PRKAA2 / AMPK Alpha 2 Antibody (aa453-483) LS-C100607
LifeSpan Biosciences
catalog: LS-C100607

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of PRKAA2 (arrow) using rabbit polyclonal PRKAA2 Antibody (RB11657). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PRKAA2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (C-Terminus) LS-C122646
LifeSpan Biosciences
catalog: LS-C122646

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

PRKAA1 + PRKAA2 Antibody (aa140-189) LS-C117514
LifeSpan Biosciences
catalog: LS-C117514

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AMPK alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COS7 cells, treated with Adriamycin 0.5ng/ml 24h, using AMPK alpha Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa353-367) LS-C20490
LifeSpan Biosciences
catalog: LS-C20490

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (C-Terminus) LS-C20492
LifeSpan Biosciences
catalog: LS-C20492

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (C-Terminus) LS-C20493
LifeSpan Biosciences
catalog: LS-C20493

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (phospho-Ser175) LS-C46806
LifeSpan Biosciences
catalog: LS-C46806

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody LS-C83009
LifeSpan Biosciences
catalog: LS-C83009

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

PRKAA1 + PRKAA2 Antibody (phospho-Ser485) LS-C11847
LifeSpan Biosciences
catalog: LS-C11847

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa476-507) LS-C100728
LifeSpan Biosciences
catalog: LS-C100728

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of anti-AMPK alpha2 C-term antibody in mouse heart tissue lysate. AMPK alpha2 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa323-372) LS-C107761
LifeSpan Biosciences
catalog: LS-C107761

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody LS-C122651
LifeSpan Biosciences
catalog: LS-C122651

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (C-Terminus) LS-C126725
LifeSpan Biosciences
catalog: LS-C126725

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody LS-C126726
LifeSpan Biosciences
catalog: LS-C126726

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa382-392) LS-C139628
LifeSpan Biosciences
catalog: LS-C139628

domestic goat polyclonal
reactivity: human
application: western blot, ELISA

PRKAA2 antibody (1 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody LS-C146865
LifeSpan Biosciences
catalog: LS-C146865

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody LS-C146866
LifeSpan Biosciences
catalog: LS-C146866

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody LS-C146888
LifeSpan Biosciences
catalog: LS-C146888

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (Thr172) LS-C162143
LifeSpan Biosciences
catalog: LS-C162143

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

PRKAA2 (Thr172) Antibody western blot of K562 cell line lysates (35 ug/lane). The PRKAA2 antibody detected the PRKAA2 protein (arrow).

PRKAA2 (Thr172) antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.This data demonstrates the use of the PRKAA2 (Thr172) antibody for immunohistochemistry.

PRKAA2 (Thr172) Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody LS-C162144
LifeSpan Biosciences
catalog: LS-C162144

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry

Fluorescent image of HeLa cell stained with PRKAA2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRKAA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). PRKAA2 immunoreactivity is localized to Cytoplasm significantly.

PRKAA2 antibody western blot of CEM cell line lysates (35 ug/lane). The PRKAA2 antibody detected the PRKAA2 protein (arrow).
quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa29-510) LS-C185542
LifeSpan Biosciences
catalog: LS-C185542

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Immunofluorescence of paraformaldehyde-fixed A431 using AMPK alpha 2 antibody at 1:200 dilution.

Sample (30 ug whole cell lysate). A: A431, B: MOLT4 . 7.5% SDS PAGE. PRKAA2 antibody diluted at 1:1000

IHC of paraffin-embedded A549 xenograft using AMPK alpha 2 antibody at 1:500 dilution.
quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (clone 1G8) LS-C197983
LifeSpan Biosciences
catalog: LS-C197983

mouse monoclonal (1G8)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western Blot analysis of PRKAA2 expression in transfected 293T cell line by PRKAA2 monoclonal antibody (M02), clone 1G8.Lane 1: PRKAA2 transfected lysate(62.3 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to PRKAA2 on HeLa cell . [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged PRKAA2 is approximately 0.03 ng/ml as a capture antibody.
quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (clone 206CT5.4.2, AP) LS-C212519
LifeSpan Biosciences
catalog: LS-C212519

mouse monoclonal (206CT5.4.2)
reactivity: human
conjugate: AP
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (clone 206CT5.4.2, APC) LS-C212520
LifeSpan Biosciences
catalog: LS-C212520

mouse monoclonal (206CT5.4.2)
reactivity: human
conjugate: APC
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (clone 206CT5.4.2, Biotin) LS-C212521
LifeSpan Biosciences
catalog: LS-C212521

mouse monoclonal (206CT5.4.2)
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (clone 206CT5.4.2, FITC) LS-C212522
LifeSpan Biosciences
catalog: LS-C212522

mouse monoclonal (206CT5.4.2)
reactivity: human
conjugate: FITC
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (clone 206CT5.4.2, Azide-free, HRP) LS-C212523
LifeSpan Biosciences
catalog: LS-C212523

mouse monoclonal (206CT5.4.2)
reactivity: human
conjugate: HRP
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (clone 206CT5.4.2, PE) LS-C212524
LifeSpan Biosciences
catalog: LS-C212524

mouse monoclonal (206CT5.4.2)
reactivity: human
conjugate: PE
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (Thr172, AP) LS-C216933
LifeSpan Biosciences
catalog: LS-C216933

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (Thr172, APC) LS-C216934
LifeSpan Biosciences
catalog: LS-C216934

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (Thr172, Azide-free, HRP) LS-C216937
LifeSpan Biosciences
catalog: LS-C216937

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (Thr172, PE) LS-C216940
LifeSpan Biosciences
catalog: LS-C216940

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa476-507, AP) LS-C218623
LifeSpan Biosciences
catalog: LS-C218623

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa476-507, Biotin) LS-C218624
LifeSpan Biosciences
catalog: LS-C218624

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa476-507, APC) LS-C218625
LifeSpan Biosciences
catalog: LS-C218625

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa476-507, FITC) LS-C218626
LifeSpan Biosciences
catalog: LS-C218626

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa476-507, Azide-free, HRP) LS-C218627
LifeSpan Biosciences
catalog: LS-C218627

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa476-507, PE) LS-C218628
LifeSpan Biosciences
catalog: LS-C218628

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa453-483, AP) LS-C218629
LifeSpan Biosciences
catalog: LS-C218629

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa453-483, APC) LS-C218630
LifeSpan Biosciences
catalog: LS-C218630

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa453-483, Biotin) LS-C218631
LifeSpan Biosciences
catalog: LS-C218631

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa453-483, FITC) LS-C218632
LifeSpan Biosciences
catalog: LS-C218632

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa453-483, Azide-free, HRP) LS-C218633
LifeSpan Biosciences
catalog: LS-C218633

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa453-483, PE) LS-C218634
LifeSpan Biosciences
catalog: LS-C218634

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa350-400) LS-C287395
LifeSpan Biosciences
catalog: LS-C287395

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (Thr172) LS-C324292
LifeSpan Biosciences
catalog: LS-C324292

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa476-507) LS-C326834
LifeSpan Biosciences
catalog: LS-C326834

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa453-483) LS-C326836
LifeSpan Biosciences
catalog: LS-C326836

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (clone 206CT5.4.2) LS-C329178
LifeSpan Biosciences
catalog: LS-C329178

mouse monoclonal (206CT5.4.2)
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody LS-C331110
LifeSpan Biosciences
catalog: LS-C331110

mouse polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of various cell lines, using PRKAA2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody LS-C349072
LifeSpan Biosciences
catalog: LS-C349072

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of A549 cells.
quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa252-493) LS-C374746
LifeSpan Biosciences
catalog: LS-C374746

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Lane1: Porcine Skeletal Muscle Tissue; Lane2: Human Hela Cells; Lane3: Human MCF7 Cells; Lane4: Human HepG2 Cells.

quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody (aa343-552) LS-C378047
LifeSpan Biosciences
catalog: LS-C378047

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: PRKAA2 antibody at 8 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: MCF-7 whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 62 kDa. Observed band size: 62 kDa.

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
quantity:
price:
to the supplier

PRKAA2 / AMPK Alpha 2 Antibody LS-C387763
LifeSpan Biosciences
catalog: LS-C387763

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of AMPKalpha2 antibody
quantity:
price:
to the supplier