mCherry Antibody LS-C204825
LifeSpan Biosciences
catalog: LS-C204825

chicken polyclonal
reactivity: rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 5

Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.

HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
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mCherry Antibody LS-C204207
LifeSpan Biosciences
catalog: LS-C204207

domestic goat polyclonal
reactivity: rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 2

Western blot. Anti-mCherry antibody at 1:1000 dilution. 293 cells transfected with myc-mCherry. Lysates at 100 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

293 HEK cells transduced with Ad mCherry Rab1a and stained with anti-mCherry
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mCherry Antibody LS-C147181
LifeSpan Biosciences
catalog: LS-C147181

domestic rabbit polyclonal
application: western blot
citations: 1

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mCherry Antibody LS-C204826
LifeSpan Biosciences
catalog: LS-C204826

domestic rabbit polyclonal
application: western blot, immunocytochemistry
citations: 1

Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, in the lane marked plus. HEK293 cells which were not transfected with this vector show no protein band in lane marked minus.

HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
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mCherry Antibody LS-C183447
LifeSpan Biosciences
catalog: LS-C183447

domestic rabbit polyclonal
application: western blot

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mCherry Antibody (clone 1C51) LS-C204827
LifeSpan Biosciences
catalog: LS-C204827

mouse monoclonal (1C51)
application: western blot, immunocytochemistry

Blot of crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector in lane labeled +. The - lane is a blot of an equal amount of protein extract from untransfected HEK293 cells. mCherry antibody binds a major band running at ~28kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected HEK293 cells.

Shows HEK293 cells transfected with mCherry and visualized in red. The cells were stained with mCherry antibody in the green channel, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and the mCherry antibody antibody. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue). Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
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mCherry Antibody (clone 1) LS-C305988
LifeSpan Biosciences
catalog: LS-C305988

mouse monoclonal (1)
application: western blot

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mCherry Tag Antibody LS-C342279
LifeSpan Biosciences
catalog: LS-C342279

mouse monoclonal
application: western blot

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mCherry Antibody (clone Mix) LS-C387413
LifeSpan Biosciences
catalog: LS-C387413

mouse monoclonal (Mix)
application: western blot

Western blot of mCherry antibody
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mCherry Tag Antibody LS-C387465
LifeSpan Biosciences
catalog: LS-C387465

domestic rabbit polyclonal
application: western blot

Western blot of mCherry-Tag antibody
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mCherry Tag Antibody LS-C387472
LifeSpan Biosciences
catalog: LS-C387472

mouse polyclonal
application: western blot

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