PPP2R2A Antibody (aa398-411) LS-C38592
LifeSpan Biosciences
catalog: LS-C38592

mouse monoclonal
reactivity: human
application: western blot, immunoprecipitation

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PPP2R2A Antibody (C-Terminus) LS-C170243
LifeSpan Biosciences
catalog: LS-C170243

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

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PPP2R2A Antibody (aa43-71) LS-C204050
LifeSpan Biosciences
catalog: LS-C204050

domestic rabbit polyclonal
reactivity: human
application: western blot

PPP2R2A Antibody western blot of ZR-75-1 cell line lysates (35 ug/lane). The PPP2R2A antibody detected the PPP2R2A protein (arrow).
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PPP2R2A Antibody LS-C193096
LifeSpan Biosciences
catalog: LS-C193096

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of rat brain cell lines, using PPP2R2A antibody.
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PPP2R2A Antibody LS-C335210
LifeSpan Biosciences
catalog: LS-C335210

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines, using PPP2R2A antibody.

Immunohistochemistry of paraffin-embedded rat brain using PPP2R2A antibody at dilution of 1:100 (200x lens).

Immunofluorescence analysis of U2OS cell using PPP2R2A antibody. Blue: DAPI for nuclear staining.
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PPP2R2A Antibody (N-Terminus) LS-C345701
LifeSpan Biosciences
catalog: LS-C345701

domestic rabbit polyclonal
reactivity: human
application: western blot

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PPP2R2A Antibody LS-C482360
LifeSpan Biosciences
catalog: LS-C482360

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of PPP2R2A expression in HeLa (A); Jurkat (B); mouse brain (C) whole cell lysates.

Immunohistochemical analysis of PPP2R2A staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PPP2R2A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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