IHC-plus™ MAP2K2 / MKK2 / MEK2 Antibody (N-Terminus) LS-B4671
LifeSpan Biosciences
catalog: LS-B4671
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-MAP2K2 / MEK2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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MAP2K2 / MKK2 / MEK2 Antibody LS-C704
LifeSpan Biosciences
catalog: LS-C704
domestic rabbit polyclonal
reactivity: human
application: western blot
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MAP2K2 / MKK2 / MEK2 Antibody LS-C16485
LifeSpan Biosciences
catalog: LS-C16485
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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MAP2K2 / MKK2 / MEK2 Antibody (N-Terminus) LS-C16488
LifeSpan Biosciences
catalog: LS-C16488
mouse monoclonal
reactivity: human
application: western blot, ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (C-Terminus) LS-C81329
LifeSpan Biosciences
catalog: LS-C81329
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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MAP2K2 / MKK2 / MEK2 Antibody (Ser218) LS-C99762
LifeSpan Biosciences
catalog: LS-C99762
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of MAP2K1 (arrow) using rabbit polyclonal MAP2K1 Antibody (S217). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K1 gene (Lane 2) (Origene Technologies).
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MAP2K2 / MKK2 / MEK2 Antibody (aa262-292) LS-C99768
LifeSpan Biosciences
catalog: LS-C99768
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Fluorescent image of U251 cell stained with MEK2 (MAP2K2) Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). MEK2 immunoreactivity is localized to Cytoplasm significantly.

The anti-MAP2K2 antibody is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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MEK1 + MEK2 Antibody (phospho-Ser217/Ser221) LS-C72358
LifeSpan Biosciences
catalog: LS-C72358
domestic rabbit monoclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (clone 7F5) LS-C108836
LifeSpan Biosciences
catalog: LS-C108836
mouse monoclonal (7F5)
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry
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MEK1 + MEK2 Antibody (phospho-Ser217) LS-C117411
LifeSpan Biosciences
catalog: LS-C117411
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1/2 (Phospho-Ser217) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using MEK1/2 (Phospho-Ser217) Antibody. The lane on the right is blocked with the phospho peptide.
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MEK1 + MEK2 Antibody (phospho-Ser221) LS-C117412
LifeSpan Biosciences
catalog: LS-C117412
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1/2 (Phospho-Ser221) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from K562 cells treated with serum 20% 15' and Jurkat cells treated with EGF, using MEK1/2 (Phospho-Ser221) Antibody. The lane on the right is blocked with the phospho peptide.
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MEK1 + MEK2 Antibody (aa193-242) LS-C118178
LifeSpan Biosciences
catalog: LS-C118178
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MEK1/2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, treated with UV, using MEK1/2 Antibody. The lane on the right is blocked with the synthesized peptide.
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MAP2K2 / MKK2 / MEK2 Antibody LS-C124083
LifeSpan Biosciences
catalog: LS-C124083
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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MAP2K2 / MKK2 / MEK2 Antibody LS-C137466
LifeSpan Biosciences
catalog: LS-C137466
domestic rabbit polyclonal
reactivity: human
application: western blot
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MAP2K2 / MKK2 / MEK2 Antibody (aa36-63) LS-C164443
LifeSpan Biosciences
catalog: LS-C164443
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Mouse Map2k2 Antibody western blot of mouse liver tissue lysates (35 ug/lane). The Map2k2 antibody detected the Map2k2 protein (arrow).
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MAP2K2 / MKK2 / MEK2 Antibody (clone OTI5F6) LS-C114565
LifeSpan Biosciences
catalog: LS-C114565
mouse monoclonal (OTI5F6)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K2 antibody, and then analyzed by flow cytometry.
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MAP2K2 / MKK2 / MEK2 Antibody (clone OTI8G6) LS-C114570
LifeSpan Biosciences
catalog: LS-C114570
mouse monoclonal (OTI8G6)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K2 antibody, and then analyzed by flow cytometry.
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MAP2K2 / MKK2 / MEK2 Antibody (clone OTI5B4) LS-C174504
LifeSpan Biosciences
catalog: LS-C174504
mouse monoclonal (OTI5B4)
reactivity: human
application: western blot, immunocytochemistry

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MAP2K2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Anti-MAP2K2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K2.
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MAP2K2 / MKK2 / MEK2 Antibody (Arg388) LS-C176117
LifeSpan Biosciences
catalog: LS-C176117
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot of MEK2 (R388) pAb in extracts from HeLa cells.

IHC of MEK2 (R388) pAb in paraffin-embedded human breast carcinoma tissue.
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MAP2K2 / MKK2 / MEK2 Antibody (clone 8D10) LS-C197998
LifeSpan Biosciences
catalog: LS-C197998
mouse monoclonal (8D10)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western Blot analysis of MAP2K2 expression in transfected 293T cell line by MAP2K2 monoclonal antibody (M11), clone 8D10.Lane 1: MAP2K2 transfected lysate (Predicted MW: 44.4 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to MAP2K2 on HeLa cell . [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged MAP2K2 is 0.3 ng/ml as a capture antibody.
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MAP2K2 / MKK2 / MEK2 Antibody (aa261-310) LS-C199252
LifeSpan Biosciences
catalog: LS-C199252
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from ovary cancer cells, using MEK2 Antibody. The lane on the right is blocked with the synthesized peptide.
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MAP2K2 / MKK2 / MEK2 Antibody (aa359-372) LS-C205037
LifeSpan Biosciences
catalog: LS-C205037
domestic goat polyclonal
reactivity: human
application: western blot

MAP2K2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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MAP2K2 / MKK2 / MEK2 Antibody (aa36-63, AP) LS-C223164
LifeSpan Biosciences
catalog: LS-C223164
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (aa36-63, APC) LS-C223165
LifeSpan Biosciences
catalog: LS-C223165
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (aa36-63, FITC) LS-C223166
LifeSpan Biosciences
catalog: LS-C223166
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (aa36-63, Biotin) LS-C223167
LifeSpan Biosciences
catalog: LS-C223167
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (aa36-63, PE) LS-C223169
LifeSpan Biosciences
catalog: LS-C223169
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (aa36-63, Azide-free, HRP) LS-C223170
LifeSpan Biosciences
catalog: LS-C223170
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (phospho-Ser222, AP) LS-C223171
LifeSpan Biosciences
catalog: LS-C223171
domestic rabbit polyclonal
reactivity: human
conjugate: AP
modification: phosphorylated
application: ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (phospho-Ser222, APC) LS-C223172
LifeSpan Biosciences
catalog: LS-C223172
domestic rabbit polyclonal
reactivity: human
conjugate: APC
modification: phosphorylated
application: ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (phospho-Ser222, Biotin) LS-C223173
LifeSpan Biosciences
catalog: LS-C223173
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
modification: phosphorylated
application: ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (phospho-Ser222, Azide-free, HRP) LS-C223174
LifeSpan Biosciences
catalog: LS-C223174
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
modification: phosphorylated
application: ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (phospho-Ser222, FITC) LS-C223175
LifeSpan Biosciences
catalog: LS-C223175
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
modification: phosphorylated
application: ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (phospho-Ser222, PE) LS-C223176
LifeSpan Biosciences
catalog: LS-C223176
domestic rabbit polyclonal
reactivity: human
conjugate: PE
modification: phosphorylated
application: ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (aa262-292, AP) LS-C225879
LifeSpan Biosciences
catalog: LS-C225879
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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MAP2K2 / MKK2 / MEK2 Antibody (aa262-292, APC) LS-C225880
LifeSpan Biosciences
catalog: LS-C225880
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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MAP2K2 / MKK2 / MEK2 Antibody (aa262-292, Biotin) LS-C225883
LifeSpan Biosciences
catalog: LS-C225883
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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MAP2K2 / MKK2 / MEK2 Antibody (aa262-292, FITC) LS-C225884
LifeSpan Biosciences
catalog: LS-C225884
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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MAP2K2 / MKK2 / MEK2 Antibody (aa262-292, Azide-free, HRP) LS-C225885
LifeSpan Biosciences
catalog: LS-C225885
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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MAP2K2 / MKK2 / MEK2 Antibody (aa262-292, PE) LS-C225886
LifeSpan Biosciences
catalog: LS-C225886
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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MAP2K2 / MKK2 / MEK2 Antibody (aa1-50) LS-C286104
LifeSpan Biosciences
catalog: LS-C286104
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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MAP2K2 / MKK2 / MEK2 Antibody (aa1-50) LS-C286830
LifeSpan Biosciences
catalog: LS-C286830
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
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MAP2K2 / MKK2 / MEK2 Antibody (aa350-400) LS-C288899
LifeSpan Biosciences
catalog: LS-C288899
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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MAP2K2 / MKK2 / MEK2 Antibody (aa44-313) LS-C295389
LifeSpan Biosciences
catalog: LS-C295389
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western Blot; Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Kidney Tissue; Lane3: Rat Brain Tissue.

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MAP2K2 / MKK2 / MEK2 Antibody LS-C308877
LifeSpan Biosciences
catalog: LS-C308877
mouse polyclonal
reactivity: human
application: western blot
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MAP2K2 / MKK2 / MEK2 Antibody LS-C310920
LifeSpan Biosciences
catalog: LS-C310920
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
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MAP2K2 / MKK2 / MEK2 Antibody LS-C310921
LifeSpan Biosciences
catalog: LS-C310921
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
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MAP2K2 / MKK2 / MEK2 Antibody (aa2-20) LS-C313370
LifeSpan Biosciences
catalog: LS-C313370
domestic rabbit polyclonal
reactivity: human
application: western blot

WB of MAP2K2 / MKK2 / MEK2 antibody. All lanes: MAP2K2 at 0.5ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug. Lane 2: Rate Kidney Tissue Lysate at 40ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: COLO320 Whole Cell Lysate at 40ug. Lane 5: PC12 Whole Cell Lysate at 40ug. Lane 6: JURKAT Whole Cell Lysate at 40ug. Lane 7: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 50KD. Observed bind size: 50KD.
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MAP2K2 / MKK2 / MEK2 Antibody LS-C192656
LifeSpan Biosciences
catalog: LS-C192656
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of MEK2 pAb in extracts from mouse brain tissue.
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MAP2K2 / MKK2 / MEK2 Antibody (aa262-292) LS-C327876
LifeSpan Biosciences
catalog: LS-C327876
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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MAP2K2 / MKK2 / MEK2 Antibody (phospho-Ser222) LS-C329797
LifeSpan Biosciences
catalog: LS-C329797
domestic rabbit polyclonal
reactivity: human
application: ELISA
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MAP2K2 / MKK2 / MEK2 Antibody (aa36-63) LS-C329799
LifeSpan Biosciences
catalog: LS-C329799
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
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MAP2K2 / MKK2 / MEK2 Antibody LS-C330876
LifeSpan Biosciences
catalog: LS-C330876
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of mouse brain, using MAP2K2 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunohistochemistry of paraffin-embedded human kidney using MAP2K2 antibodyat dilution of 1:100 (40x lens).
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MAP2K2 / MKK2 / MEK2 Antibody (clone OTI1A2) LS-C337945
LifeSpan Biosciences
catalog: LS-C337945
mouse monoclonal (OTI1A2)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of extracts (35ug) from 9 different cell lines by using anti-MAP2K2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC of paraffin-embedded Human Kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-MAP2K2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K2.
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MAP2K2 / MKK2 / MEK2 Antibody (clone OTI5G8) LS-C337955
LifeSpan Biosciences
catalog: LS-C337955
mouse monoclonal (OTI5G8)
reactivity: human
application: western blot, immunocytochemistry

Western blot of extracts (35ug) from 9 different cell lines by using anti-MAP2K2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Anti-MAP2K2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K2.
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MAP2K2 / MKK2 / MEK2 Antibody (phospho-Thr394) LS-C354067
LifeSpan Biosciences
catalog: LS-C354067
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of MKK2 (pT394) expression in HeLa UV-treated (A); HepG2 UV-treated (B) whole cell lysates.

Immunohistochemical analysis of MKK2 (pT394) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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MAP2K2 / MKK2 / MEK2 Antibody (aa86-331) LS-C374779
LifeSpan Biosciences
catalog: LS-C374779
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Western Blot; Sample: Lane1: Human Jurkat cells; Lane2: Human Hela cels.

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MAP2K2 / MKK2 / MEK2 Antibody (340-420 aa) LS-C384496
LifeSpan Biosciences
catalog: LS-C384496
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation

Western blot of MEK-2 antibody
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MAP2K2 / MKK2 / MEK2 Antibody LS-C387834
LifeSpan Biosciences
catalog: LS-C387834
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Immunohistochemistry of MEK-2 antibody

Western blot of MEK-2 antibody
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