PRKAA1 / AMPK Alpha 1 Antibody (Ser487) LS-C100814
LifeSpan Biosciences
catalog: LS-C100814
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of PRKAA1 (arrow) using rabbit polyclonal PRKAA1 Antibody (S246). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA1 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PRKAA1-pS487, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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PRKAA1 / AMPK Alpha 1 Antibody (phospho-Ser496) LS-C117289
LifeSpan Biosciences
catalog: LS-C117289
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using AMPK1 (Phospho-Ser485) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with heat shock, using AMPK1 (Phospho-Ser485) Antibody. The lane on the right is blocked with the phospho peptide.
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PRKAA1 / AMPK Alpha 1 Antibody (aa451-500) LS-C118044
LifeSpan Biosciences
catalog: LS-C118044
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AMPK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HT29 cells, using AMPK1 Antibody. The lane on the right is blocked with the synthesized peptide.
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PRKAA1 / AMPK Alpha 1 Antibody (aa376-392) LS-C11841
LifeSpan Biosciences
catalog: LS-C11841
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (C-Terminus) LS-C11842
LifeSpan Biosciences
catalog: LS-C11842
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (phospho-Ser485) LS-C34952
LifeSpan Biosciences
catalog: LS-C34952
domestic rabbit monoclonal
reactivity: human
modification: phosphorylated
application: western blot
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PRKAA1 / AMPK Alpha 1 Antibody LS-C83008
LifeSpan Biosciences
catalog: LS-C83008
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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PRKAA1 / AMPK Alpha 1 Antibody (aa479-510) LS-C100725
LifeSpan Biosciences
catalog: LS-C100725
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of anti-AMPK alpha 1 C-term antibody in mouse liver lysate. AMPK alpha 1 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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PRKAA1 / AMPK Alpha 1 Antibody (aa251-300) LS-C111536
LifeSpan Biosciences
catalog: LS-C111536
domestic rabbit polyclonal
reactivity: human
application: western blot
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PRKAA1 / AMPK Alpha 1 Antibody (N-Terminus) LS-C122645
LifeSpan Biosciences
catalog: LS-C122645
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
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PRKAA1 / AMPK Alpha 1 Antibody (phospho-Thr174) LS-C122649
LifeSpan Biosciences
catalog: LS-C122649
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry
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PRKAA1 / AMPK Alpha 1 Antibody LS-C122650
LifeSpan Biosciences
catalog: LS-C122650
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
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PRKAA1 / AMPK Alpha 1 Antibody (N-Terminus) LS-C126727
LifeSpan Biosciences
catalog: LS-C126727
domestic rabbit monoclonal
reactivity: human
application: western blot, immunoprecipitation, flow cytometry
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PRKAA1 / AMPK Alpha 1 Antibody (clone 4D9) LS-C133393
LifeSpan Biosciences
catalog: LS-C133393
mouse monoclonal (4D9)
reactivity: human
application: western blot, ELISA

PRKAA1 monoclonal antibody (M02), clone 4D9 Western blot of PRKAA1 expression in K-562.

Detection limit for recombinant GST tagged PRKAA1 is approximately 3 ng/ml as a capture antibody.
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PRKAA1 / AMPK Alpha 1 Antibody (clone 4D8) LS-C133394
LifeSpan Biosciences
catalog: LS-C133394
mouse monoclonal (4D8)
reactivity: human
application: western blot, ELISA, immunocytochemistry

PRKAA1 monoclonal antibody (M03), clone 4D8 Western blot of PRKAA1 expression in K-562.

Immunofluorescence of monoclonal antibody to PRKAA1 on HeLa cell. [antibody concentration 10 ug/ml]
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PRKAA1 / AMPK Alpha 1 Antibody (aa95-144) LS-C146275
LifeSpan Biosciences
catalog: LS-C146275
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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PRKAA1 / AMPK Alpha 1 Antibody LS-C146862
LifeSpan Biosciences
catalog: LS-C146862
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody LS-C146863
LifeSpan Biosciences
catalog: LS-C146863
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody LS-C146864
LifeSpan Biosciences
catalog: LS-C146864
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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PRKAA1 / AMPK Alpha 1 Antibody LS-C147063
LifeSpan Biosciences
catalog: LS-C147063
domestic rabbit polyclonal
reactivity: human
application: western blot
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PRKAA1 / AMPK Alpha 1 Antibody LS-C162139
LifeSpan Biosciences
catalog: LS-C162139
mouse monoclonal
reactivity: human
application: western blot

PRKAA1 Antibody western blot of K562 cell line lysates (35 ug/lane). The PRKAA1 antibody detected the PRKAA1 protein (arrow).
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PRKAA1 / AMPK Alpha 1 Antibody LS-C162140
LifeSpan Biosciences
catalog: LS-C162140
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of PRKAA1 Antibody in K562, MDA-MB435 cell line lysates (35 ug/lane). PRKAA1 (arrow) was detected using the purified antibody.

PRKAA1 Antibody IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

PRKAA1 Antibody flow cytometry of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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PRKAA1 / AMPK Alpha 1 Antibody (clone 2B7) LS-C169322
LifeSpan Biosciences
catalog: LS-C169322
mouse monoclonal (2B7)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
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PRKAA1 / AMPK Alpha 1 Antibody (Asp168) LS-C175914
LifeSpan Biosciences
catalog: LS-C175914
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of AMPK1/2 (D168) pAb in extracts from COS7 treated with Adriamycin 0.5ng/ml 24h.

IHC of AMPK1/2 (D168) pAb in paraffin-embedded human breast carcinoma tissue.
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PRKAA1 / AMPK Alpha 1 Antibody (phospho-Ser486) LS-C177871
LifeSpan Biosciences
catalog: LS-C177871
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-AMPK1 (S487) pAb in extracts from HeLa ,PC12 and Raw264.7 cells.

IHC of p-AMPK1 (S487) pAb in paraffin-embedded human colon carcinoma tissue.
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PRKAA1 / AMPK Alpha 1 Antibody (phospho-Thr174) LS-C178072
LifeSpan Biosciences
catalog: LS-C178072
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-AMPK1 (T174)pAb in extracts from HUVEC cells.

IHC of p-AMPK1 (T174) pAb in paraffin-embedded human breast carcinoma tissue.
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PRKAA1 / AMPK Alpha 1 Antibody (Asn180) LS-C178156
LifeSpan Biosciences
catalog: LS-C178156
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of AMPK1 (N180) pAb in extracts from HepG2 cells.

IHC of AMPK1 (N180) pAb in paraffin-embedded human breast carcinoma tissue.
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PRKAA1 / AMPK Alpha 1 Antibody (Arg493) LS-C178203
LifeSpan Biosciences
catalog: LS-C178203
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of AMPK1/2 (R493) pAb in paraffin-embedded human lung adenocarcinoma tissue.
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PRKAA1 / AMPK Alpha 1 Antibody (N-Terminus, clone 84.1) LS-C188362
LifeSpan Biosciences
catalog: LS-C188362
mouse monoclonal (84.1)
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
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PRKAA1 / AMPK Alpha 1 Antibody (N-Terminus, clone 34.2) LS-C188363
LifeSpan Biosciences
catalog: LS-C188363
mouse monoclonal (34.2)
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
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PRKAA1 + PRKAA2 Antibody (phospho-Thr183/172) LS-C192188
LifeSpan Biosciences
catalog: LS-C192188
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-AMPK alpha 1/2 (T183/172) pAb in extracts from COS7 cells treated with heat shock.

Immunohistochemistry (IHC) analysis of p-AMPK alpha 1/2 (T183/172) pAb in paraffin-embedded human breast cancer tissue.
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PRKAA1 / AMPK Alpha 1 Antibody (clone 167CT22.1.6, AP) LS-C206353
LifeSpan Biosciences
catalog: LS-C206353
mouse monoclonal (167CT22.1.6)
reactivity: human
conjugate: AP
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (clone 167CT22.1.6, APC) LS-C206354
LifeSpan Biosciences
catalog: LS-C206354
mouse monoclonal (167CT22.1.6)
reactivity: human
conjugate: APC
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (clone 167CT22.1.6, Biotin) LS-C206355
LifeSpan Biosciences
catalog: LS-C206355
mouse monoclonal (167CT22.1.6)
reactivity: human
conjugate: biotin
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (clone 167CT22.1.6, FITC) LS-C206357
LifeSpan Biosciences
catalog: LS-C206357
mouse monoclonal (167CT22.1.6)
reactivity: human
conjugate: FITC
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (clone 167CT22.1.6, PE) LS-C206360
LifeSpan Biosciences
catalog: LS-C206360
mouse monoclonal (167CT22.1.6)
reactivity: human
conjugate: PE
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (Ser487, AP) LS-C206387
LifeSpan Biosciences
catalog: LS-C206387
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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PRKAA1 / AMPK Alpha 1 Antibody (Ser487, APC) LS-C206388
LifeSpan Biosciences
catalog: LS-C206388
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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PRKAA1 / AMPK Alpha 1 Antibody (Ser487, Biotin) LS-C206389
LifeSpan Biosciences
catalog: LS-C206389
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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PRKAA1 / AMPK Alpha 1 Antibody (Ser487, FITC) LS-C206390
LifeSpan Biosciences
catalog: LS-C206390
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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PRKAA1 / AMPK Alpha 1 Antibody (Ser487, PE) LS-C206391
LifeSpan Biosciences
catalog: LS-C206391
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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PRKAA1 / AMPK Alpha 1 Antibody (aa479-510, AP) LS-C206392
LifeSpan Biosciences
catalog: LS-C206392
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (aa479-510, Biotin) LS-C206393
LifeSpan Biosciences
catalog: LS-C206393
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (aa479-510, APC) LS-C206394
LifeSpan Biosciences
catalog: LS-C206394
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (aa479-510, FITC) LS-C206395
LifeSpan Biosciences
catalog: LS-C206395
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (AP) LS-C206396
LifeSpan Biosciences
catalog: LS-C206396
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (aa479-510, PE) LS-C206397
LifeSpan Biosciences
catalog: LS-C206397
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (APC) LS-C206398
LifeSpan Biosciences
catalog: LS-C206398
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (Biotin) LS-C206399
LifeSpan Biosciences
catalog: LS-C206399
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (FITC) LS-C206400
LifeSpan Biosciences
catalog: LS-C206400
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (PE) LS-C206405
LifeSpan Biosciences
catalog: LS-C206405
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (clone 167CT22.1.6, Azide-free, HRP) LS-C206992
LifeSpan Biosciences
catalog: LS-C206992
mouse monoclonal (167CT22.1.6)
reactivity: human
conjugate: HRP
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (Ser487, Azide-free, HRP) LS-C207936
LifeSpan Biosciences
catalog: LS-C207936
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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PRKAA1 / AMPK Alpha 1 Antibody (aa479-510, Azide-free, HRP) LS-C207939
LifeSpan Biosciences
catalog: LS-C207939
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (Azide-free, HRP) LS-C207941
LifeSpan Biosciences
catalog: LS-C207941
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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IHC-plus™ PRKAA1 / AMPK Alpha 1 Antibody (aa110-159) LS-B10326
LifeSpan Biosciences
catalog: LS-B10326
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PRKAA1 / AMPK Alpha 1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PRKAA1 / AMPK Alpha 1 Antibody (aa350-400) LS-C285909
LifeSpan Biosciences
catalog: LS-C285909
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section
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PRKAA1 / AMPK Alpha 1 Antibody LS-C192491
LifeSpan Biosciences
catalog: LS-C192491
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of PRKAA1 pAb in extracts from Hela cells.
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PRKAA1 / AMPK Alpha 1 Antibody (clone 167CT22.1.6) LS-C319200
LifeSpan Biosciences
catalog: LS-C319200
mouse monoclonal (167CT22.1.6)
reactivity: human
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (aa479-510) LS-C326835
LifeSpan Biosciences
catalog: LS-C326835
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody LS-C326837
LifeSpan Biosciences
catalog: LS-C326837
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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PRKAA1 / AMPK Alpha 1 Antibody (Ser487) LS-C326838
LifeSpan Biosciences
catalog: LS-C326838
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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PRKAA1 / AMPK Alpha 1 Antibody LS-C331372
LifeSpan Biosciences
catalog: LS-C331372
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines, using PRKAA1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

Immunohistochemistry of paraffin-embedded human liver cancer using PRKAA1 antibody at dilution of 1:200 (x400 lens).

Immunofluorescence analysis of HeLa cell using PRKAA1 antibody.
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PRKAA1 / AMPK Alpha 1 Antibody (phospho-Thr174) LS-C336065
LifeSpan Biosciences
catalog: LS-C336065
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue.
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PRKAA1 / AMPK Alpha 1 Antibody (phospho-Ser496) LS-C354066
LifeSpan Biosciences
catalog: LS-C354066
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of AMPK alpha 1 (pS496) expression in HUVEC UV-treated (A); HeLa (B); SP2/0 (C); PC12 (D) whole cell lysates.

Immunohistochemical analysis of AMPK alpha 1 (pS496) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of AMPK alpha 1 (pS496) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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PRKAA1 / AMPK Alpha 1 Antibody (clone 2B7) LS-C359330
LifeSpan Biosciences
catalog: LS-C359330
mouse monoclonal (2B7)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
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PRKAA1 / AMPK Alpha 1 Antibody (420-500 aa) LS-C380610
LifeSpan Biosciences
catalog: LS-C380610
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-AMPKalpha1 (S496) antibody
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PRKAA1 / AMPK Alpha 1 Antibody (420-500 aa) LS-C382033
LifeSpan Biosciences
catalog: LS-C382033
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of AMPKalpha1 antibody
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PRKAA1 / AMPK Alpha 1 Antibody LS-C387767
LifeSpan Biosciences
catalog: LS-C387767
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of AMPKalpha1 antibody
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PRKAA1 / AMPK Alpha 1 Antibody LS-C387838
LifeSpan Biosciences
catalog: LS-C387838
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Immunohistochemistry of AMPKalpha1 antibody

Western blot of AMPKalpha1 antibody
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