PRKCZ / PKC-Zeta Antibody (aa579-592) LS-C26209
LifeSpan Biosciences
catalog: LS-C26209

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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PRKCZ / PKC-Zeta Antibody (aa577-592) LS-C26196
LifeSpan Biosciences
catalog: LS-C26196

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

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PRKCZ / PKC-Zeta Antibody (Internal) LS-C26198
LifeSpan Biosciences
catalog: LS-C26198

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunoprecipitation

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PRKCZ / PKC-Zeta Antibody (C-Terminus) LS-C26208
LifeSpan Biosciences
catalog: LS-C26208

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

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PRKCZ / PKC-Zeta Antibody (phospho-Thr410/Thr403) LS-C26210
LifeSpan Biosciences
catalog: LS-C26210

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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PRKCZ / PKC-Zeta Antibody LS-C26200
LifeSpan Biosciences
catalog: LS-C26200

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

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PRKCZ / PKC-Zeta Antibody (C-Terminus) LS-C38547
LifeSpan Biosciences
catalog: LS-C38547

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, ELISA, immunoprecipitation, radioimmunoassay

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PRKCZ / PKC-Zeta Antibody LS-C38548
LifeSpan Biosciences
catalog: LS-C38548

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, ELISA, immunocytochemistry

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PRKCZ / PKC-Zeta Antibody (N-Terminus) LS-C40401
LifeSpan Biosciences
catalog: LS-C40401

domestic rabbit polyclonal
reactivity: human
application: western blot

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PRKCZ / PKC-Zeta Antibody LS-C83670
LifeSpan Biosciences
catalog: LS-C83670

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

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PRKCZ / PKC-Zeta Antibody LS-C91502
LifeSpan Biosciences
catalog: LS-C91502

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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PRKCZ / PKC-Zeta Antibody (aa166-197) LS-C98935
LifeSpan Biosciences
catalog: LS-C98935

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-PKCzeta antibody in placenta lysate. PKCzeta (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PKC zeta antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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PRKCZ / PKC-Zeta Antibody (phospho-Thr410) LS-C124446
LifeSpan Biosciences
catalog: LS-C124446

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry

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PRKCZ / PKC-Zeta Antibody LS-C124447
LifeSpan Biosciences
catalog: LS-C124447

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

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PRKCZ / PKC-Zeta Antibody (aa1-30) LS-C164554
LifeSpan Biosciences
catalog: LS-C164554

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Mouse Prkcz Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The Prkcz antibody detected the Prkcz protein (arrow).
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PRKCZ / PKC-Zeta Antibody (clone S19-V, FITC) LS-C170833
LifeSpan Biosciences
catalog: LS-C170833

domestic rabbit polyclonal (S19-V)
reactivity: human
conjugate: FITC
application: flow cytometry

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PRKCZ / PKC-Zeta Antibody (Pro556) LS-C176454
LifeSpan Biosciences
catalog: LS-C176454

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of PKC (P556) pAb in extracts from COS7 cells treated with PMA 125ng/ml 30'.

IHC of PKC (P556) pAb in paraffin-embedded human brain tissue.
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PRKCZ / PKC-Zeta Antibody (phospho-Thr410) LS-C177941
LifeSpan Biosciences
catalog: LS-C177941

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of p-PKC (T410) pAb in extracts from NIH/3T3 cells.

IHC of p-PKC (T410) pAb in paraffin-embedded human lung carcinoma tissue.
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PRKCZ / PKC-Zeta Antibody (clone 2D1) LS-C197992
LifeSpan Biosciences
catalog: LS-C197992

mouse monoclonal (2D1)
reactivity: human
application: western blot, ELISA

Western Blot analysis of PRKCZ expression in transfected 293T cell line by PRKCZ monoclonal antibody (M01), clone 2D1.Lane 1: PRKCZ transfected lysate(67.7 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged PRKCZ is approximately 0.1 ng/ml as a capture antibody.

Western blot analysis of PRKCZ over-expressed 293 cell line, cotransfected with PRKCZ Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCZ monoclonal antibody (M01) clone 2D1 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
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PRKCZ / PKC-Zeta Antibody (phospho-Thr410) LS-C199456
LifeSpan Biosciences
catalog: LS-C199456

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HeLa cells, using PKC zeta (Phospho-Thr410) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PKC zeta (Phospho-Thr410) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using PKC zeta (Phospho-Thr410) Antibody. The lane on the right is blocked with the phospho peptide.
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PRKCZ / PKC-Zeta Antibody (phospho-Thr560) LS-C199457
LifeSpan Biosciences
catalog: LS-C199457

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain, using PKC zeta (Phospho-Thr560) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using PKC zeta (Phospho-Thr560) Antibody. The lane on the right is blocked with the phospho peptide.
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PRKCZ / PKC-Zeta Antibody (aa166-197, AP) LS-C266419
LifeSpan Biosciences
catalog: LS-C266419

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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PRKCZ / PKC-Zeta Antibody (aa166-197, APC) LS-C266420
LifeSpan Biosciences
catalog: LS-C266420

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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PRKCZ / PKC-Zeta Antibody (aa166-197, Biotin) LS-C266421
LifeSpan Biosciences
catalog: LS-C266421

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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PRKCZ / PKC-Zeta Antibody (aa166-197, FITC) LS-C266422
LifeSpan Biosciences
catalog: LS-C266422

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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PRKCZ / PKC-Zeta Antibody (aa166-197, Azide-free, HRP) LS-C266423
LifeSpan Biosciences
catalog: LS-C266423

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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PRKCZ / PKC-Zeta Antibody (aa166-197, PE) LS-C266424
LifeSpan Biosciences
catalog: LS-C266424

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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PRKCZ / PKC-Zeta Antibody (aa1-30, AP) LS-C284769
LifeSpan Biosciences
catalog: LS-C284769

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: AP
application: western blot, ELISA

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PRKCZ / PKC-Zeta Antibody (aa1-30, APC) LS-C284770
LifeSpan Biosciences
catalog: LS-C284770

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: APC
application: western blot, ELISA

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PRKCZ / PKC-Zeta Antibody (aa1-30, FITC) LS-C284771
LifeSpan Biosciences
catalog: LS-C284771

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA

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PRKCZ / PKC-Zeta Antibody (aa1-30, Biotin) LS-C284772
LifeSpan Biosciences
catalog: LS-C284772

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA

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PRKCZ / PKC-Zeta Antibody (aa1-30, PE) LS-C284775
LifeSpan Biosciences
catalog: LS-C284775

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: PE
application: western blot, ELISA

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PRKCZ / PKC-Zeta Antibody (aa1-30, Azide-free, HRP) LS-C284776
LifeSpan Biosciences
catalog: LS-C284776

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA

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PRKCZ / PKC-Zeta Antibody (aa404-591) LS-C296114
LifeSpan Biosciences
catalog: LS-C296114

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Human 293T cell lysate; Primary Ab: 3µg/ml Rabbit Anti-Human PKCz Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PRKCZ / PKC-Zeta Antibody (aa254-484) LS-C296117
LifeSpan Biosciences
catalog: LS-C296117

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant PRKCZ / PKC-Zeta. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PRKCZ / PKC-Zeta Antibody (aa166-197) LS-C324070
LifeSpan Biosciences
catalog: LS-C324070

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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PRKCZ / PKC-Zeta Antibody (aa1-30) LS-C330013
LifeSpan Biosciences
catalog: LS-C330013

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

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PRKCZ / PKC-Zeta Antibody LS-C334239
LifeSpan Biosciences
catalog: LS-C334239

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of HeLa cells.
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IHC-plus™ PRKCZ / PKC-Zeta Antibody (Internal, clone EP1490(2)) LS-B6294
LifeSpan Biosciences
catalog: LS-B6294

domestic rabbit monoclonal (EP1490(2))
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PRKCZ / PKC-Zeta antibody IHC of human brain cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
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PRKCZ / PKC-Zeta Antibody (phospho-Thr560) LS-C352724
LifeSpan Biosciences
catalog: LS-C352724

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of PKC zeta (pT560) expression in HeLa UV-treated (A); A431 (B); A549 UV-treated (C); SP2/0 UV-treated (D); PC12 UV-treated (E) whole cell lysates.
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PRKCZ / PKC-Zeta Antibody (C-Terminus) LS-C354069
LifeSpan Biosciences
catalog: LS-C354069

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of PKC zeta expression in HeLa (A); NIH3T3 (B); PC12 (C); rat kidney (D); rat heart (E) whole cell lysates.

Immunohistochemical analysis of PKC zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PKC zeta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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IHC-plus™ PRKCZ / PKC-Zeta Antibody (aa376-425) LS-B12514
LifeSpan Biosciences
catalog: LS-B12514

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence analysis of HeLa cells, using PKC zeta Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, treated with PMA 125ng/ml 30', using PKC zeta Antibody. The lane on the right is blocked with the synthesized peptide.
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PRKCZ / PKC-Zeta Antibody (350-430 aa) LS-C380825
LifeSpan Biosciences
catalog: LS-C380825

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Phospho-PKC zeta (T410) antibody
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PRKCZ / PKC-Zeta Antibody (500-580 aa) LS-C380826
LifeSpan Biosciences
catalog: LS-C380826

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-PKC zeta (T560) antibody
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PRKCZ / PKC-Zeta Antibody (350-430 aa) LS-C385538
LifeSpan Biosciences
catalog: LS-C385538

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of PKC zeta antibody
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PRKCZ / PKC-Zeta Antibody (500-580 aa) LS-C385540
LifeSpan Biosciences
catalog: LS-C385540

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of PKC zeta antibody
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