa tag+ M1-K154). Lane 1: Recombinant Human EIF2AK2 Protein 10ng. Lane 2: Recombinant Human EIF2AK2 Protein 5ng. Lane 3: Recombinant Human EIF2AK2 Protein 2.5ng. Lane 4: Recombinant Human EIF2AK2 Protein 1.25ng.
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EIF2AK2 / PKR Antibody (aa205-495) LS-C314531
LifeSpan Biosciences
catalog: LS-C314531

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant EIF2AK2 / PKR.

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EIF2AK2 / PKR Antibody LS-C333014
LifeSpan Biosciences
catalog: LS-C333014

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of MCF-7 cells.
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EIF2AK2 / PKR Antibody LS-C335592
LifeSpan Biosciences
catalog: LS-C335592

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extract of various cells.

Immunohistochemistry of paraffin-embedded rat brain tissue.
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EIF2AK2 / PKR Antibody (phospho-Thr451) LS-C335981
LifeSpan Biosciences
catalog: LS-C335981

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue.
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EIF2AK2 / PKR Antibody (C-Terminus) LS-C352730
LifeSpan Biosciences
catalog: LS-C352730

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of PKR expression in HeLa (A); MCF7 (B); Caco2 (C) whole cell lysates.

Immunohistochemical analysis of PKR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PKR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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EIF2AK2 / PKR Antibody (phospho-Thr451) LS-C354070
LifeSpan Biosciences
catalog: LS-C354070

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of PKR (pT451) expression in HeLa PMA-treated (A); Raw264.7 H2O2-treated (B); NIH3T3 LPS-treated (C) whole cell lysates.

Immunohistochemical analysis of PKR (pT451) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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EIF2AK2 / PKR Antibody (clone 1441CT628.33.40) LS-C356262
LifeSpan Biosciences
catalog: LS-C356262

mouse monoclonal (1441CT628.33.40)
reactivity: human
application: western blot

Western blot of lysates from A431, HepG2, SH-SY5Y cell line (from left to right), using EIF2AK2 antibody diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
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EIF2AK2 / PKR Antibody (C-Terminus) LS-C358476
LifeSpan Biosciences
catalog: LS-C358476

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of PKR expression in THP1 (A); NS-1 (B); PC12 (C) whole cell lysates.

Immunohistochemical analysis of PKR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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EIF2AK2 / PKR Antibody (aa224-502) LS-C372538
LifeSpan Biosciences
catalog: LS-C372538

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of EIF2AK2 / PKR antibody.

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EIF2AK2 / PKR Antibody (380-460 aa) LS-C380824
LifeSpan Biosciences
catalog: LS-C380824

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

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EIF2AK2 / PKR Antibody (390-470 aa) LS-C380828
LifeSpan Biosciences
catalog: LS-C380828

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry

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EIF2AK2 / PKR Antibody (200-280 aa) LS-C381391
LifeSpan Biosciences
catalog: LS-C381391

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

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EIF2AK2 / PKR Antibody (390-470 aa) LS-C385560
LifeSpan Biosciences
catalog: LS-C385560

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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EIF2AK2 / PKR Antibody (380-460 aa) LS-C385562
LifeSpan Biosciences
catalog: LS-C385562

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of PKR antibody
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EIF2AK2 / PKR Antibody (200-280 aa) LS-C385563
LifeSpan Biosciences
catalog: LS-C385563

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of PKR antibody
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