MAPK6 / ERK3 Antibody LS-C96010
LifeSpan Biosciences
catalog: LS-C96010
chicken polyclonal
reactivity: human
application: ELISA
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MAPK6 / ERK3 Antibody (aa155-204) LS-C117892
LifeSpan Biosciences
catalog: LS-C117892
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HeLa cells, using p97 MAPK Antibody. The lane on the right is blocked with the synthesized peptide.
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MAPK6 / ERK3 Antibody (aa395-444) LS-C135383
LifeSpan Biosciences
catalog: LS-C135383
domestic rabbit polyclonal
reactivity: human
application: western blot
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MAPK6 / ERK3 Antibody (clone 4E8) LS-C169344
LifeSpan Biosciences
catalog: LS-C169344
mouse monoclonal (4E8)
reactivity: human
application: western blot, ELISA, flow cytometry
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MAPK6 / ERK3 Antibody (clone OTI5E1) LS-C114368
LifeSpan Biosciences
catalog: LS-C114368
mouse monoclonal (OTI5E1)
reactivity: human
application: western blot, immunocytochemistry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.

Anti-MAPK6 mouse monoclonal antibody immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6.
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MAPK6 / ERK3 Antibody (clone 1G6) LS-C197995
LifeSpan Biosciences
catalog: LS-C197995
mouse monoclonal (1G6)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western Blot analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.Lane 1: MAPK6 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to MAPK6 on HeLa cell . [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged MAPK6 is approximately 0.03 ng/ml as a capture antibody.
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MAPK6 / ERK3 Antibody (phospho-Ser189) LS-C198929
LifeSpan Biosciences
catalog: LS-C198929
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ERK3 (Phospho-Ser189) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from mouse brain, using ERK3 (Phospho-Ser189) Antibody. The lane on the right is blocked with the phospho peptide.
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MAPK6 / ERK3 Antibody (aa136-185) LS-C200455
LifeSpan Biosciences
catalog: LS-C200455
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from Jurkat cells using p97 MAPK antibody.
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MAPK6 / ERK3 Antibody (aa426-475) LS-C200456
LifeSpan Biosciences
catalog: LS-C200456
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HeLa cells using p97 MAPK antibody.
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MAPK6 / ERK3 Antibody (phospho-Ser189, AP) LS-C213956
LifeSpan Biosciences
catalog: LS-C213956
domestic rabbit polyclonal
reactivity: human
conjugate: AP
modification: phosphorylated
application: ELISA, immunohistochemistry
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MAPK6 / ERK3 Antibody (phospho-Ser189, APC) LS-C213957
LifeSpan Biosciences
catalog: LS-C213957
domestic rabbit polyclonal
reactivity: human
conjugate: APC
modification: phosphorylated
application: ELISA, immunohistochemistry
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MAPK6 / ERK3 Antibody (phospho-Ser189, Biotin) LS-C213958
LifeSpan Biosciences
catalog: LS-C213958
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
modification: phosphorylated
application: ELISA, immunohistochemistry
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MAPK6 / ERK3 Antibody (phospho-Ser189, FITC) LS-C213959
LifeSpan Biosciences
catalog: LS-C213959
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
modification: phosphorylated
application: ELISA, immunohistochemistry
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MAPK6 / ERK3 Antibody (phospho-Ser189, Azide-free, HRP) LS-C213961
LifeSpan Biosciences
catalog: LS-C213961
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
modification: phosphorylated
application: ELISA, immunohistochemistry
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MAPK6 / ERK3 Antibody (phospho-Ser189, PE) LS-C213962
LifeSpan Biosciences
catalog: LS-C213962
domestic rabbit polyclonal
reactivity: human
conjugate: PE
modification: phosphorylated
application: ELISA, immunohistochemistry
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IHC-plus™ MAPK6 / ERK3 Antibody (aa461-510) LS-B9961
LifeSpan Biosciences
catalog: LS-B9961
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-MAPK6 / ERK3 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of MCF7 cells, using p97 MAPK Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from K562 cells, using p97 MAPK Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ MAPK6 / ERK3 Antibody (aa683-694) LS-B10395
LifeSpan Biosciences
catalog: LS-B10395
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

MAPK6 antibody (1.5 ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).

Anti-MAPK6 / ERK3 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

MAPK6 / ERK3 antibody (1.5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).
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MAPK6 / ERK3 Antibody (aa671-721) LS-C289376
LifeSpan Biosciences
catalog: LS-C289376
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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MAPK6 / ERK3 Antibody (phospho-Ser189) LS-C192566
LifeSpan Biosciences
catalog: LS-C192566
domestic rabbit polyclonal
reactivity: human
application: western blot
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MAPK6 / ERK3 Antibody (phospho-Ser189) LS-C321177
LifeSpan Biosciences
catalog: LS-C321177
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
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MAPK6 / ERK3 Antibody (N-Terminus) LS-C345532
LifeSpan Biosciences
catalog: LS-C345532
domestic rabbit polyclonal
reactivity: human
application: western blot
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MAPK6 / ERK3 Antibody (Internal) LS-C354065
LifeSpan Biosciences
catalog: LS-C354065
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of ERK3 expression in HeLa (A); NIH3T3 (B) whole cell lysates.

Immunohistochemical analysis of ERK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of ERK3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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MAPK6 / ERK3 Antibody (130-210 aa) LS-C381331
LifeSpan Biosciences
catalog: LS-C381331
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
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MAPK6 / ERK3 Antibody (430-510 aa, Internal) LS-C383407
LifeSpan Biosciences
catalog: LS-C383407
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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MAPK6 / ERK3 Antibody (130-210 aa) LS-C383409
LifeSpan Biosciences
catalog: LS-C383409
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of ERK 3 antibody
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MAPK6 / ERK3 Antibody LS-C387766
LifeSpan Biosciences
catalog: LS-C387766
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Immunohistochemistry of ERK 3 antibody

Western blot of ERK 3 antibody
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MAPK6 / ERK3 Antibody (aa520-721) LS-C407877
LifeSpan Biosciences
catalog: LS-C407877
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

MAPK6 antibody Western blot. All lanes: Anti MAPK6 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Lane 4: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 68 kD. Observed band size: 100 kD.

MAPK6 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
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